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mmu_circMbtps2_306
Basic information of circular RNA
PanCircBase ID:
mmu_circMbtps2_306
mm39_Chr. Position ID:
chrX|156359079|156374551|-
Gene name:
Mbtps2
Transcript:
ENSMUST00000058098.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,3
Exon/intron offsets:
0,15270
Genomic length:
15472
Exon/intron sizes in nt:
1,04,202
Flanking exon/introns:
chrX:156355982-156359079|chrX:156374551-156376720
Splice length:
306
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTCAATTTTGGAATGGTGTTTGGCGTGATTGCCATGTTCAGCTCTTTTTTTCTCCTTGGAAAAACACTGATGCAGACTTTGGCACAAATGATGGCTGACTCTCCCTCTCCTTATTCTTCCTCTTCCTCTTCCTCCTCTTCCTCTTCCTCCTCTTCCTCTTCCTCCTCCTCTCTTCACAATGAACAGGTGTTGCAAGTTGTGGTTCCCGGTATAAATTTACCTGTCAATCAGCTGACTTACTTCTTCGCTGCAGTCCTCATTAGTGGTGTTGTACATGAAATTGGACACGGTATAGCAGCTATTAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTTCTTCGCTGCAGTCCTC
60.04
GTCAGCCATCATTTGTGCCA
59.11
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTTCTTCGCTGCAGTCCTCA
60.04
GTCAGCCATCATTTGTGCCA
59.11
166
PCR template sequence:
CCGGTATAAATTTACCTGTCAATCAGCTGACTTACTTCTTCGCTGCAGTCCTCATTAGTGGTGTTGTACATGAAATTGGACACGGTATAGCAGCTATTAGGTTCAATTTTGGAATGGTGTTTGGCGTGATTGCCATGTTCAGCTCTTTTTTTCTCCTTGGAAAAACACTGATGCAGACTTTGGCACAAATGATGGCTGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-29a-3p(1), mmu-miR-29c-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TAGCAGCTATTAGGTTCAATTTTGGA
siRNA sets
AATTGAACCTAATAGCTGC,AAAATTGAACCTAATAGCT,AAATTGAACCTAATAGCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMBTPS2_306_ORF_1
0.8187
-0.017864935
0.622498402
597
ORF sequence:
ATGGTGTTTGGCGTGATTGCCATGTTCAGCTCTTTTTTTCTCCTTGGAAAAACACTGATGCAGACTTTGGCACAAATGATGGCTGACTCTCCCTCTCCTTATTCTTCCTCTTCCTCTTCCTCCTCTTCCTCTTCCTCCTCTTCCTCTTCCTCCTCCTCTCTTCACAATGAACAGGTGTTGCAAGTTGTGGTTCCCGGTATAAATTTACCTGTCAATCAGCTGACTTACTTCTTCGCTGCAGTCCTCATTAGTGGTGTTGTACATGAAATTGGACACGGTATAGCAGCTATTAGGTTCAATTTTGGAATGGTGTTTGGCGTGATTGCCATGTTCAGCTCTTTTTTTCTCCTTGGAAAAACACTGATGCAGACTTTGGCACAAATGATGGCTGACTCTCCCTCTCCTTATTCTTCCTCTTCCTCTTCCTCCTCTTCCTCTTCCTCCTCTTCCTCTTCCTCCTCCTCTCTTCACAATGAACAGGTGTTGCAAGTTGTGGTTCCCGGTATAAATTTACCTGTCAATCAGCTGACTTACTTCTTCGCTGCAGTCCTCATTAGTGGTGTTGTACATGAAATTGGACACGGTATAGCAGCTATT
Peptide sequence:
MVFGVIAMFSSFFLLGKTLMQTLAQMMADSPSPYSSSSSSSSSSSSSSSSSSSLHNEQVLQVVVPGINLPVNQLTYFFAAVLISGVVHEIGHGIAAIRFNFGMVFGVIAMFSSFFLLGKTLMQTLAQMMADSPSPYSSSSSSSSSSSSSSSSSSSLHNEQVLQVVVPGINLPVNQLTYFFAAVLISGVVHEIGHGIAAI
IRESfinder result
IRESfinder score
0.826531321
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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