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mmu_circMan2a1_1225
Basic information of circular RNA
PanCircBase ID:
mmu_circMan2a1_1225
mm39_Chr. Position ID:
chr17|64943478|64982242|+
Gene name:
Man2a1
Transcript:
ENSMUST00000086723.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
4,5,6,7,8,9,10
Exon/intron offsets:
0,14712,22417,30253,32928,35364,38581
Genomic length:
38764
Exon/intron sizes in nt:
17,21,28,17,41,87,17,80,00,000
Flanking exon/introns:
chr17:64932446-64943478|chr17:64982242-64986746
Splice length:
1225
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_147216
Human Ortholog IDs:
Mature spliced Sequence:
GTTGGTTGAAGACTTTCAATGACTACTTTAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTTATGTGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATATTCCGAAGAAGGAAGCTGTTAAAAGTTTACTACAGAATGGTCAGCTGGAAATTGTGACCGGTGGCTGGGTTATGCCTGATGAAGCCACTCCACATTATTTTGCCTTAATTGACCAACTAATTGAAGGGCACCAATGGCTGGAAAAAAATCTAGGAGTGAAACCTCGATCGGGCTGGGCCATAGATCCCTTTGGTCATTCACCCACAATGGCTTATCTTCTAAAGCGTGCTGGATTTTCACACATGCTCATCCAGAGAGTCCATTATGCAATCAAAAAACACTTCTCTTTGCATAAAACGCTGGAGTTTTTCTGGAGACAGAATTGGGATCTTGGATCTGCTACAGACATTTTGTGCCATATGATGCCCTTCTACAGCTACGACATCCCTCACACCTGTGGGCCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAACGGCTTCCTGGAGGCAGATATGGTTGTCCCTGGGGAGTTCCCCCAGAAGCAATATCTCCTGGAAATGTCCAAAGCAGGGCTCAGATGCTATTGGATCAGTACCGGAAAAAGTCAAAACTTTTCCGCACTAAAGTTCTGCTGGCTCCACTGGGAGACGACTTTCGGTTCAGTGAATACACAGAGTGGGATCTGCAGTGCAGGAACTACGAGCAACTGTTCAGTTACATGAACTCGCAGCCTCATCTGAAAGTGAAGATCCAGTTTGGAACCTTGTCAGATTATTTCGACGCATTGGAGAAAGCGGTGGCAGCCGAGAAGAAGAGTAGCCAGTCTGTGTTCCCTGCCCTGAGTGGAGACTTCTTCACGTACGCTGACAGAGACGACCATTACTGGAGTGGCTACTTCACGTCCAGACCTTTCTACAAACGAATGGACAGAATAATGGAATCTCGTATAAGGGCTGCTGAAATTCTTTACCAGTTGGCCTTGAAACAAGCTCAGAAATACAAGATAAATAAATTTCTTTCATCACCTCATTACACAACACTGACAGAAGCCAGAAGGAACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGCCATCACAGGAACCG
60.39
CCTGCTTGAGTCTTCTTTCAGC
59.52
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CATCACAGGAACCGCGAAAG
59.56
CCTGCTTGAGTCTTCTTTCAGC
59.52
140
PCR template sequence:
CAACACTGACAGAAGCCAGAAGGAACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACCAGGTTGGTTGAAGACTTTCAATGACTACTTTAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTTAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTATGGTACCAGGTTGGTTGAAGAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMAN2A1_1225_ORF_1
0.6832
0.112122007
0.996498312
1278
ORF sequence:
ATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACCAGGTTGGTTGAAGACTTTCAATGACTACTTTAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTTATGTGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATATTCCGAAGAAGGAAGCTGTTAAAAGTTTACTACAGAATGGTCAGCTGGAAATTGTGACCGGTGGCTGGGTTATGCCTGATGAAGCCACTCCACATTATTTTGCCTTAATTGACCAACTAATTGAAGGGCACCAATGGCTGGAAAAAAATCTAGGAGTGAAACCTCGATCGGGCTGGGCCATAGATCCCTTTGGTCATTCACCCACAATGGCTTATCTTCTAAAGCGTGCTGGATTTTCACACATGCTCATCCAGAGAGTCCATTATGCAATCAAAAAACACTTCTCTTTGCATAAAACGCTGGAGTTTTTCTGGAGACAGAATTGGGATCTTGGATCTGCTACAGACATTTTGTGCCATATGATGCCCTTCTACAGCTACGACATCCCTCACACCTGTGGGCCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAACGGCTTCCTGGAGGCAGATATGGTTGTCCCTGGGGAGTTCCCCCAGAAGCAATATCTCCTGGAAATGTCCAAAGCAGGGCTCAGATGCTATTGGATCAGTACCGGAAAAAGTCAAAACTTTTCCGCACTAAAGTTCTGCTGGCTCCACTGGGAGACGACTTTCGGTTCAGTGAATACACAGAGTGGGATCTGCAGTGCAGGAACTACGAGCAACTGTTCAGTTACATGAACTCGCAGCCTCATCTGAAAGTGAAGATCCAGTTTGGAACCTTGTCAGATTATTTCGACGCATTGGAGAAAGCGGTGGCAGCCGAGAAGAAGAGTAGCCAGTCTGTGTTCCCTGCCCTGAGTGGAGACTTCTTCACGTACGCTGACAGAGACGACCATTACTGGAGTGGCTACTTCACGTCCAGACCTTTCTACAAACGAATGGACAGAATAATGGAATCTCGTATAAGGGCTGCTGAAATTCTTTACCAGTTGGCCTTGAAACAAGCTCAGAAATACAAGATAAATAAATTTCTTTCATCACCTCATTACACAACACTGACAGAAGCCAGAAGGAACTTAGGACTATTTCAGCATCATGATGCCATCACAGGAACCGCGAAAGACTGGGTGGTTGTGGACTATGGTACC
Peptide sequence:
MMPSQEPRKTGWLWTMVPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSSRKFMWSEISYLAKWWDIIDIPKKEAVKSLLQNGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDDFRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGT
IRESfinder result
IRESfinder score
0.614153321
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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