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mmu_circKmt2e_1281
Basic information of circular RNA
PanCircBase ID:
mmu_circKmt2e_1281
mm39_Chr. Position ID:
chr5|23691299|23702593|+
Gene name:
Kmt2e
Transcript:
ENSMUST00000094962.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
14,15,16,17,18,19,20
Exon/intron offsets:
0,5966,6897,9684,10102,10670,11237
Genomic length:
11294
Exon/intron sizes in nt:
99,165,309,255,145,251,57
Flanking exon/introns:
chr5:23690609-23691299|chr5:23702593-23702725
Splice length:
1281
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGAACAAGTAAAAGAAGAAACTGCTATCAAGCCAGCCGCTGCCAAAGTGAACAGAACTAAGCAGAGAAAGAGCTTTTCTCGTAGTAGGACTCACATTGGACAGCAGCGTCGGAGACACAGAACTGTCAGCATGTGTTCAGACATACCGCCATCTTCTCCTGACATCGAAGTTCTGTCACAACAGAACGAGATTGAAAATACTGTACTTGCTATAGAACCAGAAACTGAAACTGCAGTAGCAGAAATAATTCCTGAAGCGGAAGTTCCAGCACTTAATAAGTGTCCCACAAAGTACCCCAAAACAAAGAAGCACTTGGTCAATGAATGGTTAAGTGAGAAGAATGAGAAGACAGGAAAACCTTCAGACAGCCTTTCAGAAAGGCCTCTGCGCATAACTACAGATCCAGAGGTGTTAGCTACACAGCTCAATTCTTTGCCAGGTCTCACTTACAGCCCCCATGTATACTCTACTCCTAAGCATTATATTAGATTTACATCACCATTCCTTTCGGAAAAAAAGAGAAGAAAAGAAACTACTGAAAACATTTCTGGCTCATGCAAAAAGCGGTGGCTGAAGCAAGCCCTGGAGGAAGAAAATTCAACAATTTTACATAGATATCATTCACCTTGTCAGGAAAGATCCAGGAGTCCTACAGTTAATGGTGAAAACAAAAGTCCACTTCTGCTAAGCGACAGCTGCTCCCTCCCAGATTTAACCACACCACTAAAAAAGCGAAGACTTTACCAATTGTTAGATACTGCTTACTCAGAAAGCTCCACTCCTACCCCTTCACCATATGCTACACCAACTCACACAGACATCACTCCTACGGACCCAGCATTTGCCACTCCCCCACGGATCAAATCAGATGATGAAACTTACAGAAATGGTTATAAACCCATATATTCACCAGTGACCCCTGTAACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTCTCCAGAAATAAAGAGATGCACTTACAATCAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACACCAGGAAACACCATGCA
60.11
TCCTTTCTGCAATCTGTTCCTCA
59.93
172
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAGGAAACACCATGCACT
59.81
TCCTTTCTGCAATCTGTTCCTCA
59.93
170
PCR template sequence:
CCCTGTAACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTCTCCAGAAATAAAGAGATGCACTTACAATCAAGAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-298-5p(1), mmu-miR-467b-5p(1), mmu-miR-129-5p(1), mmu-miR-1904(1), mmu-miR-3105-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTACAATCAAGAGGAACCAGATTTTA
siRNA sets
TAAAATCTGGTTCCTCTTG, AAAATCTGGTTCCTCTTGA, ATCTGGTTCCTCTTGATTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKMT2E_1281_ORF_1
1.0166
0.097166119
0.999999778
2541
ORF sequence:
ATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGAACAAGTAAAAGAAGAAACTGCTATCAAGCCAGCCGCTGCCAAAGTGAACAGAACTAAGCAGAGAAAGAGCTTTTCTCGTAGTAGGACTCACATTGGACAGCAGCGTCGGAGACACAGAACTGTCAGCATGTGTTCAGACATACCGCCATCTTCTCCTGACATCGAAGTTCTGTCACAACAGAACGAGATTGAAAATACTGTACTTGCTATAGAACCAGAAACTGAAACTGCAGTAGCAGAAATAATTCCTGAAGCGGAAGTTCCAGCACTTAATAAGTGTCCCACAAAGTACCCCAAAACAAAGAAGCACTTGGTCAATGAATGGTTAAGTGAGAAGAATGAGAAGACAGGAAAACCTTCAGACAGCCTTTCAGAAAGGCCTCTGCGCATAACTACAGATCCAGAGGTGTTAGCTACACAGCTCAATTCTTTGCCAGGTCTCACTTACAGCCCCCATGTATACTCTACTCCTAAGCATTATATTAGATTTACATCACCATTCCTTTCGGAAAAAAAGAGAAGAAAAGAAACTACTGAAAACATTTCTGGCTCATGCAAAAAGCGGTGGCTGAAGCAAGCCCTGGAGGAAGAAAATTCAACAATTTTACATAGATATCATTCACCTTGTCAGGAAAGATCCAGGAGTCCTACAGTTAATGGTGAAAACAAAAGTCCACTTCTGCTAAGCGACAGCTGCTCCCTCCCAGATTTAACCACACCACTAAAAAAGCGAAGACTTTACCAATTGTTAGATACTGCTTACTCAGAAAGCTCCACTCCTACCCCTTCACCATATGCTACACCAACTCACACAGACATCACTCCTACGGACCCAGCATTTGCCACTCCCCCACGGATCAAATCAGATGATGAAACTTACAGAAATGGTTATAAACCCATATATTCACCAGTGACCCCTGTAACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTCTCCAGAAATAAAGAGATGCACTTACAATCAAGAGGAACCAGATTTTATTGATGATATGGAAGAAAAAACTCCTATTAGCAATGAAGTGGAAATGGAATCTGAGGAACAGATTGCAGAAAGGAAAAGGAAGATGACAAGAGAAGAAAGAAAAATGGAAGCAATTTTGCAGGCTTTTGCCAGACTTGAGAAGAGAGAGAAAAGACGAGAACAAGCTTTGGAAAGGATCAGCACAGCTAAGACAGAAGTTAAGCCTGAATGCAAAGAGTCGCAGGTCATCGCCGATGCCGAAGTGGTGCAGGAACAAGTAAAAGAAGAAACTGCTATCAAGCCAGCCGCTGCCAAAGTGAACAGAACTAAGCAGAGAAAGAGCTTTTCTCGTAGTAGGACTCACATTGGACAGCAGCGTCGGAGACACAGAACTGTCAGCATGTGTTCAGACATACCGCCATCTTCTCCTGACATCGAAGTTCTGTCACAACAGAACGAGATTGAAAATACTGTACTTGCTATAGAACCAGAAACTGAAACTGCAGTAGCAGAAATAATTCCTGAAGCGGAAGTTCCAGCACTTAATAAGTGTCCCACAAAGTACCCCAAAACAAAGAAGCACTTGGTCAATGAATGGTTAAGTGAGAAGAATGAGAAGACAGGAAAACCTTCAGACAGCCTTTCAGAAAGGCCTCTGCGCATAACTACAGATCCAGAGGTGTTAGCTACACAGCTCAATTCTTTGCCAGGTCTCACTTACAGCCCCCATGTATACTCTACTCCTAAGCATTATATTAGATTTACATCACCATTCCTTTCGGAAAAAAAGAGAAGAAAAGAAACTACTGAAAACATTTCTGGCTCATGCAAAAAGCGGTGGCTGAAGCAAGCCCTGGAGGAAGAAAATTCAACAATTTTACATAGATATCATTCACCTTGTCAGGAAAGATCCAGGAGTCCTACAGTTAATGGTGAAAACAAAAGTCCACTTCTGCTAAGCGACAGCTGCTCCCTCCCAGATTTAACCACACCACTAAAAAAGCGAAGACTTTACCAATTGTTAGATACTGCTTACTCAGAAAGCTCCACTCCTACCCCTTCACCATATGCTACACCAACTCACACAGACATCACTCCTACGGACCCAGCATTTGCCACTCCCCCACGGATCAAATCAGATGATGAAACTTACAGAAATGGTTATAAACCCATATATTCACCAGTGACCCCTGTAACCCCTGGCACACCAGGAAACACCATGCACTTTGAGAATATTTCTTCCCCAGAAAGTTCTCCAGAAATAAAGAGATGCACTTACAATCAAGAG
Peptide sequence:
MEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQEQVKEETAIKPAAAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIPPSSPDIEVLSQQNEIENTVLAIEPETETAVAEIIPEAEVPALNKCPTKYPKTKKHLVNEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKKRRKETTENISGSCKKRWLKQALEEENSTILHRYHSPCQERSRSPTVNGENKSPLLLSDSCSLPDLTTPLKKRRLYQLLDTAYSESSTPTPSPYATPTHTDITPTDPAFATPPRIKSDDETYRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRCTYNQEEPDFIDDMEEKTPISNEVEMESEEQIAERKRKMTREERKMEAILQAFARLEKREKRREQALERISTAKTEVKPECKESQVIADAEVVQEQVKEETAIKPAAAKVNRTKQRKSFSRSRTHIGQQRRRHRTVSMCSDIPPSSPDIEVLSQQNEIENTVLAIEPETETAVAEIIPEAEVPALNKCPTKYPKTKKHLVNEWLSEKNEKTGKPSDSLSERPLRITTDPEVLATQLNSLPGLTYSPHVYSTPKHYIRFTSPFLSEKKRRKETTENISGSCKKRWLKQALEEENSTILHRYHSPCQERSRSPTVNGENKSPLLLSDSCSLPDLTTPLKKRRLYQLLDTAYSESSTPTPSPYATPTHTDITPTDPAFATPPRIKSDDETYRNGYKPIYSPVTPVTPGTPGNTMHFENISSPESSPEIKRCTYNQE
IRESfinder result
IRESfinder score
0.596533098
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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