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mmu_circMe2_1063
Basic information of circular RNA
PanCircBase ID:
mmu_circMe2_1063
mm39_Chr. Position ID:
chr18|73921424|73934933|-
Gene name:
Me2
Transcript:
ENSMUST00000025439.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,2730,3255,3447,6092,6427,7960,9475,13375
Genomic length:
13509
Exon/intron sizes in nt:
11,51,14,98,11,01,04,10,00,00,00,000
Flanking exon/introns:
chr18:73918908-73921424|chr18:73934933-73938122
Splice length:
1063
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGAATGGCGTTTACGTTACAAGAGCGACAAATGCTTGGTCTTCAAGGACTTTTACCTCCTAAAATAGAGACACAGGATATTCAAGCCTTACGATTCCATAGAAACTTGAAAAAAATGACTAGCCCTTTGGAAAAATACATTTATATAATGGGCATCCAAGAAAGAAATGAGAAACTGTTCTATAGAATCCTGCAGGATGATATTGAAAGTCTGATGCCGATTGTGTACACGCCAACAGTTGGTCTTGCCTGCTGTCAATATGGGCACATCTTTAGGAGACCTAAGGGATTGTTTATTTCAATCTCAGACAGAGGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTGGTGACTGATGGAGAGAGGATCCTGGGCCTTGGCGATCTGGGTGTCTATGGAATGGGGATTCCAGTAGGGAAGCTGTGCCTGTATACAGCCTGTGCAGGGATACAGCCCGAGAAGTGCCTGCCCGTGTGCATCGACGTGGGCACTGATAACATGGCACTATTAAAAGATCCGTTTTATATGGGCTTGTATCAGAAGCGAGATCGTTCCCAGCTCTATGATGATCTGATGGACGAGTTTATGAAGGCGATTACTGACAGATACGGCCGGAACACACTCATTCAGTTTGAAGACTTTGGAAATCATAATGCATTCAGGTTCTTAAGAAAATATCAACAAAAATACTGTACCTTCAATGATGACATTCAAGGGACAGCTGCAGTTGCTCTGTCTGGTCTTCTTGCGGCCCAAAGAGTTATTAATAAACCAGTCTCAGAACACAAAATCTTATTTCTTGGAGCAGGAGAAGCTGCACTTGGAATTGCCAATCTCATAGTTCTATCAATGGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGACAAGAGTGGCTTGCTGGTTAAGGGGCGGACTGCTAGCATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATACCTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAACCATATGCCCATGCG
60.54
AGGCTTGAATATCCTGTGTCTCT
58.96
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAACCATATGCCCATGCG
60.54
AGGAGGTAAAAGTCCTTGAAGACC
59.96
147
PCR template sequence:
ATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATACCTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTGGGAATGGCGTTTACGTTACAAGAGCGACAAATGCTTGGTCTTCAAGGACTTTTACCTCCTAAAATAGAGACACAGGATATTCAAGCCTTACGATTCCATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-214-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-383-5p(1), mmu-miR-207(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGTTATAATTGGGAATGGCGTTTA
siRNA sets
AAACGCCATTCCCAATTAT, TAAACGCCATTCCCAATTA, CATTCCCAATTATAACTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCME2_1063_ORF_1
0.9108
0.063081103
0.99146997
1122
ORF sequence:
ATGGCGTTTACGTTACAAGAGCGACAAATGCTTGGTCTTCAAGGACTTTTACCTCCTAAAATAGAGACACAGGATATTCAAGCCTTACGATTCCATAGAAACTTGAAAAAAATGACTAGCCCTTTGGAAAAATACATTTATATAATGGGCATCCAAGAAAGAAATGAGAAACTGTTCTATAGAATCCTGCAGGATGATATTGAAAGTCTGATGCCGATTGTGTACACGCCAACAGTTGGTCTTGCCTGCTGTCAATATGGGCACATCTTTAGGAGACCTAAGGGATTGTTTATTTCAATCTCAGACAGAGGTCATGTTAGATCAATTGTGGATAACTGGCCAGAAAATCATGTTAAGGCTGTTGTGGTGACTGATGGAGAGAGGATCCTGGGCCTTGGCGATCTGGGTGTCTATGGAATGGGGATTCCAGTAGGGAAGCTGTGCCTGTATACAGCCTGTGCAGGGATACAGCCCGAGAAGTGCCTGCCCGTGTGCATCGACGTGGGCACTGATAACATGGCACTATTAAAAGATCCGTTTTATATGGGCTTGTATCAGAAGCGAGATCGTTCCCAGCTCTATGATGATCTGATGGACGAGTTTATGAAGGCGATTACTGACAGATACGGCCGGAACACACTCATTCAGTTTGAAGACTTTGGAAATCATAATGCATTCAGGTTCTTAAGAAAATATCAACAAAAATACTGTACCTTCAATGATGACATTCAAGGGACAGCTGCAGTTGCTCTGTCTGGTCTTCTTGCGGCCCAAAGAGTTATTAATAAACCAGTCTCAGAACACAAAATCTTATTTCTTGGAGCAGGAGAAGCTGCACTTGGAATTGCCAATCTCATAGTTCTATCAATGGTGGAAAGTGGTCTCTCAGAAGAGGAGGCGCAGAGAAAGATCTGGATGTTTGACAAGAGTGGCTTGCTGGTTAAGGGGCGGACTGCTAGCATAGATAGCAACCAGGAACCATATGCCCATGCGGCCCCAGAGAGCATACCTGCGACTTTTGAAGATGCAGTAAATAAACTTAAGCCTTCAGTTATAATTGGGAATGGCGTTTACGTTACAAGAGCGACAAATGCTTGGTCTTCAAGGACTTTTACCTCCTAA
Peptide sequence:
MAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDIQGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGNGVYVTRATNAWSSRTFTS
IRESfinder result
IRESfinder score
0.619783074
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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