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mmu_circMap2_143
Basic information of circular RNA
PanCircBase ID:
mmu_circMap2_143
mm39_Chr. Position ID:
chr1|66361562|66401439|+
Gene name:
Map2
Transcript:
ENSMUST00000156636.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,39799
Genomic length:
39877
Exon/intron sizes in nt:
65,78
Flanking exon/introns:
chr1:66290871-66361562|chr1:66401439-66419647
Splice length:
143
Other database IDs:
circAtlas: mmu-Map2_0074
circAtlas: hsa-MAP2_0079
Human Ortholog IDs:
Mature spliced Sequence:
GTTCCTCAGCTTGTCTCTAACAGAGGAAGCACTGATTGGGAAGCACTCAGTCAGAAAATTAAAAGAAAGAAGCCAGAACATACCACCAGCCGTTTGAGAATACAACATAGCAAACTTCACTACTTTACAACTTCCTTGAATTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGAACATACCACCAGCCGT
60.04
TCTGACTGAGTGCTTCCCAA
58.58
125
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGAACATACCACCAGCCGT
60.04
TTCTGACTGAGTGCTTCCCA
58.58
126
PCR template sequence:
CCAGAACATACCACCAGCCGTTTGAGAATACAACATAGCAAACTTCACTACTTTACAACTTCCTTGAATTGGTTCCTCAGCTTGTCTCTAACAGAGGAAGCACTGATTGGGAAGCACTCAGTCAGAAAATTAAAAGAAAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-138-5p(1), mmu-miR-382-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTCCTTGAATTGGTTCCTCAGCTTG
siRNA sets
AAGCTGAGGAACCAATTCA, CAAGCTGAGGAACCAATTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.903060491
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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