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mmu_circKmt2e_352
Basic information of circular RNA
PanCircBase ID:
mmu_circKmt2e_352
mm39_Chr. Position ID:
chr5|23672105|23681522|+
Gene name:
Kmt2e
Transcript:
ENSMUST00000196889.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,6,7,8
Exon/intron offsets:
0,4640,6373,9378
Genomic length:
9417
Exon/intron sizes in nt:
81,59,173,39
Flanking exon/introns:
chr5:23669930-23672105|chr5:23681522-23683511
Splice length:
352
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGTTTGGCAACATATTGACTGCATGGGGATTGACAGGCAGCATATTCCTGATACATACCTATGTGAACGTTGTCAGCCCAGGAGTTTGGATAAAGAGAGGGCAGTGCTACTGCAACGCCGGAAAAGAGAAAATATGTCAGATGGTGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCAACTTCAATCACTTTAACTGCTTCCAGAGTTCCCAAGGTTACTGATAAAAGAAGGAAAAAAAGTGGGGAGAAAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGGGGAGAAAGAACAAAACT
57.75
CCAAACTCCTGGGCTGACAA
60.18
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGGGGAGAAAGAACAAAACT
57.75
TGGGCTGACAACGTTCACAT
60.18
158
PCR template sequence:
TACTGATAAAAGAAGGAAAAAAAGTGGGGAGAAAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGTGTTTGGCAACATATTGACTGCATGGGGATTGACAGGCAGCATATTCCTGATACATACCTATGTGAACGTTGTCAGCCCAGGAGTTTGGATAAAGAGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-363-3p(1), mmu-miR-384-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCAAGGGTTAAGTGTTTGGCAACAT
siRNA sets
TTGCCAAACACTTAACCCT, GTTGCCAAACACTTAACCC, ATGTTGCCAAACACTTAAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKMT2E_352_ORF_1
0.8971
-0.092984141
0.188440996
348
ORF sequence:
ATGGGGATTGACAGGCAGCATATTCCTGATACATACCTATGTGAACGTTGTCAGCCCAGGAGTTTGGATAAAGAGAGGGCAGTGCTACTGCAACGCCGGAAAAGAGAAAATATGTCAGATGGTGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCAACTTCAATCACTTTAACTGCTTCCAGAGTTCCCAAGGTTACTGATAAAAGAAGGAAAAAAAGTGGGGAGAAAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGTGTTTGGCAACATATTGA
Peptide sequence:
MGIDRQHIPDTYLCERCQPRSLDKERAVLLQRRKRENMSDGDTSATESGDEVPVELYTAFQHTPTSITLTASRVPKVTDKRRKKSGEKEQNFSKCKKAFREGSRKSSRVKCLATY
IRESfinder result
IRESfinder score
0.565599426
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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