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mmu_circLars_2183
Basic information of circular RNA
PanCircBase ID:
mmu_circLars_2183
mm39_Chr. Position ID:
chr18|42354543|42383974|-
Gene name:
Lars
Transcript:
ENSMUST00000097590.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
20
Exon/intron indexes:
23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,4275,5757,6527,7179,8150,8312,8463,12321,13102,14006,15104,15260,20424,21225,21488,22298,23270,24461,29350
Genomic length:
29431
Exon/intron sizes in nt:
184,64,58,213,139,83,66,86,78,141,54,77,88,226,68,64,113,162,138,81
Flanking exon/introns:
chr18:42353013-42354543|chr18:42383974-42384357
Splice length:
2183
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTTGCAGTAGGATACCAGAGATTAAAAGGAAAAAGCTGTCTGTTTCCCTTTGGGTTGCACTGTACTGGTATGCCTATTAAGGCATGCGCTGACAAGCTGAAAAGAGAAATAGAGCTGTATGGCTGCCCTCCTGATTTTCCCGAGGAAGAAGAGGAAGAGGAAGAAAGCAGTGCTAAACCTGGAGATATAGTAGTGAGGGATAAAGCCAAAGGGAAAAAGAGCAAAGCTGCCGCCAAAGCAGGATCTTCTAAGTACCAGTGGGACATTATGAAATCCCTTGGGCTCTCTGATGACGACATAGTGAAGTTTTCTGAAGCAGAGCACTGGCTTGATTATTTCCCACCACTGGCCGTTCAGGATCTGAAGACTATAGGTTTGAAGGTAGACTGGCGGCGCTCCTTCATCACCACGGACGTGAATCCTTACTACGATTCCTTTGTCAGGTGGCAGTTTTTAACTTTAAGAGAAAGAAATAAAATTAAATTTGGGAAGCGGTACACTATTTATTCCCCAAAAGATGGACAGCCTTGCATGGATCATGACAGACAAACTGGAGAGGGTGTTGGACCTCAGGAATATACTCTGGTCAAACTGAAGGTGCTTGAACCATATCCATCAAAATTAAGCGGCCTGAAAGGGAAAAATATCTTCCTAGTAGCTGCTACACTCAGACCTGAGACTATGTTTGGGCAGACAAATTGTTGGGTTCGGCCTGATATGAAATACATTGGGTTTGAGACAGCAAATGGAGACATCTTCATCTGTACCCAGAGAGCAGCCAGGAATATGTCATACCAAGGCTTCACCAAGCACAACGGAGTGGTGCCGGTTGTGAAGGAGTTAATGGGAGAGGAAATCCTTGGGGCTTCACTTTCTGCACCCCTGACGTGCTATAAGGTGGTCTACGTTCTCCCTATGCTGACCATTAAAGAGGATAAAGGCACTGGGGTGGTCACGAGCGTTCCTTCTGATTCCCCGGATGATCTTGCAGCACTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAACAAAGTTTGGAATTAGGGATGACATGGTCCTGCCGTTTGAACCGGTGCCAGTCCTGGAAATCCCAGGGATTGGAAATCTTCCTGCTGTGACTGTTTGTGATGAGCTGAAAATTCAGAGCCAGAATGACCGTGAAAAACTTGCAGAAGCAAAGGAGAAATTGTATTTAAGAGGATTTTATGATGGTGTAATGCTAGTGGATGGCTTTAAAGGACAGAAGATCCAACATGTAAAGAAGACTATTCAGAAAAACATGATTGATGCTGGAGATGCGCTTATCTACATGGAGCCAGAAAAACAAGTGATGTCGAGGTCTGCAGATGAGTGCGTCGTGGCCCTGTGTGACCAGTGGTACTTGGATTATGGAGATGAGAACTGGAAGAAACAGACATTTCAGTGCCTGAAGAACATGGAAACATTCTGCGAGGAGAGCAGGAAGAATTTTGAAGCTTCCTTAGACTGGCTACAAGAACATGCTTGCTCAAGAACGTACGGTTTAGGCACTCGTCTGCCTTGGGATGAACAGTGGTTAATCGAATCTCTCTCGGATTCCACAATTTACATGGCGTTCTACACAGTGGCACACCTGCTGCAGGGGGGTGACCTGAATGGGCAGGCGGAGTCTCCACTGGGCATCAGACCCCAGCAGATGACAAAGGACGTTTGGGATTATGTGTTCTTCAAGGATGCTCCATTTCCTAAGACTCAGATCCCAAAGGAGAAGCTGGATCAGTTGAAGCAGGAGTTTGAGTTTTGGTACCCAGTGGACCTTCGAGCCTCTGGCAAGGATCTGATTCCAAATCATCTTTCATATTACATTTACAACCATGTGGCCATGTGGCCAGAGCAAAGTGACAAGTGGCCCGTGTCTGTCCGAGCAAATGGACACCTGCTGCTCAACTCGGAGAAGATGTCAAAATCTACGGGCAACTTCCTCACTTTGAGCCAAGCTGTTGACAAATTTTCAGCTGACGGAATGCGCCTGGCTCTGGCTGATGCTGGTGACACTGTTGAAGATGCTAACTTTGTGGAAGCCATGGCAGACGCAGGCATCCTCCGTCTGTACACATGGGTAGAGTGGGTCAAAGAAATGCTCGCCAGCTGCAGCAGCCTCAGAAGTGGACCTGCCGACTCATTCAATGATCGTGTGTTTGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGTCAAAGAAATGCTCGCC
59.83
ACCAGTACAGTGCAACCCAA
59.45
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCCTCAGAAGTGGACCTG
60.04
CTTGTCAGCGCATGCCTTAA
59.2
147
PCR template sequence:
GTCTGTACACATGGGTAGAGTGGGTCAAAGAAATGCTCGCCAGCTGCAGCAGCCTCAGAAGTGGACCTGCCGACTCATTCAATGATCGTGTGTTTGCCAGTTTGCAGTAGGATACCAGAGATTAAAAGGAAAAAGCTGTCTGTTTCCCTTTGGGTTGCACTGTACTGGTATGCCTATTAAGGCATGCGCTGACAAGCTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
24
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-338-3p(2), mmu-miR-9-5p(1), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-375-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-130a-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-132-3p(1), mmu-miR-203-3p(1), mmu-miR-136-5p(1), mmu-miR-22-3p(1), mmu-miR-130b-3p(1), mmu-miR-721(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-362-5p(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-300-3p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTGTGTTTGCCAGTTTGCAGTAGGAT
siRNA sets
ATCCTACTGCAAACTGGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCLARS_2183_ORF_1
0.9307
0.232757055
0.999996839
2124
ORF sequence:
ATGCCTATTAAGGCATGCGCTGACAAGCTGAAAAGAGAAATAGAGCTGTATGGCTGCCCTCCTGATTTTCCCGAGGAAGAAGAGGAAGAGGAAGAAAGCAGTGCTAAACCTGGAGATATAGTAGTGAGGGATAAAGCCAAAGGGAAAAAGAGCAAAGCTGCCGCCAAAGCAGGATCTTCTAAGTACCAGTGGGACATTATGAAATCCCTTGGGCTCTCTGATGACGACATAGTGAAGTTTTCTGAAGCAGAGCACTGGCTTGATTATTTCCCACCACTGGCCGTTCAGGATCTGAAGACTATAGGTTTGAAGGTAGACTGGCGGCGCTCCTTCATCACCACGGACGTGAATCCTTACTACGATTCCTTTGTCAGGTGGCAGTTTTTAACTTTAAGAGAAAGAAATAAAATTAAATTTGGGAAGCGGTACACTATTTATTCCCCAAAAGATGGACAGCCTTGCATGGATCATGACAGACAAACTGGAGAGGGTGTTGGACCTCAGGAATATACTCTGGTCAAACTGAAGGTGCTTGAACCATATCCATCAAAATTAAGCGGCCTGAAAGGGAAAAATATCTTCCTAGTAGCTGCTACACTCAGACCTGAGACTATGTTTGGGCAGACAAATTGTTGGGTTCGGCCTGATATGAAATACATTGGGTTTGAGACAGCAAATGGAGACATCTTCATCTGTACCCAGAGAGCAGCCAGGAATATGTCATACCAAGGCTTCACCAAGCACAACGGAGTGGTGCCGGTTGTGAAGGAGTTAATGGGAGAGGAAATCCTTGGGGCTTCACTTTCTGCACCCCTGACGTGCTATAAGGTGGTCTACGTTCTCCCTATGCTGACCATTAAAGAGGATAAAGGCACTGGGGTGGTCACGAGCGTTCCTTCTGATTCCCCGGATGATCTTGCAGCACTCAGAGACTTGAAGAAAAAGCAAGCCTTACGAACAAAGTTTGGAATTAGGGATGACATGGTCCTGCCGTTTGAACCGGTGCCAGTCCTGGAAATCCCAGGGATTGGAAATCTTCCTGCTGTGACTGTTTGTGATGAGCTGAAAATTCAGAGCCAGAATGACCGTGAAAAACTTGCAGAAGCAAAGGAGAAATTGTATTTAAGAGGATTTTATGATGGTGTAATGCTAGTGGATGGCTTTAAAGGACAGAAGATCCAACATGTAAAGAAGACTATTCAGAAAAACATGATTGATGCTGGAGATGCGCTTATCTACATGGAGCCAGAAAAACAAGTGATGTCGAGGTCTGCAGATGAGTGCGTCGTGGCCCTGTGTGACCAGTGGTACTTGGATTATGGAGATGAGAACTGGAAGAAACAGACATTTCAGTGCCTGAAGAACATGGAAACATTCTGCGAGGAGAGCAGGAAGAATTTTGAAGCTTCCTTAGACTGGCTACAAGAACATGCTTGCTCAAGAACGTACGGTTTAGGCACTCGTCTGCCTTGGGATGAACAGTGGTTAATCGAATCTCTCTCGGATTCCACAATTTACATGGCGTTCTACACAGTGGCACACCTGCTGCAGGGGGGTGACCTGAATGGGCAGGCGGAGTCTCCACTGGGCATCAGACCCCAGCAGATGACAAAGGACGTTTGGGATTATGTGTTCTTCAAGGATGCTCCATTTCCTAAGACTCAGATCCCAAAGGAGAAGCTGGATCAGTTGAAGCAGGAGTTTGAGTTTTGGTACCCAGTGGACCTTCGAGCCTCTGGCAAGGATCTGATTCCAAATCATCTTTCATATTACATTTACAACCATGTGGCCATGTGGCCAGAGCAAAGTGACAAGTGGCCCGTGTCTGTCCGAGCAAATGGACACCTGCTGCTCAACTCGGAGAAGATGTCAAAATCTACGGGCAACTTCCTCACTTTGAGCCAAGCTGTTGACAAATTTTCAGCTGACGGAATGCGCCTGGCTCTGGCTGATGCTGGTGACACTGTTGAAGATGCTAACTTTGTGGAAGCCATGGCAGACGCAGGCATCCTCCGTCTGTACACATGGGTAGAGTGGGTCAAAGAAATGCTCGCCAGCTGCAGCAGCCTCAGAAGTGGACCTGCCGACTCATTCAATGATCGTGTGTTTGCCAGTTTGCAGTAG
Peptide sequence:
MPIKACADKLKREIELYGCPPDFPEEEEEEEESSAKPGDIVVRDKAKGKKSKAAAKAGSSKYQWDIMKSLGLSDDDIVKFSEAEHWLDYFPPLAVQDLKTIGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLVKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETANGDIFICTQRAARNMSYQGFTKHNGVVPVVKELMGEEILGASLSAPLTCYKVVYVLPMLTIKEDKGTGVVTSVPSDSPDDLAALRDLKKKQALRTKFGIRDDMVLPFEPVPVLEIPGIGNLPAVTVCDELKIQSQNDREKLAEAKEKLYLRGFYDGVMLVDGFKGQKIQHVKKTIQKNMIDAGDALIYMEPEKQVMSRSADECVVALCDQWYLDYGDENWKKQTFQCLKNMETFCEESRKNFEASLDWLQEHACSRTYGLGTRLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGDLNGQAESPLGIRPQQMTKDVWDYVFFKDAPFPKTQIPKEKLDQLKQEFEFWYPVDLRASGKDLIPNHLSYYIYNHVAMWPEQSDKWPVSVRANGHLLLNSEKMSKSTGNFLTLSQAVDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMLASCSSLRSGPADSFNDRVFASLQ
IRESfinder result
IRESfinder score
0.503223084
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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