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mmu_circLyst_2905
Basic information of circular RNA
PanCircBase ID:
mmu_circLyst_2905
mm39_Chr. Position ID:
chr13|13824908|13856107|+
Gene name:
Lyst
Transcript:
ENSMUST00000110559.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
13,14,15,16,17,18,19,20,21,22,23,24,25,26
Exon/intron offsets:
0,1253,6510,8273,10604,13616,19516,20479,22932,24006,27325,28639,29749,30968
Genomic length:
31199
Exon/intron sizes in nt:
1,45,17,41,61,19,12,46,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr13:13823551-13824908|chr13:13856107-13857639
Splice length:
2905
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAGGTGATAGACCAGAAGTTACAGAATCCATCAATCCTGGTGACAGACTCATAGAAGACGGCTGTATTCATTTGATTTCACTGGGGTCCAAAGCATTGATGATCCAAGTGTGGGCTGATCCCCACAGTGGCACTTTTATCTTTCGTGTGTGCATGGACTCAAATGATGACACGAAAGCTGTCTCACTAGCACAGGTGGAATCACAGGAGAATATTTTCTTTCCAAGCAAATGGCAACACTTAGTACTTACCTATATTCAGCATCCTCAAGGGAAAAAGAATGTCCATGGGGAAATCTCCATATGGGTCTCTGGGCAGAGGAAGACTGATGTCATCTTGGATTTTGTGCTCCCAAGAAAAACAAGCTTATCATCAGACAGCAATAAAACATTTTGCATGATTGGTCATTGCTTAACATCCCAAGAAGAGTCTCTGCAATTAGCTGGAAAATGGGACCTGGGGAACTTGCTCCTCTTCAATGGAGCTAAAATTGGCTCACAAGAGGCCTTTTTCCTGTATGCTTGTGGACCCAACTACACATCCATCATGCCGTGTAAATATGGACAGCCAGTCATTGACTACTCCAAATACATTAATAAAGACATTTTGAGATGTGATGAAATCAGAGACCTTTTTATGACCAAGAAAGAAGTGGATGTTGGTCTCTTAATTGAAAGTCTTTCAGTTGTTTATACAACTTGCTGTCCTGCTCAGTACACCATCTATGAACCAGTGATTCGACTCAAGGGCCAAGTGAAAACTCAGCCCTCTCAAAGACCCTTCAGCTCAAAGGAAGCCCAGAGCATCTTGCTAGAACCTTCTCAACTCAAAGGCCTCCAACCTACGGAATGTAAAGCCATCCAGGGCATTCTGCATGAGATTGGTGGGGCTGGCACATTTGTTTTTCTCTTTGCTAGGGTTGTTGAACTTAGTAGCTGTGAAGAAACTCAAGCATTAGCACTGCGGGTTATACTGTCTTTAATTAAGTACAGCCAACAGAGAACACAGGAACTGGAAAATTGTAATGGACTCTCTATGATTCACCAAGTGTTGGTCAAACAGAAATGCATTGTTGGCTTTCACATTTTGAAGACCCTTCTTGAAGGTTGCTGCGGTGAAGAAGTTATCCACGTCAGTGAGCATGGAGAGTTCAAGCTGGATGTTGAGTCTCATGCTATAATCCAAGATGTTAAGCTGCTGCAGGAACTGTTACTTGACTGGAAGATATGGAATAAGGCAGAGCAAGGTGTGTGGGAGACTCTGCTAGCAGCTTTGGAAGTCCTCATCCGGGTAGAGCACCACCAGCAGCAGTTTAATATTAAGCAGTTGCTGAACGCCCACGTGGTTCACCACTTCCTACTGACCTGTCAGGTTTTACAGGAACACAGAGAGGGGCAGCTTACATCTATGCCCCGAGAAGTTTGTAGATCATTTGTGAAAATCATTGCAGAAGTCCTTGGTTCTCCTCCAGACTTGGAATTATTGACAGTTATTTTCAATTTCCTGTTAGCTGTACACCCTCCTACTAATACTTATGTTTGTCACAATCCCACAAACTTCTACTTCTCTTTGCACATAGATGGCAAGATCTTTCAGGAGAAAGTGCAGTCACTCGCGTACCTGAGGCATTCTAGCAGCGGAGGGCAAGCCTTTCCCAGCCCTGGATTCCTGGTAATAAGCCCATCTGCCTTTACTGCAGCTCCTCCTGAAGGAACCAGTTCTTCCAATATTGTTCCACAGCGGATGGCTGCTCAGATGGTTCGATCTAGAAGTCTACCAGCATTTCCTACTTATTTACCACTAATACGAGCACAAAAACTGGCTGCAAGTTTGGGTTTTAGTGTTGACAAGTTACAAAATATTGCAGATGCCAACCCAGAGAAACAGAATCTTTTAGGAAGACCCTACGCACTGAAAACAAGCAAAGAGGAAGCATTCATCAGCAGCTGTGAGTCTGCAAAGACTGTTTGTGAAATGGAGGCTCTTCTTGGAGCCCACGCCTCTGCCAATGGGGTTTCCAGAGGATCACCGAGGTTCCCCAGGGCCAGAGTAGATCACAAAGATGTGGGAACAGAGCCCAGATCAGATGATGACAGTCCTGGGGATGAGTCTTACCCACGTCGGCCTGACAACCTCAAGGGACTGGCCTCATTCCAGCGAAGCCAAAGCACTGTCGCAAGCCTTGGGCTGGCGTTTCCCTCTCAGAATGGATCTGCAGTTGCTAGCAGGTGGCCAAGTCTTGTTGATAGGAATGCTGATGACTGGGAGAACTTTACCTTTTCTCCTGCTTATGAGGCAAGCTACAACCGAGCCACAAGCACCCACAGTGTCATTGAAGACTGTCTGATACCTATCTGCTGTGGATTATATGAACTCTTAAGTGGGGTTCTTCTTGTCCTGCCTGATGCTATGCTTGAAGATGTGATGGACAGGATTATTCAAGCAGATATTCTTCTAGTCCTTGTTAACCACCCATCACCTGCTATCCAGCAAGGAGTAATTAAACTGTTACATGCATACATTAATAGAGCATCAAAGGAGCAAAAGGACAAGTTTCTGAAGAACCGTGGCTTTTCCTTATTAGCCAACCAGTTGTATCTTCATAGGGGAACTCAGGAGTTGTTGGAGTGCTTTGTTGAAATGTTCTTTGGTCGACCGATTGGCCTGGATGAAGAATTTGATCTGGAGGAAGTGAAGCACATGGAACTGTTCCAGAAGTGGTCTGTCATTCCCGTTCTCGGACTAATAGAGACCTCTCTCTATGACAATGTCCTCTTGCACAATGCTCTTTTACTTCTTCTGCAAGTTTTAAACTCTTGTTCCAAGGTAGCAGACATGCTACTGGACAATGGTCTACTCTATGTATTATGTAATACAGTAGCAGCCCTGAATGGATTAGAAAAGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACATGCTACTGGACAATGGTCT
59.43
TGCTTTGGACCCCAGTGAAA
59.74
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGACATGCTACTGGACAATGGT
59.43
TGCTTTGGACCCCAGTGAAA
59.74
170
PCR template sequence:
TTTTAAACTCTTGTTCCAAGGTAGCAGACATGCTACTGGACAATGGTCTACTCTATGTATTATGTAATACAGTAGCAGCCCTGAATGGATTAGAAAAGAAAAGGTGATAGACCAGAAGTTACAGAATCCATCAATCCTGGTGACAGACTCATAGAAGACGGCTGTATTCATTTGATTTCACTGGGGTCCAAAGCATTGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
28
Interacting miRNAs (#binding sites)
mmu-miR-673-5p(2), mmu-miR-137-3p(1), mmu-miR-20b-5p(1), mmu-miR-134-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-135a-5p(1), mmu-miR-205-5p(1), mmu-miR-106b-5p(1), mmu-miR-326-3p(1), mmu-miR-138-5p(1), mmu-miR-212-3p(1), mmu-miR-182-5p(1), mmu-miR-301a-3p(1), mmu-miR-499-5p(1), mmu-miR-680(1), mmu-miR-369-3p(1), mmu-miR-335-3p(1), mmu-miR-188-3p(1), mmu-miR-350-3p(1), mmu-miR-296-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GATTAGAAAAGAAAAGGTGATAGACC
siRNA sets
TATCACCTTTTCTTTTCTA,CTATCACCTTTTCTTTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCLYST_2905_ORF_1
0.8291
0.055356927
0.999999944
2814
ORF sequence:
ATGATCCAAGTGTGGGCTGATCCCCACAGTGGCACTTTTATCTTTCGTGTGTGCATGGACTCAAATGATGACACGAAAGCTGTCTCACTAGCACAGGTGGAATCACAGGAGAATATTTTCTTTCCAAGCAAATGGCAACACTTAGTACTTACCTATATTCAGCATCCTCAAGGGAAAAAGAATGTCCATGGGGAAATCTCCATATGGGTCTCTGGGCAGAGGAAGACTGATGTCATCTTGGATTTTGTGCTCCCAAGAAAAACAAGCTTATCATCAGACAGCAATAAAACATTTTGCATGATTGGTCATTGCTTAACATCCCAAGAAGAGTCTCTGCAATTAGCTGGAAAATGGGACCTGGGGAACTTGCTCCTCTTCAATGGAGCTAAAATTGGCTCACAAGAGGCCTTTTTCCTGTATGCTTGTGGACCCAACTACACATCCATCATGCCGTGTAAATATGGACAGCCAGTCATTGACTACTCCAAATACATTAATAAAGACATTTTGAGATGTGATGAAATCAGAGACCTTTTTATGACCAAGAAAGAAGTGGATGTTGGTCTCTTAATTGAAAGTCTTTCAGTTGTTTATACAACTTGCTGTCCTGCTCAGTACACCATCTATGAACCAGTGATTCGACTCAAGGGCCAAGTGAAAACTCAGCCCTCTCAAAGACCCTTCAGCTCAAAGGAAGCCCAGAGCATCTTGCTAGAACCTTCTCAACTCAAAGGCCTCCAACCTACGGAATGTAAAGCCATCCAGGGCATTCTGCATGAGATTGGTGGGGCTGGCACATTTGTTTTTCTCTTTGCTAGGGTTGTTGAACTTAGTAGCTGTGAAGAAACTCAAGCATTAGCACTGCGGGTTATACTGTCTTTAATTAAGTACAGCCAACAGAGAACACAGGAACTGGAAAATTGTAATGGACTCTCTATGATTCACCAAGTGTTGGTCAAACAGAAATGCATTGTTGGCTTTCACATTTTGAAGACCCTTCTTGAAGGTTGCTGCGGTGAAGAAGTTATCCACGTCAGTGAGCATGGAGAGTTCAAGCTGGATGTTGAGTCTCATGCTATAATCCAAGATGTTAAGCTGCTGCAGGAACTGTTACTTGACTGGAAGATATGGAATAAGGCAGAGCAAGGTGTGTGGGAGACTCTGCTAGCAGCTTTGGAAGTCCTCATCCGGGTAGAGCACCACCAGCAGCAGTTTAATATTAAGCAGTTGCTGAACGCCCACGTGGTTCACCACTTCCTACTGACCTGTCAGGTTTTACAGGAACACAGAGAGGGGCAGCTTACATCTATGCCCCGAGAAGTTTGTAGATCATTTGTGAAAATCATTGCAGAAGTCCTTGGTTCTCCTCCAGACTTGGAATTATTGACAGTTATTTTCAATTTCCTGTTAGCTGTACACCCTCCTACTAATACTTATGTTTGTCACAATCCCACAAACTTCTACTTCTCTTTGCACATAGATGGCAAGATCTTTCAGGAGAAAGTGCAGTCACTCGCGTACCTGAGGCATTCTAGCAGCGGAGGGCAAGCCTTTCCCAGCCCTGGATTCCTGGTAATAAGCCCATCTGCCTTTACTGCAGCTCCTCCTGAAGGAACCAGTTCTTCCAATATTGTTCCACAGCGGATGGCTGCTCAGATGGTTCGATCTAGAAGTCTACCAGCATTTCCTACTTATTTACCACTAATACGAGCACAAAAACTGGCTGCAAGTTTGGGTTTTAGTGTTGACAAGTTACAAAATATTGCAGATGCCAACCCAGAGAAACAGAATCTTTTAGGAAGACCCTACGCACTGAAAACAAGCAAAGAGGAAGCATTCATCAGCAGCTGTGAGTCTGCAAAGACTGTTTGTGAAATGGAGGCTCTTCTTGGAGCCCACGCCTCTGCCAATGGGGTTTCCAGAGGATCACCGAGGTTCCCCAGGGCCAGAGTAGATCACAAAGATGTGGGAACAGAGCCCAGATCAGATGATGACAGTCCTGGGGATGAGTCTTACCCACGTCGGCCTGACAACCTCAAGGGACTGGCCTCATTCCAGCGAAGCCAAAGCACTGTCGCAAGCCTTGGGCTGGCGTTTCCCTCTCAGAATGGATCTGCAGTTGCTAGCAGGTGGCCAAGTCTTGTTGATAGGAATGCTGATGACTGGGAGAACTTTACCTTTTCTCCTGCTTATGAGGCAAGCTACAACCGAGCCACAAGCACCCACAGTGTCATTGAAGACTGTCTGATACCTATCTGCTGTGGATTATATGAACTCTTAAGTGGGGTTCTTCTTGTCCTGCCTGATGCTATGCTTGAAGATGTGATGGACAGGATTATTCAAGCAGATATTCTTCTAGTCCTTGTTAACCACCCATCACCTGCTATCCAGCAAGGAGTAATTAAACTGTTACATGCATACATTAATAGAGCATCAAAGGAGCAAAAGGACAAGTTTCTGAAGAACCGTGGCTTTTCCTTATTAGCCAACCAGTTGTATCTTCATAGGGGAACTCAGGAGTTGTTGGAGTGCTTTGTTGAAATGTTCTTTGGTCGACCGATTGGCCTGGATGAAGAATTTGATCTGGAGGAAGTGAAGCACATGGAACTGTTCCAGAAGTGGTCTGTCATTCCCGTTCTCGGACTAATAGAGACCTCTCTCTATGACAATGTCCTCTTGCACAATGCTCTTTTACTTCTTCTGCAAGTTTTAAACTCTTGTTCCAAGGTAGCAGACATGCTACTGGACAATGGTCTACTCTATGTATTATGTAATACAGTAGCAGCCCTGAATGGATTAGAAAAGAAAAGGTGA
Peptide sequence:
MIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEESLQLAGKWDLGNLLLFNGAKIGSQEAFFLYACGPNYTSIMPCKYGQPVIDYSKYINKDILRCDEIRDLFMTKKEVDVGLLIESLSVVYTTCCPAQYTIYEPVIRLKGQVKTQPSQRPFSSKEAQSILLEPSQLKGLQPTECKAIQGILHEIGGAGTFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTQELENCNGLSMIHQVLVKQKCIVGFHILKTLLEGCCGEEVIHVSEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHHQQQFNIKQLLNAHVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLTVIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSLAYLRHSSSGGQAFPSPGFLVISPSAFTAAPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYLPLIRAQKLAASLGFSVDKLQNIADANPEKQNLLGRPYALKTSKEEAFISSCESAKTVCEMEALLGAHASANGVSRGSPRFPRARVDHKDVGTEPRSDDDSPGDESYPRRPDNLKGLASFQRSQSTVASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEASYNRATSTHSVIEDCLIPICCGLYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLHAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFVEMFFGRPIGLDEEFDLEEVKHMELFQKWSVIPVLGLIETSLYDNVLLHNALLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKKR
IRESfinder result
IRESfinder score
0.690514152
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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