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mmu_circMan2a1_315
Basic information of circular RNA
PanCircBase ID:
mmu_circMan2a1_315
mm39_Chr. Position ID:
chr17|65047674|65057889|+
Gene name:
Man2a1
Transcript:
ENSMUST00000086723.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
20,21
Exon/intron offsets:
0,10104
Genomic length:
10215
Exon/intron sizes in nt:
2,04,111
Flanking exon/introns:
chr17:65042062-65047674|chr17:65057889-65059408
Splice length:
315
Other database IDs:
circAtlas: mmu-Man2a1_0035
Human Ortholog IDs:
Mature spliced Sequence:
GAAGAAGAAAAGAAGAGCCCTGTCAGCTACCCTTCCCTCCTCAGCCACATGACTTCGTCCTTCCTCAACCATCCCTTTCTCCCCATGGTACTAAGTGGCCAGCTCCCCTCCCCTGCCTTTGAGCTGCTGAGTGAATTTCCTCTGCTGCAGTCCTCTCTACCTTGTGATATCCATCTGGTCAACCTGCGGACAATACAATCAAAGATGGGCAAAGGCTATTCGGATGAGGCAGCCTTGATCCTCCACAGGAAAGGGTTTGATTGCCAGTTCTCCAGCAGAGGCATCGGGCTACCCTGTTCCACTACTCAGGGAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCCTTGATCCTCCACAGG
60.11
ACGAAGTCATGTGGCTGAGG
60.04
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCCTTGATCCTCCACAGG
60.11
GGGATGGTTGAGGAAGGACG
60.11
161
PCR template sequence:
CTATTCGGATGAGGCAGCCTTGATCCTCCACAGGAAAGGGTTTGATTGCCAGTTCTCCAGCAGAGGCATCGGGCTACCCTGTTCCACTACTCAGGGAAAGGAAGAAGAAAAGAAGAGCCCTGTCAGCTACCCTTCCCTCCTCAGCCACATGACTTCGTCCTTCCTCAACCATCCCTTTCTCCCCATGGTACTAAGTGGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-298-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TACTCAGGGAAAGGAAGAAGAAAAGA
siRNA sets
TCTTTTCTTCTTCCTTTCC, TTTTCTTCTTCCTTTCCCT, TTTCTTCTTCCTTTCCCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAN2A1_315_ORF_1
0.7605
0.169521451
0.740658765
582
ORF sequence:
ATGACTTCGTCCTTCCTCAACCATCCCTTTCTCCCCATGGTACTAAGTGGCCAGCTCCCCTCCCCTGCCTTTGAGCTGCTGAGTGAATTTCCTCTGCTGCAGTCCTCTCTACCTTGTGATATCCATCTGGTCAACCTGCGGACAATACAATCAAAGATGGGCAAAGGCTATTCGGATGAGGCAGCCTTGATCCTCCACAGGAAAGGGTTTGATTGCCAGTTCTCCAGCAGAGGCATCGGGCTACCCTGTTCCACTACTCAGGGAAAGGAAGAAGAAAAGAAGAGCCCTGTCAGCTACCCTTCCCTCCTCAGCCACATGACTTCGTCCTTCCTCAACCATCCCTTTCTCCCCATGGTACTAAGTGGCCAGCTCCCCTCCCCTGCCTTTGAGCTGCTGAGTGAATTTCCTCTGCTGCAGTCCTCTCTACCTTGTGATATCCATCTGGTCAACCTGCGGACAATACAATCAAAGATGGGCAAAGGCTATTCGGATGAGGCAGCCTTGATCCTCCACAGGAAAGGGTTTGATTGCCAGTTCTCCAGCAGAGGCATCGGGCTACCCTGTTCCACTACTCAGGGAAAG
Peptide sequence:
MTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDIHLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDIHLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGK
IRESfinder result
IRESfinder score
0.564475277
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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