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mmu_circMap3k15_766
Basic information of circular RNA
PanCircBase ID:
mmu_circMap3k15_766
mm39_Chr. Position ID:
chrX|158858590|158872887|+
Gene name:
Map3k15
Transcript:
ENSMUST00000033665.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
8,9,10,11,12,13,14
Exon/intron offsets:
0,534,4319,6190,8269,10723,14209
Genomic length:
14297
Exon/intron sizes in nt:
113,160,151,108,50,96,88
Flanking exon/introns:
chrX:158856004-158858590|chrX:158872887-158874576
Splice length:
766
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATCGCAAAGGGTTTGAACTCCAGTCATCCCTTTATTCAGGAATTAACCTTGCAGTTTTGCTGATAGTTTCTGGACAACAGTTTGAAACTTCGATGGAACTAAGGAAAATAGGTGTCCGGCTGAACAGTTTATTGGGAAGAAAAGGGAGCCTGGAGAAAATGAACAATTACTGGGATGTAGGTCAGTTCTTCACCGTCAGCATGCTGGCAAGTGATATTGGGAAAGCTGTCCAGGCAGCAGAGAGGTTGTTCAAACTGAAACCCCCAGTCTGGTACCTGCGGTCATTAGTTCAGAACTTGCTGTTAATTCAACGCTTCAAGAAACCCATTACAGAACATTCACCCAGGCAGGAACGGCTTAACTTCTGGTTAGATATCATTTTTGAAGCAACGAATGAAGTTACTAATGGACTCAGATTTCCAGTTCTGGTAATAGAGCCAACCAAAGTCTACCAGCCTTCTTATGTTTCTATCAACAATGAAGCTGAAGAAAGAACTGTTTCTTTATGGCATGTCTCACCCACAGAAATGAAACAAATCCATGAGTGGAATTTTACAGCCTCTTCTATTAAAGGAATAAGCCTATCCAAGTTTGATGAACGGTGCTGTTTTCTTTATGTCCATGATAATTCTGATGACTTTCAAATCTACTTTTCCACCGAAGACCAGTGTAATAGATTTTGTTCTTTGGTCAAAGAGATGCTAAACAATGGAGTGGGCAGTACAGTGGAGTTGGAGGGAGAGGCTGATGGAGACACCTTAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGTGGGCAGTACAGTGGAG
59.75
TCGAAGTTTCAAACTGTTGTCCA
59
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGCAGTACAGTGGAGTTGG
60.32
TCGAAGTTTCAAACTGTTGTCCA
59
144
PCR template sequence:
CCAGTGTAATAGATTTTGTTCTTTGGTCAAAGAGATGCTAAACAATGGAGTGGGCAGTACAGTGGAGTTGGAGGGAGAGGCTGATGGAGACACCTTAGAGGTATCGCAAAGGGTTTGAACTCCAGTCATCCCTTTATTCAGGAATTAACCTTGCAGTTTTGCTGATAGTTTCTGGACAACAGTTTGAAACTTCGATGGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-25-3p(1), mmu-miR-136-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
AGACACCTTAGAGGTATCGCAAAGGG
siRNA sets
CTTTGCGATACCTCTAAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAP3K15_766_ORF_1
0.7493
0.030374556
0.771702873
690
ORF sequence:
ATGGAACTAAGGAAAATAGGTGTCCGGCTGAACAGTTTATTGGGAAGAAAAGGGAGCCTGGAGAAAATGAACAATTACTGGGATGTAGGTCAGTTCTTCACCGTCAGCATGCTGGCAAGTGATATTGGGAAAGCTGTCCAGGCAGCAGAGAGGTTGTTCAAACTGAAACCCCCAGTCTGGTACCTGCGGTCATTAGTTCAGAACTTGCTGTTAATTCAACGCTTCAAGAAACCCATTACAGAACATTCACCCAGGCAGGAACGGCTTAACTTCTGGTTAGATATCATTTTTGAAGCAACGAATGAAGTTACTAATGGACTCAGATTTCCAGTTCTGGTAATAGAGCCAACCAAAGTCTACCAGCCTTCTTATGTTTCTATCAACAATGAAGCTGAAGAAAGAACTGTTTCTTTATGGCATGTCTCACCCACAGAAATGAAACAAATCCATGAGTGGAATTTTACAGCCTCTTCTATTAAAGGAATAAGCCTATCCAAGTTTGATGAACGGTGCTGTTTTCTTTATGTCCATGATAATTCTGATGACTTTCAAATCTACTTTTCCACCGAAGACCAGTGTAATAGATTTTGTTCTTTGGTCAAAGAGATGCTAAACAATGGAGTGGGCAGTACAGTGGAGTTGGAGGGAGAGGCTGATGGAGACACCTTAGAGGTATCGCAAAGGGTTTGA
Peptide sequence:
MELRKIGVRLNSLLGRKGSLEKMNNYWDVGQFFTVSMLASDIGKAVQAAERLFKLKPPVWYLRSLVQNLLLIQRFKKPITEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQIHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEDQCNRFCSLVKEMLNNGVGSTVELEGEADGDTLEVSQRV
IRESfinder result
IRESfinder score
0.657835936
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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