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mmu_circMap4k5_480
Basic information of circular RNA
PanCircBase ID:
mmu_circMap4k5_480
mm39_Chr. Position ID:
chr12|69891129|69903119|-
Gene name:
Map4k5
Transcript:
ENSMUST00000110567.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
10,9,8,7,6,5,4
Exon/intron offsets:
0,1325,5191,6470,6598,8343,11925
Genomic length:
11990
Exon/intron sizes in nt:
63,132,73,43,48,56,65
Flanking exon/introns:
chr12:69889743-69891129|chr12:69903119-69903546
Splice length:
480
Other database IDs:
circAtlas: hsa-MAP4K5_0095
Human Ortholog IDs:
Mature spliced Sequence:
TAGGGAGAAGCTGTGGATTTGCATGGAGTACTGCGGTGGCGGATCACTTCAAGATATCTATCACGTGACTGGGCCATTATCTGAAATGCAAATAGCCTACGTCTGCAGAGAGACCTTACAGGGTCTTGCCTACTTGCATACTAAAGGCAAAATGCATAGAGATATCAAAGGTGCAAATATCTTATTAACAGACCATGGTGATGTAAAATTAGCTGATTTTGGTGTGGCTGCAAAAATTACAGCTACCATTGCAAAGAGGAAGTCCTTCATCGGCACCCCTTACTGGATGGCTCCAGAAGTTGCAGCAGTGGAAAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCCGTCGGAATAACAGCAATCGAGCTGGGAGAACTGCAGCCGCCTATGTTTGACCTGCATCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTAATTTCCAGCCTCCAAAGCTAAAGGACAAAACTAAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCGCCTATGTTTGACCTG
60.39
TCTTGAAGTGATCCGCCACC
60.04
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCCGCCTATGTTTGACCT
60.39
TCTTGAAGTGATCCGCCACC
60.04
149
PCR template sequence:
AACTGCAGCCGCCTATGTTTGACCTGCATCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTAATTTCCAGCCTCCAAAGCTAAAGGACAAAACTAAATGTAGGGAGAAGCTGTGGATTTGCATGGAGTACTGCGGTGGCGGATCACTTCAAGATATCTATCACGTGACTGGGCCATTATCTGAAATGCAAATAGCCTAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-342-5p(1), mmu-miR-129-5p(1), mmu-miR-300-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACAAAACTAAATGTAGGGAGAAGCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAP4K5_480_ORF_1
0.9702
0.14610253
0.979974177
936
ORF sequence:
ATGGAGTACTGCGGTGGCGGATCACTTCAAGATATCTATCACGTGACTGGGCCATTATCTGAAATGCAAATAGCCTACGTCTGCAGAGAGACCTTACAGGGTCTTGCCTACTTGCATACTAAAGGCAAAATGCATAGAGATATCAAAGGTGCAAATATCTTATTAACAGACCATGGTGATGTAAAATTAGCTGATTTTGGTGTGGCTGCAAAAATTACAGCTACCATTGCAAAGAGGAAGTCCTTCATCGGCACCCCTTACTGGATGGCTCCAGAAGTTGCAGCAGTGGAAAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCCGTCGGAATAACAGCAATCGAGCTGGGAGAACTGCAGCCGCCTATGTTTGACCTGCATCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTAATTTCCAGCCTCCAAAGCTAAAGGACAAAACTAAATGTAGGGAGAAGCTGTGGATTTGCATGGAGTACTGCGGTGGCGGATCACTTCAAGATATCTATCACGTGACTGGGCCATTATCTGAAATGCAAATAGCCTACGTCTGCAGAGAGACCTTACAGGGTCTTGCCTACTTGCATACTAAAGGCAAAATGCATAGAGATATCAAAGGTGCAAATATCTTATTAACAGACCATGGTGATGTAAAATTAGCTGATTTTGGTGTGGCTGCAAAAATTACAGCTACCATTGCAAAGAGGAAGTCCTTCATCGGCACCCCTTACTGGATGGCTCCAGAAGTTGCAGCAGTGGAAAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCCGTCGGAATAACAGCAATCGAGCTGGGAGAACTGCAGCCGCCTATGTTTGACCTGCATCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTAATTTCCAGCCTCCAAAGCTAAAGGACAAAACTAAA
Peptide sequence:
MEYCGGGSLQDIYHVTGPLSEMQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKCREKLWICMEYCGGGSLQDIYHVTGPLSEMQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTK
IRESfinder result
IRESfinder score
0.515408039
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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