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mmu_circMap3k7_849
Basic information of circular RNA
PanCircBase ID:
mmu_circMap3k7_849
mm39_Chr. Position ID:
chr4|31977323|31991843|+
Gene name:
Map3k7
Transcript:
ENSMUST00000037607.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
3,4,5,6,7,8,9,10
Exon/intron offsets:
0,1107,2412,4134,8362,11265,12610,14389
Genomic length:
14520
Exon/intron sizes in nt:
66,46,13,91,25,12,91,30,00,000
Flanking exon/introns:
chr4:31974933-31977323|chr4:31991843-31992386
Splice length:
849
Other database IDs:
CIRCpedia: MMU_CIRCpedia_219928, circAtlas: mmu-Map3k7_0019
Human Ortholog IDs:
Mature spliced Sequence:
CTCCGGCAGTTGTCACGTGTGAACCATCCTAACATTGTCAAGTTGTATGGAGCCTGCCTGAATCCAGTATGTCTTGTGATGGAATATGCAGAGGGGGGCTCATTGTATAATGTGCTGCATGGTGCTGAACCATTGCCTTACTACACTGCTGCTCATGCCATGAGCTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTACCTGCACAGCATGCAGCCCAAAGCGCTGATTCACAGGGACCTCAAGCCTCCAAACTTGCTGCTGGTTGCAGGAGGGACAGTTCTAAAAATCTGCGATTTTGGTACAGCTTGTGACATCCAAACACACATGACCAATAATAAAGGGAGTGCTGCTTGGATGGCGCCTGAAGTATTTGAAGGTAGCAATTACAGTGAAAAGTGTGATGTCTTCAGCTGGGGTATTATCCTCTGGGAAGTGATAACACGCCGGAAACCCTTCGATGAGATCGGTGGCCCAGCTTTCAGAATCATGTGGGCTGTTCATAATGGCACTCGACCACCACTGATCAAAAATTTACCTAAGCCCATTGAGAGCTTGATGACACGCTGTTGGTCTAAGGACCCATCTCAGCGCCCTTCAATGGAGGAAATTGTGAAAATAATGACTCACTTGATGCGGTACTTCCCAGGAGCGGATGAGCCGTTACAGTATCCTTGTCAGTACTCTGATGAAGGGCAGAGCAACTCAGCCACCAGCACAGGCTCATTCATGGACATTGCTTCTACAAATACCAGTAATAAAAGTGACACAAATATGGAACAGGTTCCTGCCACAAACGACACTATTAAACGCTTGGAGTCAAAACTTTTGAAAAACCAGGCAAAGCAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTTCCTGCCACAAACGACAC
59.97
ACATACTGGATTCAGGCAGGC
60.13
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTTCCTGCCACAAACGACAC
59.97
CATACTGGATTCAGGCAGGCT
59.86
140
PCR template sequence:
CAGTAATAAAAGTGACACAAATATGGAACAGGTTCCTGCCACAAACGACACTATTAAACGCTTGGAGTCAAAACTTTTGAAAAACCAGGCAAAGCAACAGCTCCGGCAGTTGTCACGTGTGAACCATCCTAACATTGTCAAGTTGTATGGAGCCTGCCTGAATCCAGTATGTCTTGTGATGGAATATGCAGAGGGGGGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-34c-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-30a-5p(1), mmu-miR-320-3p(1), mmu-miR-30d-5p(1), mmu-miR-26b-5p(1), mmu-miR-182-5p(1), mmu-miR-34b-5p(1), mmu-miR-27a-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-369-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCAAAGCAACAGCTCCGGCAGTTGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAP3K7_849_ORF_1
0.8706
0.157719745
0.999828627
1620
ORF sequence:
ATGGAATATGCAGAGGGGGGCTCATTGTATAATGTGCTGCATGGTGCTGAACCATTGCCTTACTACACTGCTGCTCATGCCATGAGCTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTACCTGCACAGCATGCAGCCCAAAGCGCTGATTCACAGGGACCTCAAGCCTCCAAACTTGCTGCTGGTTGCAGGAGGGACAGTTCTAAAAATCTGCGATTTTGGTACAGCTTGTGACATCCAAACACACATGACCAATAATAAAGGGAGTGCTGCTTGGATGGCGCCTGAAGTATTTGAAGGTAGCAATTACAGTGAAAAGTGTGATGTCTTCAGCTGGGGTATTATCCTCTGGGAAGTGATAACACGCCGGAAACCCTTCGATGAGATCGGTGGCCCAGCTTTCAGAATCATGTGGGCTGTTCATAATGGCACTCGACCACCACTGATCAAAAATTTACCTAAGCCCATTGAGAGCTTGATGACACGCTGTTGGTCTAAGGACCCATCTCAGCGCCCTTCAATGGAGGAAATTGTGAAAATAATGACTCACTTGATGCGGTACTTCCCAGGAGCGGATGAGCCGTTACAGTATCCTTGTCAGTACTCTGATGAAGGGCAGAGCAACTCAGCCACCAGCACAGGCTCATTCATGGACATTGCTTCTACAAATACCAGTAATAAAAGTGACACAAATATGGAACAGGTTCCTGCCACAAACGACACTATTAAACGCTTGGAGTCAAAACTTTTGAAAAACCAGGCAAAGCAACAGCTCCGGCAGTTGTCACGTGTGAACCATCCTAACATTGTCAAGTTGTATGGAGCCTGCCTGAATCCAGTATGTCTTGTGATGGAATATGCAGAGGGGGGCTCATTGTATAATGTGCTGCATGGTGCTGAACCATTGCCTTACTACACTGCTGCTCATGCCATGAGCTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTACCTGCACAGCATGCAGCCCAAAGCGCTGATTCACAGGGACCTCAAGCCTCCAAACTTGCTGCTGGTTGCAGGAGGGACAGTTCTAAAAATCTGCGATTTTGGTACAGCTTGTGACATCCAAACACACATGACCAATAATAAAGGGAGTGCTGCTTGGATGGCGCCTGAAGTATTTGAAGGTAGCAATTACAGTGAAAAGTGTGATGTCTTCAGCTGGGGTATTATCCTCTGGGAAGTGATAACACGCCGGAAACCCTTCGATGAGATCGGTGGCCCAGCTTTCAGAATCATGTGGGCTGTTCATAATGGCACTCGACCACCACTGATCAAAAATTTACCTAAGCCCATTGAGAGCTTGATGACACGCTGTTGGTCTAAGGACCCATCTCAGCGCCCTTCAATGGAGGAAATTGTGAAAATAATGACTCACTTGATGCGGTACTTCCCAGGAGCGGATGAGCCGTTACAGTATCCTTGTCAGTACTCTGATGAAGGGCAGAGCAACTCAGCCACCAGCACAGGCTCATTCATGGACATTGCTTCTACAAATACCAGTAATAAAAGTGACACAAATATGGAACAGGTTCCTGCCACAAACGACACTATTAAACGCTTGGAGTCAAAACTTTTGAAAAACCAGGCAAAGCAACAG
Peptide sequence:
MEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQLRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQ
IRESfinder result
IRESfinder score
0.371827268
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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