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mmu_circLyst_2478
Basic information of circular RNA
PanCircBase ID:
mmu_circLyst_2478
mm39_Chr. Position ID:
chr13|13811952|13826335|+
Gene name:
Lyst
Transcript:
ENSMUST00000110559.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
6,7,8,9,10,11,12,13,14
Exon/intron offsets:
0,2666,3699,6070,6697,9660,11172,12956,14209
Genomic length:
14383
Exon/intron sizes in nt:
10,09,16,21,57,22,76,70,00,00,00,00,000
Flanking exon/introns:
chr13:13810694-13811952|chr13:13826335-13831418
Splice length:
2478
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGTAATAAGAGATTTGTTTTTAAGTTGTAATGGAGTAAACCAAATAATTGAACTAAATTACTTAGATGGGATTCGAAGTCATTCCCTGAAAGCATTTGAAACTCTGATTGTCAGCCTAGGGGAACAACAGAAAGATGCTGCAGTTCTAGACGTCGATGGGTTAGACATCCAACAGGAGTTGCCGTCCTTAAGTGTGGGTCCTTCTCTTCATAAGCAGCAAGCTTCTTCAGATTCTCCTTGCAGTCTCAGGAAGTTTTATGCCAGCCTCAGAGAGCCTGATCCAAAAAAACGAAAGACCATTCACCAGGATGTTCACATAAACACCATAAACCTCTTCCTCTGTGTGGCTTTTCTATGTGTCAGTAAAGAAGCAGACTCTGATAGGGAGTCTGCCAATGAGTCAGAAGATACTTCTGGCTATGACAGCACTGCCAGTGAGCCATTAAGTCACATGCTACCATGTCTGTCTCTTGAGAACGTTGTCTTACCTTCCCCTGAATGTTTGCACCATGCAGCAGACATTTGGTCCATGTGTCGTTGGATCTACATGTTGAACTCAGTCTTCCAGAAACAATTTCACAGGCTTGGTGGTTTCCAAGTGTGCCATGAATTAATATTTATGATAATCCAGAAACTATTCAGAAGTCATACAGAGGATCAAGGAAGAAGGCAGGGAGAAATGAGTAGAAATGAAAACCAAGAGCTAATCAGGATATCTTACCCCGAGCTGACACTGAAGGGAGATGTATCATCTGCAACAGCACCAGACCTGGGATTTCTGAGAAAGAGTGCTGACAGCGTGCGTGGATTCCAGTCACAGCCTGTGCTTCCCACAAGTGCAGAGCAGATTGTGGCTACTGAATCTGTTCCTGGGGAACGAAAGGCATTTATGAGTCAACAAAGTGAGACTTCTCTCCAGAGCATACGACTTTTGGAGTCTCTCCTGGCCATTTGTCTTCATAGTGCCAGAGCCTGTCAACAGAAGATGGAATTGGAGCTACCGTCTCAGGGCTTGTCTGTGGAAAATATATTGTGTGAACTGAGGGAACACCTTTCCCAGTCAAAGGTGGCAGAAACAGAATTAGCAAAGCCTTTATTTGATGCCCTGCTTCGAGTAGCCCTGGGGAATCATTCAGCAGATTTGGGCCCTGGTGATGCTGTGACTGAGAAGAGTCATCCCTCTGAGGAAGAGCTGTTGTCCCAGCCCGGAGATTTTTCAGAAGAAGCTGAGGATTCTCAGTGTTGTAGTTTGAAACTTCTGGGTGAGGAAGAAGGCTATGAAGCGGATAGTGAAAGCAATCCTGAGGATGTTGACACCCAAGACGATGGAGTAGAATTAAATCCTGAAGCAGAAGGTTTCAGTGGATCGATTGTTTCAAACAACTTACTTGAAAACCTCACTCACGGGGAAATAATATACCCTGAGATTTGCATGCTGGGATTAAATTTGCTTTCTGCTAGCAAAGCTAAACTTGATGTGCTTGCTCATGTGTTTGAGAGCTTTCTGAAAATTGTCAGGCAGAAGGAAAAGAACATTTCTCTCCTCATACAACAGGGAACTGTGAAAATCCTTCTAGGCGGGTTCTTGAATATTTTAACACAAACTAACTCTGATTTCCAAGCATGCCAGAGAGTACTGGTGGATCTCTTGGTATCTTTGATGAGCTCAAGAACGTGTTCAGAAGACTTAACTCTTCTTTTGAGAATATTTCTGGAGAAATCTCCTTGTACAGAAATTCTTCTCCTTGGTATTCACAAAATTGTTGAAAGTGATTTTACTATGAGCCCTTCACAGTGTCTGACCTTTCCTTTCCTGCATACCCCGAGTTTAAGCAATGGTGTCTTATCACAGAAACCTCCTGGGATTCTTAACAGTAAAGCCTTAGGCTTATTGAGAAGAGCACGGATTTCCCGAGGCAAGAAAGAGGCTGATAGAGAGAGTTTTCCCTATAGGCTGCTTTCCTCTTGGCATATAGCCCCAATCCACCTGCCGTTGCTGGGACAGAACTGCTGGCCACACCTGTCAGAAGGATTTAGTGTTTCTCTGTGGTTTAATGTGGAATACATCCATGAATCCGAGAGTGCTGCAGAAAGGGGAAAAAGAGTAAAGAAAAGAAACAAACCATCAGTTCTGGAAGACAGCAGTTTTGAAGGAGCAGAAGGTGATAGACCAGAAGTTACAGAATCCATCAATCCTGGTGACAGACTCATAGAAGACGGCTGTATTCATTTGATTTCACTGGGGTCCAAAGCATTGATGATCCAAGTGTGGGCTGATCCCCACAGTGGCACTTTTATCTTTCGTGTGTGCATGGACTCAAATGATGACACGAAAGCTGTCTCACTAGCACAGGTGGAATCACAGGAGAATATTTTCTTTCCAAGCAAATGGCAACACTTAGTACTTACCTATATTCAGCATCCTCAAGGGAAAAAGAATGTCCATGGGGAAATCTCCATATGGGTCTCTGGGCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAAAAAGAATGTCCATGGGG
59.5
TGCTTTCAGGGAATGACTTCGA
59.96
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAAAAAGAATGTCCATGGGG
59.5
TGCTTTCAGGGAATGACTTCG
58.57
144
PCR template sequence:
TTCCAAGCAAATGGCAACACTTAGTACTTACCTATATTCAGCATCCTCAAGGGAAAAAGAATGTCCATGGGGAAATCTCCATATGGGTCTCTGGGCAGAGGGTAATAAGAGATTTGTTTTTAAGTTGTAATGGAGTAAACCAAATAATTGAACTAAATTACTTAGATGGGATTCGAAGTCATTCCCTGAAAGCATTTGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-382-5p(2), mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-106b-5p(1), mmu-miR-216a-5p(1), mmu-miR-7a-5p(1), mmu-miR-125b-5p(1), mmu-miR-185-5p(1), mmu-miR-136-5p(1), mmu-miR-125a-5p(1), mmu-miR-183-5p(1), mmu-miR-539-5p(1), mmu-miR-335-3p(1), mmu-miR-697(1), mmu-miR-1904(1), mmu-miR-3105-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTCTGGGCAGAGGGTAATAAGAGAT
siRNA sets
TTATTACCCTCTGCCCAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCLYST_2478_ORF_1
0.8163
0.003398994
1
4503
ORF sequence:
ATGCTACCATGTCTGTCTCTTGAGAACGTTGTCTTACCTTCCCCTGAATGTTTGCACCATGCAGCAGACATTTGGTCCATGTGTCGTTGGATCTACATGTTGAACTCAGTCTTCCAGAAACAATTTCACAGGCTTGGTGGTTTCCAAGTGTGCCATGAATTAATATTTATGATAATCCAGAAACTATTCAGAAGTCATACAGAGGATCAAGGAAGAAGGCAGGGAGAAATGAGTAGAAATGAAAACCAAGAGCTAATCAGGATATCTTACCCCGAGCTGACACTGAAGGGAGATGTATCATCTGCAACAGCACCAGACCTGGGATTTCTGAGAAAGAGTGCTGACAGCGTGCGTGGATTCCAGTCACAGCCTGTGCTTCCCACAAGTGCAGAGCAGATTGTGGCTACTGAATCTGTTCCTGGGGAACGAAAGGCATTTATGAGTCAACAAAGTGAGACTTCTCTCCAGAGCATACGACTTTTGGAGTCTCTCCTGGCCATTTGTCTTCATAGTGCCAGAGCCTGTCAACAGAAGATGGAATTGGAGCTACCGTCTCAGGGCTTGTCTGTGGAAAATATATTGTGTGAACTGAGGGAACACCTTTCCCAGTCAAAGGTGGCAGAAACAGAATTAGCAAAGCCTTTATTTGATGCCCTGCTTCGAGTAGCCCTGGGGAATCATTCAGCAGATTTGGGCCCTGGTGATGCTGTGACTGAGAAGAGTCATCCCTCTGAGGAAGAGCTGTTGTCCCAGCCCGGAGATTTTTCAGAAGAAGCTGAGGATTCTCAGTGTTGTAGTTTGAAACTTCTGGGTGAGGAAGAAGGCTATGAAGCGGATAGTGAAAGCAATCCTGAGGATGTTGACACCCAAGACGATGGAGTAGAATTAAATCCTGAAGCAGAAGGTTTCAGTGGATCGATTGTTTCAAACAACTTACTTGAAAACCTCACTCACGGGGAAATAATATACCCTGAGATTTGCATGCTGGGATTAAATTTGCTTTCTGCTAGCAAAGCTAAACTTGATGTGCTTGCTCATGTGTTTGAGAGCTTTCTGAAAATTGTCAGGCAGAAGGAAAAGAACATTTCTCTCCTCATACAACAGGGAACTGTGAAAATCCTTCTAGGCGGGTTCTTGAATATTTTAACACAAACTAACTCTGATTTCCAAGCATGCCAGAGAGTACTGGTGGATCTCTTGGTATCTTTGATGAGCTCAAGAACGTGTTCAGAAGACTTAACTCTTCTTTTGAGAATATTTCTGGAGAAATCTCCTTGTACAGAAATTCTTCTCCTTGGTATTCACAAAATTGTTGAAAGTGATTTTACTATGAGCCCTTCACAGTGTCTGACCTTTCCTTTCCTGCATACCCCGAGTTTAAGCAATGGTGTCTTATCACAGAAACCTCCTGGGATTCTTAACAGTAAAGCCTTAGGCTTATTGAGAAGAGCACGGATTTCCCGAGGCAAGAAAGAGGCTGATAGAGAGAGTTTTCCCTATAGGCTGCTTTCCTCTTGGCATATAGCCCCAATCCACCTGCCGTTGCTGGGACAGAACTGCTGGCCACACCTGTCAGAAGGATTTAGTGTTTCTCTGTGGTTTAATGTGGAATACATCCATGAATCCGAGAGTGCTGCAGAAAGGGGAAAAAGAGTAAAGAAAAGAAACAAACCATCAGTTCTGGAAGACAGCAGTTTTGAAGGAGCAGAAGGTGATAGACCAGAAGTTACAGAATCCATCAATCCTGGTGACAGACTCATAGAAGACGGCTGTATTCATTTGATTTCACTGGGGTCCAAAGCATTGATGATCCAAGTGTGGGCTGATCCCCACAGTGGCACTTTTATCTTTCGTGTGTGCATGGACTCAAATGATGACACGAAAGCTGTCTCACTAGCACAGGTGGAATCACAGGAGAATATTTTCTTTCCAAGCAAATGGCAACACTTAGTACTTACCTATATTCAGCATCCTCAAGGGAAAAAGAATGTCCATGGGGAAATCTCCATATGGGTCTCTGGGCAGAGGGTAATAAGAGATTTGTTTTTAAGTTGTAATGGAGTAAACCAAATAATTGAACTAAATTACTTAGATGGGATTCGAAGTCATTCCCTGAAAGCATTTGAAACTCTGATTGTCAGCCTAGGGGAACAACAGAAAGATGCTGCAGTTCTAGACGTCGATGGGTTAGACATCCAACAGGAGTTGCCGTCCTTAAGTGTGGGTCCTTCTCTTCATAAGCAGCAAGCTTCTTCAGATTCTCCTTGCAGTCTCAGGAAGTTTTATGCCAGCCTCAGAGAGCCTGATCCAAAAAAACGAAAGACCATTCACCAGGATGTTCACATAAACACCATAAACCTCTTCCTCTGTGTGGCTTTTCTATGTGTCAGTAAAGAAGCAGACTCTGATAGGGAGTCTGCCAATGAGTCAGAAGATACTTCTGGCTATGACAGCACTGCCAGTGAGCCATTAAGTCACATGCTACCATGTCTGTCTCTTGAGAACGTTGTCTTACCTTCCCCTGAATGTTTGCACCATGCAGCAGACATTTGGTCCATGTGTCGTTGGATCTACATGTTGAACTCAGTCTTCCAGAAACAATTTCACAGGCTTGGTGGTTTCCAAGTGTGCCATGAATTAATATTTATGATAATCCAGAAACTATTCAGAAGTCATACAGAGGATCAAGGAAGAAGGCAGGGAGAAATGAGTAGAAATGAAAACCAAGAGCTAATCAGGATATCTTACCCCGAGCTGACACTGAAGGGAGATGTATCATCTGCAACAGCACCAGACCTGGGATTTCTGAGAAAGAGTGCTGACAGCGTGCGTGGATTCCAGTCACAGCCTGTGCTTCCCACAAGTGCAGAGCAGATTGTGGCTACTGAATCTGTTCCTGGGGAACGAAAGGCATTTATGAGTCAACAAAGTGAGACTTCTCTCCAGAGCATACGACTTTTGGAGTCTCTCCTGGCCATTTGTCTTCATAGTGCCAGAGCCTGTCAACAGAAGATGGAATTGGAGCTACCGTCTCAGGGCTTGTCTGTGGAAAATATATTGTGTGAACTGAGGGAACACCTTTCCCAGTCAAAGGTGGCAGAAACAGAATTAGCAAAGCCTTTATTTGATGCCCTGCTTCGAGTAGCCCTGGGGAATCATTCAGCAGATTTGGGCCCTGGTGATGCTGTGACTGAGAAGAGTCATCCCTCTGAGGAAGAGCTGTTGTCCCAGCCCGGAGATTTTTCAGAAGAAGCTGAGGATTCTCAGTGTTGTAGTTTGAAACTTCTGGGTGAGGAAGAAGGCTATGAAGCGGATAGTGAAAGCAATCCTGAGGATGTTGACACCCAAGACGATGGAGTAGAATTAAATCCTGAAGCAGAAGGTTTCAGTGGATCGATTGTTTCAAACAACTTACTTGAAAACCTCACTCACGGGGAAATAATATACCCTGAGATTTGCATGCTGGGATTAAATTTGCTTTCTGCTAGCAAAGCTAAACTTGATGTGCTTGCTCATGTGTTTGAGAGCTTTCTGAAAATTGTCAGGCAGAAGGAAAAGAACATTTCTCTCCTCATACAACAGGGAACTGTGAAAATCCTTCTAGGCGGGTTCTTGAATATTTTAACACAAACTAACTCTGATTTCCAAGCATGCCAGAGAGTACTGGTGGATCTCTTGGTATCTTTGATGAGCTCAAGAACGTGTTCAGAAGACTTAACTCTTCTTTTGAGAATATTTCTGGAGAAATCTCCTTGTACAGAAATTCTTCTCCTTGGTATTCACAAAATTGTTGAAAGTGATTTTACTATGAGCCCTTCACAGTGTCTGACCTTTCCTTTCCTGCATACCCCGAGTTTAAGCAATGGTGTCTTATCACAGAAACCTCCTGGGATTCTTAACAGTAAAGCCTTAGGCTTATTGAGAAGAGCACGGATTTCCCGAGGCAAGAAAGAGGCTGATAGAGAGAGTTTTCCCTATAGGCTGCTTTCCTCTTGGCATATAGCCCCAATCCACCTGCCGTTGCTGGGACAGAACTGCTGGCCACACCTGTCAGAAGGATTTAGTGTTTCTCTGTGGTTTAATGTGGAATACATCCATGAATCCGAGAGTGCTGCAGAAAGGGGAAAAAGAGTAAAGAAAAGAAACAAACCATCAGTTCTGGAAGACAGCAGTTTTGAAGGAGCAGAAGGTGATAGACCAGAAGTTACAGAATCCATCAATCCTGGTGACAGACTCATAGAAGACGGCTGTATTCATTTGATTTCACTGGGGTCCAAAGCATTGATGATCCAAGTGTGGGCTGATCCCCACAGTGGCACTTTTATCTTTCGTGTGTGCATGGACTCAAATGATGACACGAAAGCTGTCTCACTAGCACAGGTGGAATCACAGGAGAATATTTTCTTTCCAAGCAAATGGCAACACTTAGTACTTACCTATATTCAGCATCCTCAAGGGAAAAAGAATGTCCATGGGGAAATCTCCATATGGGTCTCTGGGCAG
Peptide sequence:
MLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAEGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQRVIRDLFLSCNGVNQIIELNYLDGIRSHSLKAFETLIVSLGEQQKDAAVLDVDGLDIQQELPSLSVGPSLHKQQASSDSPCSLRKFYASLREPDPKKRKTIHQDVHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPCLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSRNENQELIRISYPELTLKGDVSSATAPDLGFLRKSADSVRGFQSQPVLPTSAEQIVATESVPGERKAFMSQQSETSLQSIRLLESLLAICLHSARACQQKMELELPSQGLSVENILCELREHLSQSKVAETELAKPLFDALLRVALGNHSADLGPGDAVTEKSHPSEEELLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDVDTQDDGVELNPEAEGFSGSIVSNNLLENLTHGEIIYPEICMLGLNLLSASKAKLDVLAHVFESFLKIVRQKEKNISLLIQQGTVKILLGGFLNILTQTNSDFQACQRVLVDLLVSLMSSRTCSEDLTLLLRIFLEKSPCTEILLLGIHKIVESDFTMSPSQCLTFPFLHTPSLSNGVLSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWHIAPIHLPLLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKRVKKRNKPSVLEDSSFEGAEGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCMDSNDDTKAVSLAQVESQENIFFPSKWQHLVLTYIQHPQGKKNVHGEISIWVSGQ
IRESfinder result
IRESfinder score
0.755563365
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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