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mmu_circMe2_229
Basic information of circular RNA
PanCircBase ID:
mmu_circMe2_229
mm39_Chr. Position ID:
chr18|73921424|73924268|-
Gene name:
Me2
Transcript:
ENSMUST00000025439.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
11,10
Exon/intron offsets:
0,2730
Genomic length:
2844
Exon/intron sizes in nt:
1,15,114
Flanking exon/introns:
chr18:73918908-73921424|chr18:73924268-73924679
Splice length:
229
Other database IDs:
circAtlas: mmu-Me2_0018
Human Ortholog IDs:
Mature spliced Sequence:
GCTGCACTTGGAATTGCCAATCTCATAGTTCTATCAATGGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGACAAGAGTGGCTTGCTGGTTAAGGGGCGGACTGCTAGCATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATACCTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAACCATATGCCCATGCG
60.54
TCCAGATCTTTCTCTGCGCC
59.82
172
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAACCATATGCCCATGCG
60.54
CCAGATCTTTCTCTGCGCCT
59.82
171
PCR template sequence:
ATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATACCTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTGGCTGCACTTGGAATTGCCAATCTCATAGTTCTATCAATGGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGACAAGAGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TCAGTTATAATTGGCTGCACTTGGAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCME2_229_ORF_1
0.8283
0.041506545
0.184351919
285
ORF sequence:
ATGGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGACAAGAGTGGCTTGCTGGTTAAGGGGCGGACTGCTAGCATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATACCTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTGGCTGCACTTGGAATTGCCAATCTCATAGTTCTATCAATGGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGA
Peptide sequence:
MVESGLSEEEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGCTWNCQSHSSINGGKWSLRRGGAEKDLDV
IRESfinder result
IRESfinder score
0.586662811
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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