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mmu_circLarge1_281
Basic information of circular RNA
PanCircBase ID:
mmu_circLarge1_281
mm39_Chr. Position ID:
chr8|73940752|74066914|-
Gene name:
Large1
Transcript:
ENSMUST00000212459.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,126069
Genomic length:
126162
Exon/intron sizes in nt:
188,93
Flanking exon/introns:
chr8:73858760-73940752|chr8:74066914-74079093
Splice length:
281
Other database IDs:
CIRCpedia: MMU_CIRCpedia_28328, circAtlas: mmu-Large_0024
Human Ortholog IDs:
Mature spliced Sequence:
GTACCCACAATGGGCATTGGATCCCCTGGAACTGGCAGTAGTGAGCCACCTAACGTGGGTACAAGGAGTTGATCCCAAGTCCTCTTGAGAGCAAAAGTATTATGAGGGATGCTGAGGAATTCTTGCTCTGCACAGAGACACCACATTGAAATTAGAGATTGGCACTTCTGAGAGGATGCTGGGAATCTGCAGAGGGAGAAGGAAATTCCTGGCTGCCTCTTTGACTCTACTCTGCATACCAGCCATCACCTGGATTTACTTGTTTGCTGGGAGCTTTGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAATCTGCAGAGGGAGAAGG
59.86
CACTACTGCCAGTTCCAGGG
60.04
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAATCTGCAGAGGGAGAAGG
59.86
TACTGCCAGTTCCAGGGGAT
60.25
140
PCR template sequence:
GGAATCTGCAGAGGGAGAAGGAAATTCCTGGCTGCCTCTTTGACTCTACTCTGCATACCAGCCATCACCTGGATTTACTTGTTTGCTGGGAGCTTTGAAGGTACCCACAATGGGCATTGGATCCCCTGGAACTGGCAGTAGTGAGCCACCTAACGTGGGTACAAGGAGTTGATCCCAAGTCCTCTTGAGAGCAAAAGTAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-709(1), mmu-miR-324-5p(1), mmu-miR-369-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGAGCTTTGAAGGTACCCACAATGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCLARGE1_281_ORF_1
0.9436
-0.148584081
0.045296107
147
ORF sequence:
ATGCTGGGAATCTGCAGAGGGAGAAGGAAATTCCTGGCTGCCTCTTTGACTCTACTCTGCATACCAGCCATCACCTGGATTTACTTGTTTGCTGGGAGCTTTGAAGGTACCCACAATGGGCATTGGATCCCCTGGAACTGGCAGTAG
Peptide sequence:
MLGICRGRRKFLAASLTLLCIPAITWIYLFAGSFEGTHNGHWIPWNWQ
IRESfinder result
IRESfinder score
0.74315563
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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