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mmu_circMcm6_432
Basic information of circular RNA
PanCircBase ID:
mmu_circMcm6_432
mm39_Chr. Position ID:
chr1|128261253|128263602|-
Gene name:
Mcm6
Transcript:
ENSMUST00000027601.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
16,15,14
Exon/intron offsets:
0,1246,2213
Genomic length:
2349
Exon/intron sizes in nt:
14,01,56,136
Flanking exon/introns:
chr1:128259788-128261253|chr1:128263602-128265852
Splice length:
432
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTCAGCCTAAACATGTGAAGGAAGCTTTTCGATTACTGAATAAATCAATCATCCGTGTAGAGACGCCTGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGACGGATGAGGGACAAGGAGGCGTCAATGGTCATGCCGACAGCCCTGCCCCTGTGAATAGGTTCAACGGCTCCAGTGAAGATGCCAGCCAGGAGACTGTTTCCAAGCCCTCCCTGAGGCTGGGCTTTGCTGAATACTGCCGAATCTCGAACCTCATCGTGCTCCACCTCAGGAAAATGGAAGAAGAGGAGGACGAGTCGGCACTAAAGAGAAGCGAGCTTGTCAACTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAGCTCATAAATAAAAAGACGATCATTGAGAAGGTTGTTCATCGTCTCACACACTAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGGTACCTGAAGGAAATCGA
57.11
CAGGCGTCTCTACACGGATG
60.25
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGGTACCTGAAGGAAATCGA
57.11
CATCAGGCGTCTCTACACGG
60.25
173
PCR template sequence:
CTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAGCTCATAAATAAAAAGACGATCATTGAGAAGGTTGTTCATCGTCTCACACACTACGTTCAGCCTAAACATGTGAAGGAAGCTTTTCGATTACTGAATAAATCAATCATCCGTGTAGAGACGCCTGATGTCAATCTAGATCAAGAGGAAGAGATCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-132-3p(1), mmu-miR-212-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTCACACACTACGTTCAGCCTAAAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMCM6_432_ORF_1
1.2621
0.437313821
0.987513985
762
ORF sequence:
ATGGAGACGGATGAGGGACAAGGAGGCGTCAATGGTCATGCCGACAGCCCTGCCCCTGTGAATAGGTTCAACGGCTCCAGTGAAGATGCCAGCCAGGAGACTGTTTCCAAGCCCTCCCTGAGGCTGGGCTTTGCTGAATACTGCCGAATCTCGAACCTCATCGTGCTCCACCTCAGGAAAATGGAAGAAGAGGAGGACGAGTCGGCACTAAAGAGAAGCGAGCTTGTCAACTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAGCTCATAAATAAAAAGACGATCATTGAGAAGGTTGTTCATCGTCTCACACACTACGTTCAGCCTAAACATGTGAAGGAAGCTTTTCGATTACTGAATAAATCAATCATCCGTGTAGAGACGCCTGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGACGGATGAGGGACAAGGAGGCGTCAATGGTCATGCCGACAGCCCTGCCCCTGTGAATAGGTTCAACGGCTCCAGTGAAGATGCCAGCCAGGAGACTGTTTCCAAGCCCTCCCTGAGGCTGGGCTTTGCTGAATACTGCCGAATCTCGAACCTCATCGTGCTCCACCTCAGGAAAATGGAAGAAGAGGAGGACGAGTCGGCACTAAAGAGAAGCGAGCTTGTCAACTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAGCTCATAAATAAAAAGACGATCATTGAGAAGGTTGTTCATCGTCTCACACACTAC
Peptide sequence:
METDEGQGGVNGHADSPAPVNRFNGSSEDASQETVSKPSLRLGFAEYCRISNLIVLHLRKMEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKTIIEKVVHRLTHYVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMETDEGQGGVNGHADSPAPVNRFNGSSEDASQETVSKPSLRLGFAEYCRISNLIVLHLRKMEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKTIIEKVVHRLTHY
IRESfinder result
IRESfinder score
0.346750307
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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