top of page
mmu_circLgr4_501
Basic information of circular RNA
PanCircBase ID:
mmu_circLgr4_501
mm39_Chr. Position ID:
chr2|109821448|109830712|+
Gene name:
Lgr4
Transcript:
ENSMUST00000046548.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
4,5,6,7,8
Exon/intron offsets:
0,5450,6489,8302,9192
Genomic length:
9264
Exon/intron sizes in nt:
72,216,72,69,72
Flanking exon/introns:
chr2:109820121-109821448|chr2:109830712-109830904
Splice length:
501
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AACCCTCCAGAACAATCAGTTGAAAACAGTACCCAGTGAAGCCATTCGTGGACTGAGTGCTTTGCAGTCTCTACGCTTAGATGCCAACCATATTACCTCAGTCCCGGAGGACAGTTTTGAAGGGCTCGTTCAGTTGCGGCATCTGTGGCTGGATGACAACATCTTGACGGAAGTGCCTGTGCGTCCGCTCAGCAACCTGCCAACCCTGCAGGCGCTGACCTTGGCTCTCAACAACATCTCAAGCATCCCCGACTTCGCATTCACCAACCTTTCAAGCTTGGTAGTGCTGCATCTTCATAACAATAAAATTAAAAGCCTCAGTCAACACTGTTTTGATGGACTAGATAACCTGGAAACCCTGGACTTGAATTATAATAACTTGGATGAATTTCCTCAGGCTATTAAAGCCCTTCCCAGCCTTAAAGAGCTGGGATTTCACAGTAATTCTATTTCTGTTATCCCGGATGGAGCATTTGCTGGTAATCCACTGCTAAGAACTAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCTTCCCAGCCTTAAAGA
59.67
CACTCAGTCCACGAATGGCT
60.04
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCCTTCCCAGCCTTAAAG
59.67
CACTCAGTCCACGAATGGCT
60.04
155
PCR template sequence:
TTAAAGCCCTTCCCAGCCTTAAAGAGCTGGGATTTCACAGTAATTCTATTTCTGTTATCCCGGATGGAGCATTTGCTGGTAATCCACTGCTAAGAACTATAACCCTCCAGAACAATCAGTTGAAAACAGTACCCAGTGAAGCCATTCGTGGACTGAGTGCTTTGCAGTCTCTACGCTTAGATGCCAACCATATTACCTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-let-7d-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTAAGAACTATAACCCTCCAGAAC
siRNA sets
TTCTGGAGGGTTATAGTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.702079941
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page