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mmu_circLuzp1_3163
Basic information of circular RNA
PanCircBase ID:
mmu_circLuzp1_3163
mm39_Chr. Position ID:
chr4|136267666|136270829|+
Gene name:
Luzp1
Transcript:
ENSMUST00000170102.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
3163
Exon/intron sizes in nt:
3163
Flanking exon/introns:
chr4:136266527-136267666|chr4:136270829-136272548
Splice length:
3163
Other database IDs:
circBase: mmu_circ_0011308, CIRCpedia: MMU_CIRCpedia_8424, circAtlas: mmu-Luzp1_0003
Human Ortholog IDs:
Mature spliced Sequence:
ACTGATAATGGTCTTCAGTTGCCAAAGAAGGTTGTGGATGCCAAGAGAAAGGTAACCAGTTTCTCCAGTGCAGTTGGATGTCTCTAGCAGCCCATCGTGACTGGCTGTAGACATGGCAGAACTCACTAACTACAAGGATGCTGCCTCCAACCGCCACCTGCGATTTAAGTTACAGAGCCTGAGCCGCCGCCTCGATGAGTTAGAGGAAGCTACAAAAAACCTCCAGAGAGCGGAGGATGAGCTCCTGGACCTCCAGGACAAGGTGATCCAGGCAGAGGGCAGCGACTCCAGCACGCTGGCTGAGATCGAGGTGCTGCGCCAGCGGGTACTGAAGATTGAAGGTAAAGATGAGGAAATTAAGAGAGCAGAGGATCTATGTCATACGATGAAGGAGAAACTTGAAGAGGAAGAAAACCTTACGCGAGAGCTAAAATCTGAAATTGAGCGGCTTCAGAAACGAATGGTTGATTTAGAGAAGCTAGAGGAGGCTTTGAGCAGGAGTAAGAATGAATGTTCCCAGCTTTGTTTGAGCCTCAATGAAGAGAGAAATCTGACCAAGAAAATCTCTTCTGAGTTGGAAATGCTCAGAGTCAAAGTGAAAGAGCTAGAATCTTCAGAGGACCGCTTAGATAAAACTGAGCAGAGTTTAGTGTCAGAATTAGAAAAGCTAAAATCATTAACCCTGAGCTTTGTAAATGAGAGAAAATACTTGAATGAAAAGGAGAAAGAAAATGAGAAAATAATAAAAGAGCTTACTCAAAAACTGGAGCAGAACAAGAAAATGAACCGAGACCACATGAGAAATGCTTCCACTTTTCTGGAAAGGAATGACCTACGGATAGAGGACGGCATTTCCTCTACACTGTCATCCAAAGAGTCAAAAAGGAAGGGCAGTCTAGACTACCTAAAGCAGGTCGAGAATGAAACCAGAGACAAATCAGAAAATGAAAAGAACCGGAATCAGGAAGACAACAAAGTGAAAGACCTTAACCAAGAGATTGAGAAACTTAAGACACAAATCAAACATTTTGAATCTTTGGAAGAGGAGCTTAAGAAAATGAGGGCTAAAAACAATGACCTTCAGGATAATTACCTAACTGAGCTGAATAGAAACAGAAGCTTAGCCAGCCAGCTAGAGGAGATAAAGCTGCAGGTCAGGAAACAAAAAGAGTTAGGAAATGGAGACATAGAAGGTGAAGATGCTTTCCTTTTGGGCAGAGGCAGGCACGAGAGGACTAAGCTCAAAGGCCATGGGAGTGAGGCCTCTGTGTCCAAGCATACATCACGGGAACTGTCTCCCCAGCATAAGCGGGAAAGGCTCCGGAACAGGGAGTTTGCTCTCAGTAACGAACACTATTCTCTAAGCAGCAAACAGGCTTCTTCTCCAGTTTTCACAAACAAAAGGGCAGCAAAAGCCTCCAACATGGGAATGGGTACAGACAGTGGGACTCAGGAGACAAAGAGAACTGAAGACCGATTTGCACCTGGGTCCTCTCACAGTGAAGGGAAAAGGGGTAGGGAGCAGCCATCAGTGCTTAGCCGTTACCCCCCTGCTGCCCAGGAGCACACTAAAGTTTGGAAAGGAGCTCCCAAGCCAGGCACTGAGAGTGGACTTAAGGGAAAAGTAGAGAAGACAACACGAACATTTAGTGATTCCACTCATGTATCTGTGCCCAATGATATAGTGGGGAAAGGTGATAAGACTTCTGACCTCTCCTCTGAGGCACACTGTGGGAAGAGAGGACAGGTGCCTGGCCATGCAAGTCAGGGAACACAGGCTGTAGAGTCTAGCTGCTCTAAGGCCATCGGAGCTCTCTCCTCATCTCAAAAAGCCTCCTCAGAAGGACTTTCTAAGGGTAAAAAGACTGCCAATGGCCTAGCGGCTGATGCCAATTTTTCAAATTCTAAAGCTCCAATTTTATCAAAATATCCTTATAGTTCAAGAAGCCAAGAAAATATACTTCAGGGCTTTTCATTGCCAAATAAAGAAGGAGTTGATCAGCCTGTAGCAGTTGTGATGGAAGACAGCAGTCAGCATGAAGCCCTGAGGTGCCGTGTCATCAAATCCAGTGGCAGAGAGAAGCCAGACTCAGATGATGACCTGGACATAGAGTCTTTTGTTACTGCCAAGTTGGTGAACACAACTATCACTCCAGAGCCAGAGCCCAAACCACAGCCAAACTCAAGAGAAAAGGTTAAGTCCAGAGGGGGAACTAGAACTGCCCTATTTGAAAATGATAAAAATGCTGCAATAGAAAATGACTCTGTGAAACCTACCAGGCCTTCCTCCAATGCTATAGAATTCCCAGATGCCAACTGTGCTGGGGTGAAAAATCAAAGGCCCTTCAGCCCTAGAGAGGCCCTGCGGTCTAGAGCAATTATAAAACCCGTTATCATTGATAAGGATGTGAAAAAAATCATGGGTGGATCTGGAACTGAGGTTGTATTGGAGAAGCAGAAATCCACCTCTAAATCTGTGACAAGCAAAGTGACAAGCAGCATTACCATCTACCCCTCTGACAGCAGTGGGCCTAGAGCCGTCCCGAGCGAGGCCCCAAGGGAGAGGCATACATCCACCAGCAACATCCAGGTGGGGCCCCCAGAGCTCACAGCAATTAGCAACCATGTCAGCTCCCCTCTTGAGCTCTCTATTCATAAACATGACATCACCTTGCAGCTCACAGAAGCTGAAAGAGTGGGAGATGGGTCTCCAAAGAATAGAGCAGAGATGGTGGTCTCTAGGAGTAGCATTCTAATCAAGCCATCAGAGTCTGTGGAGAAGAATAGTCATGTGCCCCCAGCCGAGACAATCAGGTGGAAAAGCCATAGTGCCTCTTCAGACAGCAGACACATCACAGTGCGGAATGCCTGGAAGAGTAAGAGAGACTTGAAATGTTCAGAAGACCCCCCGACTGGAATAGGTAGGAACATGGAAGCTACTAACGCCTATACGCAGAGGCCCTGCACAGACTTCTTAGAACTTGAGCAGCCCAGGTCACAGCCATCTGAACAGGGCGCTCGAAGAGTAGGAAATTCAGGGGATGCTCCTGAGCTCTCCCCGAGGAGGACCCAAAGTAGCCTCACCGCATCAGAGGTGCTCACCCGCAGGGACCGGATGGGAGGTGCCATCACTGCTGCCTCCTGCAACCACTCATCAAGCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GATGGGAGGTGCCATCACTG
60.47
CACGATGGGCTGCTAGAGAC
60.25
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GATGGGAGGTGCCATCACTG
60.47
TGGGCTGCTAGAGACATCCA
60.33
143
PCR template sequence:
GAGGACCCAAAGTAGCCTCACCGCATCAGAGGTGCTCACCCGCAGGGACCGGATGGGAGGTGCCATCACTGCTGCCTCCTGCAACCACTCATCAAGCATGACTGATAATGGTCTTCAGTTGCCAAAGAAGGTTGTGGATGCCAAGAGAAAGGTAACCAGTTTCTCCAGTGCAGTTGGATGTCTCTAGCAGCCCATCGTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
26
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-296-5p(1), mmu-miR-135a-5p(1), mmu-miR-19b-3p(1), mmu-miR-19a-3p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-324-5p(1), mmu-miR-326-3p(1), mmu-miR-181b-5p(1), mmu-miR-433-3p(1), mmu-miR-29b-1-5p(1), mmu-miR-182-5p(1), mmu-miR-194-5p(1), mmu-miR-370-3p(1), mmu-miR-425-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-802-5p(1), mmu-miR-1198-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-1199-5p(1), mmu-miR-540-3p(1), mmu-miR-670-5p(1), mmu-miR-1904(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCATCAAGCATGACTGATAATGGTC
siRNA sets
GACCATTATCAGTCATGCT, CATTATCAGTCATGCTTGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCLUZP1_3163_ORF_1
0.9811
0.203316704
0.999999998
3138
ORF sequence:
ATGGCAGAACTCACTAACTACAAGGATGCTGCCTCCAACCGCCACCTGCGATTTAAGTTACAGAGCCTGAGCCGCCGCCTCGATGAGTTAGAGGAAGCTACAAAAAACCTCCAGAGAGCGGAGGATGAGCTCCTGGACCTCCAGGACAAGGTGATCCAGGCAGAGGGCAGCGACTCCAGCACGCTGGCTGAGATCGAGGTGCTGCGCCAGCGGGTACTGAAGATTGAAGGTAAAGATGAGGAAATTAAGAGAGCAGAGGATCTATGTCATACGATGAAGGAGAAACTTGAAGAGGAAGAAAACCTTACGCGAGAGCTAAAATCTGAAATTGAGCGGCTTCAGAAACGAATGGTTGATTTAGAGAAGCTAGAGGAGGCTTTGAGCAGGAGTAAGAATGAATGTTCCCAGCTTTGTTTGAGCCTCAATGAAGAGAGAAATCTGACCAAGAAAATCTCTTCTGAGTTGGAAATGCTCAGAGTCAAAGTGAAAGAGCTAGAATCTTCAGAGGACCGCTTAGATAAAACTGAGCAGAGTTTAGTGTCAGAATTAGAAAAGCTAAAATCATTAACCCTGAGCTTTGTAAATGAGAGAAAATACTTGAATGAAAAGGAGAAAGAAAATGAGAAAATAATAAAAGAGCTTACTCAAAAACTGGAGCAGAACAAGAAAATGAACCGAGACCACATGAGAAATGCTTCCACTTTTCTGGAAAGGAATGACCTACGGATAGAGGACGGCATTTCCTCTACACTGTCATCCAAAGAGTCAAAAAGGAAGGGCAGTCTAGACTACCTAAAGCAGGTCGAGAATGAAACCAGAGACAAATCAGAAAATGAAAAGAACCGGAATCAGGAAGACAACAAAGTGAAAGACCTTAACCAAGAGATTGAGAAACTTAAGACACAAATCAAACATTTTGAATCTTTGGAAGAGGAGCTTAAGAAAATGAGGGCTAAAAACAATGACCTTCAGGATAATTACCTAACTGAGCTGAATAGAAACAGAAGCTTAGCCAGCCAGCTAGAGGAGATAAAGCTGCAGGTCAGGAAACAAAAAGAGTTAGGAAATGGAGACATAGAAGGTGAAGATGCTTTCCTTTTGGGCAGAGGCAGGCACGAGAGGACTAAGCTCAAAGGCCATGGGAGTGAGGCCTCTGTGTCCAAGCATACATCACGGGAACTGTCTCCCCAGCATAAGCGGGAAAGGCTCCGGAACAGGGAGTTTGCTCTCAGTAACGAACACTATTCTCTAAGCAGCAAACAGGCTTCTTCTCCAGTTTTCACAAACAAAAGGGCAGCAAAAGCCTCCAACATGGGAATGGGTACAGACAGTGGGACTCAGGAGACAAAGAGAACTGAAGACCGATTTGCACCTGGGTCCTCTCACAGTGAAGGGAAAAGGGGTAGGGAGCAGCCATCAGTGCTTAGCCGTTACCCCCCTGCTGCCCAGGAGCACACTAAAGTTTGGAAAGGAGCTCCCAAGCCAGGCACTGAGAGTGGACTTAAGGGAAAAGTAGAGAAGACAACACGAACATTTAGTGATTCCACTCATGTATCTGTGCCCAATGATATAGTGGGGAAAGGTGATAAGACTTCTGACCTCTCCTCTGAGGCACACTGTGGGAAGAGAGGACAGGTGCCTGGCCATGCAAGTCAGGGAACACAGGCTGTAGAGTCTAGCTGCTCTAAGGCCATCGGAGCTCTCTCCTCATCTCAAAAAGCCTCCTCAGAAGGACTTTCTAAGGGTAAAAAGACTGCCAATGGCCTAGCGGCTGATGCCAATTTTTCAAATTCTAAAGCTCCAATTTTATCAAAATATCCTTATAGTTCAAGAAGCCAAGAAAATATACTTCAGGGCTTTTCATTGCCAAATAAAGAAGGAGTTGATCAGCCTGTAGCAGTTGTGATGGAAGACAGCAGTCAGCATGAAGCCCTGAGGTGCCGTGTCATCAAATCCAGTGGCAGAGAGAAGCCAGACTCAGATGATGACCTGGACATAGAGTCTTTTGTTACTGCCAAGTTGGTGAACACAACTATCACTCCAGAGCCAGAGCCCAAACCACAGCCAAACTCAAGAGAAAAGGTTAAGTCCAGAGGGGGAACTAGAACTGCCCTATTTGAAAATGATAAAAATGCTGCAATAGAAAATGACTCTGTGAAACCTACCAGGCCTTCCTCCAATGCTATAGAATTCCCAGATGCCAACTGTGCTGGGGTGAAAAATCAAAGGCCCTTCAGCCCTAGAGAGGCCCTGCGGTCTAGAGCAATTATAAAACCCGTTATCATTGATAAGGATGTGAAAAAAATCATGGGTGGATCTGGAACTGAGGTTGTATTGGAGAAGCAGAAATCCACCTCTAAATCTGTGACAAGCAAAGTGACAAGCAGCATTACCATCTACCCCTCTGACAGCAGTGGGCCTAGAGCCGTCCCGAGCGAGGCCCCAAGGGAGAGGCATACATCCACCAGCAACATCCAGGTGGGGCCCCCAGAGCTCACAGCAATTAGCAACCATGTCAGCTCCCCTCTTGAGCTCTCTATTCATAAACATGACATCACCTTGCAGCTCACAGAAGCTGAAAGAGTGGGAGATGGGTCTCCAAAGAATAGAGCAGAGATGGTGGTCTCTAGGAGTAGCATTCTAATCAAGCCATCAGAGTCTGTGGAGAAGAATAGTCATGTGCCCCCAGCCGAGACAATCAGGTGGAAAAGCCATAGTGCCTCTTCAGACAGCAGACACATCACAGTGCGGAATGCCTGGAAGAGTAAGAGAGACTTGAAATGTTCAGAAGACCCCCCGACTGGAATAGGTAGGAACATGGAAGCTACTAACGCCTATACGCAGAGGCCCTGCACAGACTTCTTAGAACTTGAGCAGCCCAGGTCACAGCCATCTGAACAGGGCGCTCGAAGAGTAGGAAATTCAGGGGATGCTCCTGAGCTCTCCCCGAGGAGGACCCAAAGTAGCCTCACCGCATCAGAGGTGCTCACCCGCAGGGACCGGATGGGAGGTGCCATCACTGCTGCCTCCTGCAACCACTCATCAAGCATGACTGATAATGGTCTTCAGTTGCCAAAGAAGGTTGTGGATGCCAAGAGAAAGGTAACCAGTTTCTCCAGTGCAGTTGGATGTCTCTAG
Peptide sequence:
MAELTNYKDAASNRHLRFKLQSLSRRLDELEEATKNLQRAEDELLDLQDKVIQAEGSDSSTLAEIEVLRQRVLKIEGKDEEIKRAEDLCHTMKEKLEEEENLTRELKSEIERLQKRMVDLEKLEEALSRSKNECSQLCLSLNEERNLTKKISSELEMLRVKVKELESSEDRLDKTEQSLVSELEKLKSLTLSFVNERKYLNEKEKENEKIIKELTQKLEQNKKMNRDHMRNASTFLERNDLRIEDGISSTLSSKESKRKGSLDYLKQVENETRDKSENEKNRNQEDNKVKDLNQEIEKLKTQIKHFESLEEELKKMRAKNNDLQDNYLTELNRNRSLASQLEEIKLQVRKQKELGNGDIEGEDAFLLGRGRHERTKLKGHGSEASVSKHTSRELSPQHKRERLRNREFALSNEHYSLSSKQASSPVFTNKRAAKASNMGMGTDSGTQETKRTEDRFAPGSSHSEGKRGREQPSVLSRYPPAAQEHTKVWKGAPKPGTESGLKGKVEKTTRTFSDSTHVSVPNDIVGKGDKTSDLSSEAHCGKRGQVPGHASQGTQAVESSCSKAIGALSSSQKASSEGLSKGKKTANGLAADANFSNSKAPILSKYPYSSRSQENILQGFSLPNKEGVDQPVAVVMEDSSQHEALRCRVIKSSGREKPDSDDDLDIESFVTAKLVNTTITPEPEPKPQPNSREKVKSRGGTRTALFENDKNAAIENDSVKPTRPSSNAIEFPDANCAGVKNQRPFSPREALRSRAIIKPVIIDKDVKKIMGGSGTEVVLEKQKSTSKSVTSKVTSSITIYPSDSSGPRAVPSEAPRERHTSTSNIQVGPPELTAISNHVSSPLELSIHKHDITLQLTEAERVGDGSPKNRAEMVVSRSSILIKPSESVEKNSHVPPAETIRWKSHSASSDSRHITVRNAWKSKRDLKCSEDPPTGIGRNMEATNAYTQRPCTDFLELEQPRSQPSEQGARRVGNSGDAPELSPRRTQSSLTASEVLTRRDRMGGAITAASCNHSSSMTDNGLQLPKKVVDAKRKVTSFSSAVGCL
IRESfinder result
IRESfinder score
0.471883945
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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