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mmu_circMbtd1_1873
Basic information of circular RNA
PanCircBase ID:
mmu_circMbtd1_1873
mm39_Chr. Position ID:
chr11|93782164|93824784|+
Gene name:
Mbtd1
Transcript:
ENSMUST00000063718.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
15
Exon/intron indexes:
3,4,5,6,7,8,9,10,11,12,13,14,15,16,17
Exon/intron offsets:
0,13820,17465,18944,21037,30028,31807,32452,33086,34328,34883,38275,40474,40881,42542
Genomic length:
42620
Exon/intron sizes in nt:
50,200,134,124,83,118,135,89,235,56,105,148,83,235,78
Flanking exon/introns:
chr11:93777899-93782164|chr11:93824784-93834487
Splice length:
1873
Other database IDs:
circAtlas: mmu-Mbtd1_0028
Human Ortholog IDs:
Mature spliced Sequence:
GGTTTCTGTAAGCCTCCACTCAGAGATGTGATGATCACTGCAATTAAAAAACAGAACATTCTTCACAATCCAAAAGAAAAAGCAGACTGGTTCGGGATGTTTGACGGCTATGATAGCTGCAGTGAGGACACAAGTAGCAGCTCCAGCTCTGAGGAGAGTGAAGAAGAAGTTGCTCCTTTACCTTCCAATCTCCCAATCATCAAGAATAATGGACAAGTCTACACATACCCAGATGGTAAATCTGGCATGGCTACCTGTGAGATGTGTGGGATGGTCGGTGTGCGAGATGCTTTTTACTCTAAAACGAAACGTTTCTGCAGTGTTTCCTGTTCAAGAAGTTACTCGTCAAACTCTAAGAAGGCAAGCATTCTGGCCAGACTTCAGGGTAAGCCTCCAACAAAGAAAGCAAAAGTCCTTCAAAAACAACCTTTAGTTGCTAAATTAGCTGCCTATGCCCAGTATCAAGCTACCTTGCAAAATCAAGCAAAGACAAAAGCAGGCAATTCTGCAATCTCTGTGGAAGGCTTCAGCTGGGGTAACTACATCAACAGCAACAGCTTCATAGCAGCTCCAGTGGCCTGTTTTAAGCATGCACCTATGGGGACCTGCTGGGGTGATATCTCGGAAAATGTAAGGATAGAAGTTCCCAATACAGACTGCAGTCTACCTACCAAAGTCTTCTGGATTGCTGGAATTATAAAATTAGCAGGTTATAATGCCCTTTTGAGATATGAAGGATTTGAAAATGATTCTTCTCTGGACTTCTGGTGCAATATATGTGGGTCTGATATTCATCCAGTTGGTTGGTGTGCAGCTAGTGGAAAACCTCTCGTTCCTCCTAGAACTGTTCAACATAAATATACAAACTGGAAAGCTTTTCTAGTAAAAAGACTTACTGGTGCCAAAACACTTCCTCCTGATTTCTCACAAAAGGTTTCTGAGAGTATGCAATATCCTTTCAAACCTTGCATGAGAGTAGAAGTAGTTGACAAGAGGCATTTATGTCGAACAAGAGTAGCAGTGGTGGAAAGTGTAATTGGAGGACGACTACGGCTGGTGTATGAAGAGAGTGAAGATAGAACAGACGACTTCTGGTGCCACATGCACAGCCCCTTAATCCACCATATTGGATGGTCAAGAAGCATAGGCCATCGATTCAAGAGATCAGATATTACGAAGAAACAGGACGGACATTTCGATACACCTCCACACTTATTTGCTAAGGTAAAAGAAGTAGACCAGAGTGGAGAATGGTTCAAAGAAGGAATGAAATTGGAAGCTATAGACCCATTAAATCTTTCTACAATATGTGTTGCCACCATTAGAAAGGTGCTGGCTGATGGATTCCTGATGATTGGGATTGATGGCTCAGAAGCAGCAGATGGATCTGACTGGTTCTGTTATCATGCAACCTCTCCTTCCATTTTCCCTGTGGGTTTCTGTGAAATTAACATGATAGAACTGACTCCACCCAGAGGTTACACAAAACTTCCTTTTAAATGGTTTGACTACCTCAGGGAAACCGGCTCCATTGCAGCACCAGTAAAACTATTTAATAAGGATGTTCCAAACCACGGATTCCGTGTAGGAATGAAATTAGAAGCTGTAGATCTCATGGAGCCACGGTTAATATGTGTAGCCACAGTTACTCGAATTATTCACCGTCTCTTGAGGATACATTTTGATGGTTGGGAAGAAGAGTATGACCAGTGGGTAGACTGTGAGTCCCCTGACCTCTATCCTGTAGGGTGGTGTCAGTTAACTGGATATCAACTACAGCCTCCAGCATCACAGTCATCAAGAGAAAGCCAATCAGCTTCTTCAAAACAGAAGAAAAAGGCTAAGTCTCAGCAATACAAAGGACATAAGAAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCAATCAGCTTCTTCAAAACA
59.36
CATCCCGAACCAGTCTGCTT
60.04
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCATCAAGAGAAAGCCAATCAGC
59.31
CATCCCGAACCAGTCTGCTT
60.04
178
PCR template sequence:
CTACAGCCTCCAGCATCACAGTCATCAAGAGAAAGCCAATCAGCTTCTTCAAAACAGAAGAAAAAGGCTAAGTCTCAGCAATACAAAGGACATAAGAAAAGGTTTCTGTAAGCCTCCACTCAGAGATGTGATGATCACTGCAATTAAAAAACAGAACATTCTTCACAATCCAAAAGAAAAAGCAGACTGGTTCGGGATGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
23
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-137-3p(1), mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-miR-200a-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-223-3p(1), mmu-miR-205-5p(1), mmu-let-7c-5p(1), mmu-miR-196a-5p(1), mmu-miR-214-3p(1), mmu-let-7d-5p(1), mmu-miR-136-5p(1), mmu-miR-434-3p(1), mmu-miR-127-3p(1), mmu-miR-351-5p(1), mmu-miR-363-3p(1), mmu-miR-125a-5p(1), mmu-miR-96-5p(1), mmu-miR-98-5p(1), mmu-miR-669d-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGACATAAGAAAAGGTTTCTGTAAGC
siRNA sets
GAAACCTTTTCTTATGTCC, TTACAGAAACCTTTTCTTA, AGAAACCTTTTCTTATGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMBTD1_1873_ORF_1
0.7364
0.016578939
0.999924071
1854
ORF sequence:
ATGATCACTGCAATTAAAAAACAGAACATTCTTCACAATCCAAAAGAAAAAGCAGACTGGTTCGGGATGTTTGACGGCTATGATAGCTGCAGTGAGGACACAAGTAGCAGCTCCAGCTCTGAGGAGAGTGAAGAAGAAGTTGCTCCTTTACCTTCCAATCTCCCAATCATCAAGAATAATGGACAAGTCTACACATACCCAGATGGTAAATCTGGCATGGCTACCTGTGAGATGTGTGGGATGGTCGGTGTGCGAGATGCTTTTTACTCTAAAACGAAACGTTTCTGCAGTGTTTCCTGTTCAAGAAGTTACTCGTCAAACTCTAAGAAGGCAAGCATTCTGGCCAGACTTCAGGGTAAGCCTCCAACAAAGAAAGCAAAAGTCCTTCAAAAACAACCTTTAGTTGCTAAATTAGCTGCCTATGCCCAGTATCAAGCTACCTTGCAAAATCAAGCAAAGACAAAAGCAGGCAATTCTGCAATCTCTGTGGAAGGCTTCAGCTGGGGTAACTACATCAACAGCAACAGCTTCATAGCAGCTCCAGTGGCCTGTTTTAAGCATGCACCTATGGGGACCTGCTGGGGTGATATCTCGGAAAATGTAAGGATAGAAGTTCCCAATACAGACTGCAGTCTACCTACCAAAGTCTTCTGGATTGCTGGAATTATAAAATTAGCAGGTTATAATGCCCTTTTGAGATATGAAGGATTTGAAAATGATTCTTCTCTGGACTTCTGGTGCAATATATGTGGGTCTGATATTCATCCAGTTGGTTGGTGTGCAGCTAGTGGAAAACCTCTCGTTCCTCCTAGAACTGTTCAACATAAATATACAAACTGGAAAGCTTTTCTAGTAAAAAGACTTACTGGTGCCAAAACACTTCCTCCTGATTTCTCACAAAAGGTTTCTGAGAGTATGCAATATCCTTTCAAACCTTGCATGAGAGTAGAAGTAGTTGACAAGAGGCATTTATGTCGAACAAGAGTAGCAGTGGTGGAAAGTGTAATTGGAGGACGACTACGGCTGGTGTATGAAGAGAGTGAAGATAGAACAGACGACTTCTGGTGCCACATGCACAGCCCCTTAATCCACCATATTGGATGGTCAAGAAGCATAGGCCATCGATTCAAGAGATCAGATATTACGAAGAAACAGGACGGACATTTCGATACACCTCCACACTTATTTGCTAAGGTAAAAGAAGTAGACCAGAGTGGAGAATGGTTCAAAGAAGGAATGAAATTGGAAGCTATAGACCCATTAAATCTTTCTACAATATGTGTTGCCACCATTAGAAAGGTGCTGGCTGATGGATTCCTGATGATTGGGATTGATGGCTCAGAAGCAGCAGATGGATCTGACTGGTTCTGTTATCATGCAACCTCTCCTTCCATTTTCCCTGTGGGTTTCTGTGAAATTAACATGATAGAACTGACTCCACCCAGAGGTTACACAAAACTTCCTTTTAAATGGTTTGACTACCTCAGGGAAACCGGCTCCATTGCAGCACCAGTAAAACTATTTAATAAGGATGTTCCAAACCACGGATTCCGTGTAGGAATGAAATTAGAAGCTGTAGATCTCATGGAGCCACGGTTAATATGTGTAGCCACAGTTACTCGAATTATTCACCGTCTCTTGAGGATACATTTTGATGGTTGGGAAGAAGAGTATGACCAGTGGGTAGACTGTGAGTCCCCTGACCTCTATCCTGTAGGGTGGTGTCAGTTAACTGGATATCAACTACAGCCTCCAGCATCACAGTCATCAAGAGAAAGCCAATCAGCTTCTTCAAAACAGAAGAAAAAGGCTAAGTCTCAGCAATACAAAGGACATAAGAAAAGGTTTCTGTAA
Peptide sequence:
MITAIKKQNILHNPKEKADWFGMFDGYDSCSEDTSSSSSSEESEEEVAPLPSNLPIIKNNGQVYTYPDGKSGMATCEMCGMVGVRDAFYSKTKRFCSVSCSRSYSSNSKKASILARLQGKPPTKKAKVLQKQPLVAKLAAYAQYQATLQNQAKTKAGNSAISVEGFSWGNYINSNSFIAAPVACFKHAPMGTCWGDISENVRIEVPNTDCSLPTKVFWIAGIIKLAGYNALLRYEGFENDSSLDFWCNICGSDIHPVGWCAASGKPLVPPRTVQHKYTNWKAFLVKRLTGAKTLPPDFSQKVSESMQYPFKPCMRVEVVDKRHLCRTRVAVVESVIGGRLRLVYEESEDRTDDFWCHMHSPLIHHIGWSRSIGHRFKRSDITKKQDGHFDTPPHLFAKVKEVDQSGEWFKEGMKLEAIDPLNLSTICVATIRKVLADGFLMIGIDGSEAADGSDWFCYHATSPSIFPVGFCEINMIELTPPRGYTKLPFKWFDYLRETGSIAAPVKLFNKDVPNHGFRVGMKLEAVDLMEPRLICVATVTRIIHRLLRIHFDGWEEEYDQWVDCESPDLYPVGWCQLTGYQLQPPASQSSRESQSASSKQKKKAKSQQYKGHKKRFL
IRESfinder result
IRESfinder score
0.629550987
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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