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mmu_circMan2a1_751
Basic information of circular RNA
PanCircBase ID:
mmu_circMan2a1_751
mm39_Chr. Position ID:
chr17|64973731|64982242|+
Gene name:
Man2a1
Transcript:
ENSMUST00000086723.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,8,9,10
Exon/intron offsets:
0,2675,5111,8328
Genomic length:
8511
Exon/intron sizes in nt:
1,87,17,82,03,183
Flanking exon/introns:
chr17:64966069-64973731|chr17:64982242-64986746
Splice length:
751
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATCTTGGATCTGCTACAGACATTTTGTGCCATATGATGCCCTTCTACAGCTACGACATCCCTCACACCTGTGGGCCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAACGGCTTCCTGGAGGCAGATATGGTTGTCCCTGGGGAGTTCCCCCAGAAGCAATATCTCCTGGAAATGTCCAAAGCAGGGCTCAGATGCTATTGGATCAGTACCGGAAAAAGTCAAAACTTTTCCGCACTAAAGTTCTGCTGGCTCCACTGGGAGACGACTTTCGGTTCAGTGAATACACAGAGTGGGATCTGCAGTGCAGGAACTACGAGCAACTGTTCAGTTACATGAACTCGCAGCCTCATCTGAAAGTGAAGATCCAGTTTGGAACCTTGTCAGATTATTTCGACGCATTGGAGAAAGCGGTGGCAGCCGAGAAGAAGAGTAGCCAGTCTGTGTTCCCTGCCCTGAGTGGAGACTTCTTCACGTACGCTGACAGAGACGACCATTACTGGAGTGGCTACTTCACGTCCAGACCTTTCTACAAACGAATGGACAGAATAATGGAATCTCGTATAAGGGCTGCTGAAATTCTTTACCAGTTGGCCTTGAAACAAGCTCAGAAATACAAGATAAATAAATTTCTTTCATCACCTCATTACACAACACTGACAGAAGCCAGAAGGAACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACACTGACAGAAGCCAGAAGG
59.93
GGTGTGAGGGATGTCGTAGC
60.18
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGCCATCACAGGAACCG
60.39
TTTAGGATCAGGCCCACAGG
59.08
140
PCR template sequence:
CAACACTGACAGAAGCCAGAAGGAACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACCAGATCTTGGATCTGCTACAGACATTTTGTGCCATATGATGCCCTTCTACAGCTACGACATCCCTCACACCTGTGGGCCTGATCCTAAAATATGCTGCCAGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTATGGTACCAGATCTTGGATCTGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAN2A1_751_ORF_1
0.6176
0.173722361
0.915480461
804
ORF sequence:
ATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACCAGATCTTGGATCTGCTACAGACATTTTGTGCCATATGATGCCCTTCTACAGCTACGACATCCCTCACACCTGTGGGCCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAACGGCTTCCTGGAGGCAGATATGGTTGTCCCTGGGGAGTTCCCCCAGAAGCAATATCTCCTGGAAATGTCCAAAGCAGGGCTCAGATGCTATTGGATCAGTACCGGAAAAAGTCAAAACTTTTCCGCACTAAAGTTCTGCTGGCTCCACTGGGAGACGACTTTCGGTTCAGTGAATACACAGAGTGGGATCTGCAGTGCAGGAACTACGAGCAACTGTTCAGTTACATGAACTCGCAGCCTCATCTGAAAGTGAAGATCCAGTTTGGAACCTTGTCAGATTATTTCGACGCATTGGAGAAAGCGGTGGCAGCCGAGAAGAAGAGTAGCCAGTCTGTGTTCCCTGCCCTGAGTGGAGACTTCTTCACGTACGCTGACAGAGACGACCATTACTGGAGTGGCTACTTCACGTCCAGACCTTTCTACAAACGAATGGACAGAATAATGGAATCTCGTATAAGGGCTGCTGAAATTCTTTACCAGTTGGCCTTGAAACAAGCTCAGAAATACAAGATAAATAAATTTCTTTCATCACCTCATTACACAACACTGACAGAAGCCAGAAGGAACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACC
Peptide sequence:
MMPSQEPRKTGWLWTMVPDLGSATDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGT
IRESfinder result
IRESfinder score
0.597058308
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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