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mmu_circMap3k21_870
Basic information of circular RNA
PanCircBase ID:
mmu_circMap3k21_870
mm39_Chr. Position ID:
chr8|126650756|126664067|+
Gene name:
Map3k21
Transcript:
ENSMUST00000034316.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
2,3,4,5,6
Exon/intron offsets:
0,2057,3513,10911,13188
Genomic length:
13311
Exon/intron sizes in nt:
18,11,49,17,62,41,123
Flanking exon/introns:
chr8:126638172-126650756|chr8:126664067-126665439
Splice length:
870
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
TTTTGCTGCTGGAGAAGATAGAACATGATGACATTTGCAACAAGACCCTGAAGATCACGGACTTTGGGCTAGCGAGGGAATGGCACAGGACCACCAGGATGAGCGCAGCAGGGACCTACGCCTGGATGGCTCCCGAGGTCATCAGGTCCTCCCTGTTCTCCAAGGGCAGCGACATCTGGAGCTATGGAGTGCTGTTGTGGGAGCTGCTCACTGGTGAGGTCCCGTACCGTGGCATCGATGGACTGGCTGTGGCGTACGGGGTGGCTGTCAACAAGCTCACACTGCCTATTCCATCCACCTGCCCTGAACCGTTTGCAAAACTCATGAAAGAGTGCTGGGAACAAGACCCTCATATCCGCCCCTCGTTTGCCTTAATTCTTCAGCAGCTGACTGCGATCGAAGAGGCAGTGTTGACCAACATGCCCCAGGAGTCCTTCCATTCCATGCAGGAAGACTGGAAACTGGAGATTCAGCAAATGTTCAGTGAGCTGCGAACCAAGGAGAAGGAGCTGCGTTCCCGAGAGGAGGAGCTGAGCCGAGCTGCCTTGCAACAGAAATCGCAGGAACTGCTGCTCAGACGCCGCGAGCAGCAGCTCGCGGAGCGTGAGATCGACGTGCTGGAGCGCGAGCTGAACGTGCTCATCTTCCAGCTGAGCCAGGAGGCTCCCCACGTGAAGAAGAGGAAGGGAAGGTTCAGGAGAGGACGGCTGAGGCTCAAAGACGGCCACCGCATCAGCCTGCCTTCAGATTTCCAGCACAAGATCACAGTGCAGGCTTCCCCGACGCTGGACAAGCGCCGAAGCTCAGACAGCGGACTGTGCAGCCCACCCGGCAGCCCCTTGATGCTCCCCCGCCTCCGGGCTATTCAGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCCGAAGCTCAGACAG
61.18
CATTCCCTCGCTAGCCCAAA
60.11
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCGCCGAAGCTCAGACAG
61.18
GCTAGCCCAAAGTCCGTGAT
60.11
150
PCR template sequence:
CAGGCTTCCCCGACGCTGGACAAGCGCCGAAGCTCAGACAGCGGACTGTGCAGCCCACCCGGCAGCCCCTTGATGCTCCCCCGCCTCCGGGCTATTCAGTTTTTGCTGCTGGAGAAGATAGAACATGATGACATTTGCAACAAGACCCTGAAGATCACGGACTTTGGGCTAGCGAGGGAATGGCACAGGACCACCAGGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-15b-5p(2), mmu-miR-24-3p(2), mmu-miR-207(2), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-322-5p(1), mmu-miR-196a-5p(1), mmu-miR-370-3p(1), mmu-miR-34b-5p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CGGGCTATTCAGTTTTTGCTGCTGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAP3K21_870_ORF_1
1.2871
0.460460001
0.999980291
1641
ORF sequence:
ATGAGCGCAGCAGGGACCTACGCCTGGATGGCTCCCGAGGTCATCAGGTCCTCCCTGTTCTCCAAGGGCAGCGACATCTGGAGCTATGGAGTGCTGTTGTGGGAGCTGCTCACTGGTGAGGTCCCGTACCGTGGCATCGATGGACTGGCTGTGGCGTACGGGGTGGCTGTCAACAAGCTCACACTGCCTATTCCATCCACCTGCCCTGAACCGTTTGCAAAACTCATGAAAGAGTGCTGGGAACAAGACCCTCATATCCGCCCCTCGTTTGCCTTAATTCTTCAGCAGCTGACTGCGATCGAAGAGGCAGTGTTGACCAACATGCCCCAGGAGTCCTTCCATTCCATGCAGGAAGACTGGAAACTGGAGATTCAGCAAATGTTCAGTGAGCTGCGAACCAAGGAGAAGGAGCTGCGTTCCCGAGAGGAGGAGCTGAGCCGAGCTGCCTTGCAACAGAAATCGCAGGAACTGCTGCTCAGACGCCGCGAGCAGCAGCTCGCGGAGCGTGAGATCGACGTGCTGGAGCGCGAGCTGAACGTGCTCATCTTCCAGCTGAGCCAGGAGGCTCCCCACGTGAAGAAGAGGAAGGGAAGGTTCAGGAGAGGACGGCTGAGGCTCAAAGACGGCCACCGCATCAGCCTGCCTTCAGATTTCCAGCACAAGATCACAGTGCAGGCTTCCCCGACGCTGGACAAGCGCCGAAGCTCAGACAGCGGACTGTGCAGCCCACCCGGCAGCCCCTTGATGCTCCCCCGCCTCCGGGCTATTCAGTTTTTGCTGCTGGAGAAGATAGAACATGATGACATTTGCAACAAGACCCTGAAGATCACGGACTTTGGGCTAGCGAGGGAATGGCACAGGACCACCAGGATGAGCGCAGCAGGGACCTACGCCTGGATGGCTCCCGAGGTCATCAGGTCCTCCCTGTTCTCCAAGGGCAGCGACATCTGGAGCTATGGAGTGCTGTTGTGGGAGCTGCTCACTGGTGAGGTCCCGTACCGTGGCATCGATGGACTGGCTGTGGCGTACGGGGTGGCTGTCAACAAGCTCACACTGCCTATTCCATCCACCTGCCCTGAACCGTTTGCAAAACTCATGAAAGAGTGCTGGGAACAAGACCCTCATATCCGCCCCTCGTTTGCCTTAATTCTTCAGCAGCTGACTGCGATCGAAGAGGCAGTGTTGACCAACATGCCCCAGGAGTCCTTCCATTCCATGCAGGAAGACTGGAAACTGGAGATTCAGCAAATGTTCAGTGAGCTGCGAACCAAGGAGAAGGAGCTGCGTTCCCGAGAGGAGGAGCTGAGCCGAGCTGCCTTGCAACAGAAATCGCAGGAACTGCTGCTCAGACGCCGCGAGCAGCAGCTCGCGGAGCGTGAGATCGACGTGCTGGAGCGCGAGCTGAACGTGCTCATCTTCCAGCTGAGCCAGGAGGCTCCCCACGTGAAGAAGAGGAAGGGAAGGTTCAGGAGAGGACGGCTGAGGCTCAAAGACGGCCACCGCATCAGCCTGCCTTCAGATTTCCAGCACAAGATCACAGTGCAGGCTTCCCCGACGCTGGACAAGCGCCGAAGCTCAGACAGCGGACTGTGCAGCCCACCCGGCAGCCCCTTGATGCTCCCCCGCCTCCGGGCTATTCAG
Peptide sequence:
MSAAGTYAWMAPEVIRSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWEQDPHIRPSFALILQQLTAIEEAVLTNMPQESFHSMQEDWKLEIQQMFSELRTKEKELRSREEELSRAALQQKSQELLLRRREQQLAEREIDVLERELNVLIFQLSQEAPHVKKRKGRFRRGRLRLKDGHRISLPSDFQHKITVQASPTLDKRRSSDSGLCSPPGSPLMLPRLRAIQFLLLEKIEHDDICNKTLKITDFGLAREWHRTTRMSAAGTYAWMAPEVIRSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWEQDPHIRPSFALILQQLTAIEEAVLTNMPQESFHSMQEDWKLEIQQMFSELRTKEKELRSREEELSRAALQQKSQELLLRRREQQLAEREIDVLERELNVLIFQLSQEAPHVKKRKGRFRRGRLRLKDGHRISLPSDFQHKITVQASPTLDKRRSSDSGLCSPPGSPLMLPRLRAIQ
IRESfinder result
IRESfinder score
0.233606023
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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