top of page
mmu_circMe3_322
Basic information of circular RNA
PanCircBase ID:
mmu_circMe3_322
mm39_Chr. Position ID:
chr7|89482904|89495521|+
Gene name:
Me3
Transcript:
ENSMUST00000032856.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,8,9
Exon/intron offsets:
0,12098,12503
Genomic length:
12617
Exon/intron sizes in nt:
1,10,98,114
Flanking exon/introns:
chr7:89455958-89482904|chr7:89495521-89497131
Splice length:
322
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTCGGAATAAATTGCCTCATCCAGTTCGAAGACTTTGCCAATGCCAATGCCTTCCGTCTACTCAACAAATACCGCAACAAGTACTGTATGTTCAATGATGACATCCAAGGCACAGCCTCTGTCGCTGTGGCAGGGATCCTGGCTGCTCTGAGAATCACCAAGAACAGACTGTCCAATCATGTGTTTGTTTTCCAGGGTGCGGGTGAGGCAGCCATGGGCATTGCCCACCTCCTTGTCATGGCCCTGGAAAAGGAAGGCATACCCAAGACAGAGGCCATAAAGAAGATCTGGATGGTGGACTCCAAGGGTCTGATTGTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCATGGCCCTGGAAAAGG
60.25
AGTAGACGGAAGGCATTGGC
60.11
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTTGTCATGGCCCTGGAAA
60.25
AGTAGACGGAAGGCATTGGC
60.11
154
PCR template sequence:
TGCCCACCTCCTTGTCATGGCCCTGGAAAAGGAAGGCATACCCAAGACAGAGGCCATAAAGAAGATCTGGATGGTGGACTCCAAGGGTCTGATTGTCAAGGTTCGGAATAAATTGCCTCATCCAGTTCGAAGACTTTGCCAATGCCAATGCCTTCCGTCTACTCAACAAATACCGCAACAAGTACTGTATGTTCAATGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-101b-3p(1), mmu-miR-495-3p(1), mmu-miR-182-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTGATTGTCAAGGTTCGGAATAAAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCME3_322_ORF_1
1.095
0.15839313
0.457300362
333
ORF sequence:
ATGTTCAATGATGACATCCAAGGCACAGCCTCTGTCGCTGTGGCAGGGATCCTGGCTGCTCTGAGAATCACCAAGAACAGACTGTCCAATCATGTGTTTGTTTTCCAGGGTGCGGGTGAGGCAGCCATGGGCATTGCCCACCTCCTTGTCATGGCCCTGGAAAAGGAAGGCATACCCAAGACAGAGGCCATAAAGAAGATCTGGATGGTGGACTCCAAGGGTCTGATTGTCAAGGTTCGGAATAAATTGCCTCATCCAGTTCGAAGACTTTGCCAATGCCAATGCCTTCCGTCTACTCAACAAATACCGCAACAAGTACTGTATGTTCAATGA
Peptide sequence:
MFNDDIQGTASVAVAGILAALRITKNRLSNHVFVFQGAGEAAMGIAHLLVMALEKEGIPKTEAIKKIWMVDSKGLIVKVRNKLPHPVRRLCQCQCLPSTQQIPQQVLYVQ
IRESfinder result
IRESfinder score
0.294510873
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page