top of page
mmu_circKmt2e_639
Basic information of circular RNA
PanCircBase ID:
mmu_circKmt2e_639
mm39_Chr. Position ID:
chr5|23687399|23697430|+
Gene name:
Kmt2e
Transcript:
ENSMUST00000094962.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
12,13,14,15
Exon/intron offsets:
0,2945,3900,9866
Genomic length:
10031
Exon/intron sizes in nt:
11,02,65,99,165
Flanking exon/introns:
chr5:23686324-23687399|chr5:23697430-23698196
Splice length:
639
Other database IDs:
circBase: mmu_circ_0012442, CIRCpedia: MMU_CIRCpedia_13900, circAtlas: mmu-Kmt2e_0044
Human Ortholog IDs:
Mature spliced Sequence:
GTGAGGCATGAAATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCATTGCCTTTGATTTTGACTACGGGAATTGCAAATACAAGGTGGACTGTGCATGCCTCAAGGAAAATCCAGAGTGCCCTGTTCTCAAGCGAAGCTCGGAATCCACAGAAAACATCAATAGTGGTTATGAGACCAGAAGGAAAAAAGGAAAAAAAGAGAAAGATACTTCAAAAGAAAAAGACATACAAAATCAGAATATGACTTTGGACTGTGAAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGTGTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAAAGGATCAGCACAGCT
59.31
AGGCAATGGTAATTTCAGTCCCT
59.99
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAAAGGATCAGCACAGCT
59.31
GGCAATGGTAATTTCAGTCCCT
58.63
170
PCR template sequence:
AAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGTGAGGCATGAAATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCATTGCCTTTGATTTTGACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-129-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
CGAAGTGGTGCAGGTGAGGCATGAAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKMT2E_639_ORF_1
1.0441
0.195637114
0.990980522
1002
ORF sequence:
ATGACTTTGGACTGTGAAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGTGTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGTGAGGCATGAAATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCATTGCCTTTGATTTTGACTACGGGAATTGCAAATACAAGGTGGACTGTGCATGCCTCAAGGAAAATCCAGAGTGCCCTGTTCTCAAGCGAAGCTCGGAATCCACAGAAAACATCAATAGTGGTTATGAGACCAGAAGGAAAAAAGGAAAAAAAGAGAAAGATACTTCAAAAGAAAAAGACATACAAAATCAGAATATGACTTTGGACTGTGAAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGTGTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAG
Peptide sequence:
MTLDCEGTNNKIRSPETKQRKLSPLRLSVSNNQEPDFIDDMEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQVRHEIEEGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESTENINSGYETRRKKGKKEKDTSKEKDIQNQNMTLDCEGTNNKIRSPETKQRKLSPLRLSVSNNQEPDFIDDMEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQ
IRESfinder result
IRESfinder score
0.439046341
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page