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mmu_circLuc7l2_623
Basic information of circular RNA
PanCircBase ID:
mmu_circLuc7l2_623
mm39_Chr. Position ID:
chr6|38561975|38570404|+
Gene name:
Luc7l2
Transcript:
ENSMUST00000163047.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
4,5,6,7,8
Exon/intron offsets:
0,4135,6247,7578,8337
Genomic length:
8429
Exon/intron sizes in nt:
99,111,144,177,92
Flanking exon/introns:
chr6:38547539-38561975|chr6:38570404-38580211
Splice length:
623
Other database IDs:
CIRCpedia: MMU_CIRCpedia_18939, circAtlas: mmu-Luc7l2_0006
circBase: hsa_circ_0133534, circAtlas: hsa-C7orf55-LUC7L2_0001, CIRCpedia: HSA_CIRCpedia_56976
Human Ortholog IDs:
Mature spliced Sequence:
AGAATGGATCTTGGAGAATGCTTGAAAGTCCATGACCTGGCTTTAAGAGCAGATTATGAAATTGCGTCCAAAGAACAAGACTTTTTCTTTGAACTTGATGCCATGGATCATCTGCAGTCATTCATTGCAGATTGTGATAGAAGAACAGAAGTATCTAAGAAAAGACTAGCAGAAACTCAAGAAGAGATAAGTGCTGAAGTGGCTGCAAAGGCAGAGCGTGTTCATGAGTTAAATGAGGAAATAGGCAAGTTGTTGGCCAAAGTGGAACAACTAGGAGCTGAAGGAAATGTGGAAGAATCTCAGAAAGTCATGGATGAAGTCGAGAAAGCACGGGCAAAGAAAAGAGAAGCAGAGGAAGTTTATCGGAACTCTATGCCAGCTTCCAGCTTCCAGCAGCAGAAACTTCGTGTCTGCGAGGTCTGCTCTGCGTATTTAGGTCTTCATGACAATGACAGGCGACTGGCTGATCATTTTGGGGGTAAATTGCACCTGGGATTTATTGAAATAAGAGAGAAGCTGGAAGAATTAAAGAGAGTTGTAGCTGAGAAGCAGGAGAAGAGAAACCAGGAACGTCTGAAACGAAGAGAAGAGAGGGAGAGAGAAGAACGGGAGAAACTGAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGGAGAAGAGAAACCAGGA
59.37
AGTCTTGTTCTTTGGACGCA
57.67
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGGAGAAGAGAAACCAGG
59.37
AGTCTTGTTCTTTGGACGCA
57.67
158
PCR template sequence:
AATTAAAGAGAGTTGTAGCTGAGAAGCAGGAGAAGAGAAACCAGGAACGTCTGAAACGAAGAGAAGAGAGGGAGAGAGAAGAACGGGAGAAACTGAGGAGAGAATGGATCTTGGAGAATGCTTGAAAGTCCATGACCTGGCTTTAAGAGCAGATTATGAAATTGCGTCCAAAGAACAAGACTTTTTCTTTGAACTTGATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-370-3p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
AGAAACTGAGGAGAGAATGGATCTTG
siRNA sets
CAAGATCCATTCTCTCCTC, AAGATCCATTCTCTCCTCA, AGATCCATTCTCTCCTCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCLUC7L2_623_ORF_1
1.0602
0.213829833
0.902748959
645
ORF sequence:
ATGGATCTTGGAGAATGCTTGAAAGTCCATGACCTGGCTTTAAGAGCAGATTATGAAATTGCGTCCAAAGAACAAGACTTTTTCTTTGAACTTGATGCCATGGATCATCTGCAGTCATTCATTGCAGATTGTGATAGAAGAACAGAAGTATCTAAGAAAAGACTAGCAGAAACTCAAGAAGAGATAAGTGCTGAAGTGGCTGCAAAGGCAGAGCGTGTTCATGAGTTAAATGAGGAAATAGGCAAGTTGTTGGCCAAAGTGGAACAACTAGGAGCTGAAGGAAATGTGGAAGAATCTCAGAAAGTCATGGATGAAGTCGAGAAAGCACGGGCAAAGAAAAGAGAAGCAGAGGAAGTTTATCGGAACTCTATGCCAGCTTCCAGCTTCCAGCAGCAGAAACTTCGTGTCTGCGAGGTCTGCTCTGCGTATTTAGGTCTTCATGACAATGACAGGCGACTGGCTGATCATTTTGGGGGTAAATTGCACCTGGGATTTATTGAAATAAGAGAGAAGCTGGAAGAATTAAAGAGAGTTGTAGCTGAGAAGCAGGAGAAGAGAAACCAGGAACGTCTGAAACGAAGAGAAGAGAGGGAGAGAGAAGAACGGGAGAAACTGAGGAGAGAATGGATCTTGGAGAATGCTTGA
Peptide sequence:
MDLGECLKVHDLALRADYEIASKEQDFFFELDAMDHLQSFIADCDRRTEVSKKRLAETQEEISAEVAAKAERVHELNEEIGKLLAKVEQLGAEGNVEESQKVMDEVEKARAKKREAEEVYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIEIREKLEELKRVVAEKQEKRNQERLKRREEREREEREKLRREWILENA
IRESfinder result
IRESfinder score
0.447902335
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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