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mmu_circMan2a1_172
Basic information of circular RNA
PanCircBase ID:
mmu_circMan2a1_172
mm39_Chr. Position ID:
chr17|64943478|64943650|+
Gene name:
Man2a1
Transcript:
ENSMUST00000086723.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
172
Exon/intron sizes in nt:
172
Flanking exon/introns:
chr17:64932446-64943478|chr17:64943650-64958190
Splice length:
172
Other database IDs:
circBase: hsa_circ_0009174, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_49802
Human Ortholog IDs:
Mature spliced Sequence:
GTTGGTTGAAGACTTTCAATGACTACTTTAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTTATGTGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATATTCCGAAGAAGGAAGCTGTTAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTTGCAAAATGGTGGGAT
57.97
AGTCTTCTTTCAGCTTTAGGACCA
59.65
139
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTTGCAAAATGGTGGGAT
57.97
TCTTTCAGCTTTAGGACCATGT
57.36
134
PCR template sequence:
AGCAGGAAGTTTATGTGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATATTCCGAAGAAGGAAGCTGTTAAAAGGTTGGTTGAAGACTTTCAATGACTACTTTAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AAGCTGTTAAAAGGTTGGTTGAAGAC
siRNA sets
CAACCAACCTTTTAACAGC, TCAACCAACCTTTTAACAG, CTTCAACCAACCTTTTAAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAN2A1_172_ORF_1
0.7268
-0.023389375
0.046485552
135
ORF sequence:
ATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTTATGTGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATATTCCGAAGAAGGAAGCTGTTAAAAGGTTGGTTGAAGACTTTCAATGA
Peptide sequence:
MVLKLKEDSSRKFMWSEISYLAKWWDIIDIPKKEAVKRLVEDFQ
IRESfinder result
IRESfinder score
0.702181687
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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