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mmu_circKmt2e_1331
Basic information of circular RNA
PanCircBase ID:
mmu_circKmt2e_1331
mm39_Chr. Position ID:
chr5|23678478|23697430|+
Gene name:
Kmt2e
Transcript:
ENSMUST00000094962.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
7,8,9,10,11,12,13,14,15
Exon/intron offsets:
0,3005,5033,5372,7728,8921,11866,12821,18787
Genomic length:
18952
Exon/intron sizes in nt:
17,33,92,31,13,11,18,10,00,00,00,000
Flanking exon/introns:
chr5:23676804-23678478|chr5:23697430-23698196
Splice length:
1331
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGGTGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCAACTTCAATCACTTTAACTGCTTCCAGAGTTCCCAAGGTTACTGATAAAAGAAGGAAAAAAAGTGGGGAGAAAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGGGTTCAGCACCAGAAATTGATCCTTCATCTGATAGTTCAAATTTTGTTTGGGAAACAAAAATCAAAGCATGGATGGATCGTTACGAAGAAGCAAATAATAACCAATATAGTGAAGGTGTTCAGAGAGAGGCACAAAGACTAGCTCAAAGACTCGGTAGTGGGAATGACAGCAAAGACATGAATAAATCAGAATTGAGTACCAACAACTCACTCTTCAGACCTCCTGTAGAGAGCCATATACAGAAGAATAAAAAAATTCTGAAATCTGCCAAAGACTTGCCTCCTGATGCACTTATCATTGAATATAGAGGGAAGTTTATGCTGAGAGAACAGTTTGAAGCAAATGGATATTTCTTTAAAAGGCCATACCCTTTTGTTTTATTCTACTCTAAGTTCCATGGGCTTGAAATGTGTGTTGATGCGAGGACTTTTGGGAATGAGGCTCGATTCATCAGGCGTTCTTGTACACCGAATGCAGAGGTGAGGCATGAAATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCATTGCCTTTGATTTTGACTACGGGAATTGCAAATACAAGGTGGACTGTGCATGCCTCAAGGAAAATCCAGAGTGCCCTGTTCTCAAGCGAAGCTCGGAATCCACAGAAAACATCAATAGTGGTTATGAGACCAGAAGGAAAAAAGGAAAAAAAGAGAAAGATACTTCAAAAGAAAAAGACATACAAAATCAGAATATGACTTTGGACTGTGAAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGTGTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAAAGGATCAGCACAGCT
59.31
TGGAGTATGCTGAAATGCAGT
57.65
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAAAGGATCAGCACAGCT
59.31
GTTGGAGTATGCTGAAATGCAGT
59.56
159
PCR template sequence:
AAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGATGGTGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCAACTTCAATCACTTTAACTGCTTCCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-384-3p(1), mmu-miR-129-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
CGAAGTGGTGCAGATGGTGATACCAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKMT2E_1331_ORF_1
0.9224
0.072868227
0.998672561
1374
ORF sequence:
ATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGATGGTGATACCAGTGCAACTGAAAGTGGTGATGAAGTTCCTGTGGAATTATATACTGCATTTCAGCATACTCCAACTTCAATCACTTTAACTGCTTCCAGAGTTCCCAAGGTTACTGATAAAAGAAGGAAAAAAAGTGGGGAGAAAGAACAAAACTTTTCAAAATGTAAAAAAGCCTTTCGTGAAGGATCTAGGAAATCATCAAGGGTTAAGGGTTCAGCACCAGAAATTGATCCTTCATCTGATAGTTCAAATTTTGTTTGGGAAACAAAAATCAAAGCATGGATGGATCGTTACGAAGAAGCAAATAATAACCAATATAGTGAAGGTGTTCAGAGAGAGGCACAAAGACTAGCTCAAAGACTCGGTAGTGGGAATGACAGCAAAGACATGAATAAATCAGAATTGAGTACCAACAACTCACTCTTCAGACCTCCTGTAGAGAGCCATATACAGAAGAATAAAAAAATTCTGAAATCTGCCAAAGACTTGCCTCCTGATGCACTTATCATTGAATATAGAGGGAAGTTTATGCTGAGAGAACAGTTTGAAGCAAATGGATATTTCTTTAAAAGGCCATACCCTTTTGTTTTATTCTACTCTAAGTTCCATGGGCTTGAAATGTGTGTTGATGCGAGGACTTTTGGGAATGAGGCTCGATTCATCAGGCGTTCTTGTACACCGAATGCAGAGGTGAGGCATGAAATTGAAGAGGGGACCATACATCTCTACATTTATTCTATACAGAGTATTCCAAAAGGGACTGAAATTACCATTGCCTTTGATTTTGACTACGGGAATTGCAAATACAAGGTGGACTGTGCATGCCTCAAGGAAAATCCAGAGTGCCCTGTTCTCAAGCGAAGCTCGGAATCCACAGAAAACATCAATAGTGGTTATGAGACCAGAAGGAAAAAAGGAAAAAAAGAGAAAGATACTTCAAAAGAAAAAGACATACAAAATCAGAATATGACTTTGGACTGTGAAGGAACAAACAACAAAATAAGGAGTCCAGAAACAAAACAGAGAAAGCTTTCTCCATTGAGGCTGTCGGTGTCAAACAACCAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAG
Peptide sequence:
MQRVAGHRRCRSGADGDTSATESGDEVPVELYTAFQHTPTSITLTASRVPKVTDKRRKKSGEKEQNFSKCKKAFREGSRKSSRVKGSAPEIDPSSDSSNFVWETKIKAWMDRYEEANNNQYSEGVQREAQRLAQRLGSGNDSKDMNKSELSTNNSLFRPPVESHIQKNKKILKSAKDLPPDALIIEYRGKFMLREQFEANGYFFKRPYPFVLFYSKFHGLEMCVDARTFGNEARFIRRSCTPNAEVRHEIEEGTIHLYIYSIQSIPKGTEITIAFDFDYGNCKYKVDCACLKENPECPVLKRSSESTENINSGYETRRKKGKKEKDTSKEKDIQNQNMTLDCEGTNNKIRSPETKQRKLSPLRLSVSNNQEPDFIDDMEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQ
IRESfinder result
IRESfinder score
0.593907227
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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