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mmu_circKlhl7_1062
Basic information of circular RNA
PanCircBase ID:
mmu_circKlhl7_1062
mm39_Chr. Position ID:
chr5|24332977|24362089|+
Gene name:
Klhl7
Transcript:
ENSMUST00000030841.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
4,5,6,7,8,9
Exon/intron offsets:
0,6984,10326,13171,21145,28910
Genomic length:
29112
Exon/intron sizes in nt:
1,25,17,61,75,14,32,41,000
Flanking exon/introns:
chr5:24332760-24332977|chr5:24362089-24363536
Splice length:
1062
Other database IDs:
circAtlas: mmu-Klhl7_0021
Human Ortholog IDs:
Mature spliced Sequence:
AATTTCTGTGAATAGCAACAATGTTCAGTCTTTGTTAGATGCAGCAAATCAGTACCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAACAAGTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTTGCAGAGTGTCTGGACTGTCCTGAACTGAAAGCTACAGCTGATGACTTCATTCATCAGCATTTCACTGAGGTTTACAAAACAGATGAATTCCTGCAGCTTGATGTCAAGCGAGTGACACATCTTCTCAGCCAGGACACCCTGACTGTGAGAGCAGAGGACCAGGTTTATGACGCTGCCGTGAGGTGGTTGAAATATGATGAGCCTAACCGCCAGCCATTCATGGTTGATATCCTTGCTAAAGTCAGGTTTCCTCTTATATCGAAGAATTTCCTAAGTAAAACAGTACAAGCTGAACCACTTATTCAAGATAATCCTGAATGCCTTAAAATGGTGATAAGTGGAATGAGGTACCATCTGCTGTCTCCTGAGGATCGAGAAGAACTTGCAGGTGGCACACGGCCTAGAAGAAAGAAACATGATTACCGCATAGCTCTGTTTGGAGGCTCCCAACCACAGTCCTGTAGATATTTTAACCCAAAGGATTACAGTTGGACAGACATTCGCTGCCCCTTTGAAAAACGAAGAGATGCAGCATGTGTGTTTTGGGACAATGTAGTATACATTTTGGGAGGTTCTCAGCTTTTCCCCATAAAGCGAATGGATTGCTACAATGTTGTAAAGGATAGCTGGTATTCCAAACTGGGCCCTCCAACACCTCGAGACAGCCTTGCTGCCTGTGCAGCAGAAGGCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCGGCCCTGTATTTGTTTGAATGCTATGATACAAGGACCGAGAGTTGGCACACGAAGCCCAGCATGCTGACTCAGCGCTGCAGCCATGGGATGGTGGAGGCCAATGGCCTCATCTATGTTTGTGGTGGAAGCTTAGGAAACAATGTTTCTGGGAGAGTGCTTAGTTCCTGTGAAGTGTATGATCCTGCCACAGAAAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTTGTGGTGGAAGCTTAGGA
59.83
TCTGGTACTGATTTGCTGCA
57.14
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCAATGGCCTCATCTATGT
57.72
TCTGGTACTGATTTGCTGCA
57.14
157
PCR template sequence:
AGGCCAATGGCCTCATCTATGTTTGTGGTGGAAGCTTAGGAAACAATGTTTCTGGGAGAGTGCTTAGTTCCTGTGAAGTGTATGATCCTGCCACAGAAACAATTTCTGTGAATAGCAACAATGTTCAGTCTTTGTTAGATGCAGCAAATCAGTACCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-150-5p(1), mmu-miR-34a-5p(1), mmu-miR-203-3p(1), mmu-miR-3470b(1), mmu-miR-1904(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTGCCACAGAAACAATTTCTGTGAAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKLHL7_1062_ORF_1
0.9228
0.096060251
0.999990655
2046
ORF sequence:
ATGTGTGTTGATTTTTTGAAAGAACAAGTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTTGCAGAGTGTCTGGACTGTCCTGAACTGAAAGCTACAGCTGATGACTTCATTCATCAGCATTTCACTGAGGTTTACAAAACAGATGAATTCCTGCAGCTTGATGTCAAGCGAGTGACACATCTTCTCAGCCAGGACACCCTGACTGTGAGAGCAGAGGACCAGGTTTATGACGCTGCCGTGAGGTGGTTGAAATATGATGAGCCTAACCGCCAGCCATTCATGGTTGATATCCTTGCTAAAGTCAGGTTTCCTCTTATATCGAAGAATTTCCTAAGTAAAACAGTACAAGCTGAACCACTTATTCAAGATAATCCTGAATGCCTTAAAATGGTGATAAGTGGAATGAGGTACCATCTGCTGTCTCCTGAGGATCGAGAAGAACTTGCAGGTGGCACACGGCCTAGAAGAAAGAAACATGATTACCGCATAGCTCTGTTTGGAGGCTCCCAACCACAGTCCTGTAGATATTTTAACCCAAAGGATTACAGTTGGACAGACATTCGCTGCCCCTTTGAAAAACGAAGAGATGCAGCATGTGTGTTTTGGGACAATGTAGTATACATTTTGGGAGGTTCTCAGCTTTTCCCCATAAAGCGAATGGATTGCTACAATGTTGTAAAGGATAGCTGGTATTCCAAACTGGGCCCTCCAACACCTCGAGACAGCCTTGCTGCCTGTGCAGCAGAAGGCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCGGCCCTGTATTTGTTTGAATGCTATGATACAAGGACCGAGAGTTGGCACACGAAGCCCAGCATGCTGACTCAGCGCTGCAGCCATGGGATGGTGGAGGCCAATGGCCTCATCTATGTTTGTGGTGGAAGCTTAGGAAACAATGTTTCTGGGAGAGTGCTTAGTTCCTGTGAAGTGTATGATCCTGCCACAGAAACAATTTCTGTGAATAGCAACAATGTTCAGTCTTTGTTAGATGCAGCAAATCAGTACCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAACAAGTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTTGCAGAGTGTCTGGACTGTCCTGAACTGAAAGCTACAGCTGATGACTTCATTCATCAGCATTTCACTGAGGTTTACAAAACAGATGAATTCCTGCAGCTTGATGTCAAGCGAGTGACACATCTTCTCAGCCAGGACACCCTGACTGTGAGAGCAGAGGACCAGGTTTATGACGCTGCCGTGAGGTGGTTGAAATATGATGAGCCTAACCGCCAGCCATTCATGGTTGATATCCTTGCTAAAGTCAGGTTTCCTCTTATATCGAAGAATTTCCTAAGTAAAACAGTACAAGCTGAACCACTTATTCAAGATAATCCTGAATGCCTTAAAATGGTGATAAGTGGAATGAGGTACCATCTGCTGTCTCCTGAGGATCGAGAAGAACTTGCAGGTGGCACACGGCCTAGAAGAAAGAAACATGATTACCGCATAGCTCTGTTTGGAGGCTCCCAACCACAGTCCTGTAGATATTTTAACCCAAAGGATTACAGTTGGACAGACATTCGCTGCCCCTTTGAAAAACGAAGAGATGCAGCATGTGTGTTTTGGGACAATGTAGTATACATTTTGGGAGGTTCTCAGCTTTTCCCCATAAAGCGAATGGATTGCTACAATGTTGTAAAGGATAGCTGGTATTCCAAACTGGGCCCTCCAACACCTCGAGACAGCCTTGCTGCCTGTGCAGCAGAAGGCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCGGCCCTGTATTTGTTTGAATGCTATGATACAAGGACCGAGAGTTGGCACACGAAGCCCAGCATGCTGACTCAGCGCTGCAGCCATGGGATGGTGGAGGCCAATGGCCTCATCTATGTTTGTGGTGGAAGCTTAGGAAACAATGTTTCTGGGAGAGTGCTTAGTTCCTGTGAAGTGTATGATCCTGCCACAGAA
Peptide sequence:
MCVDFLKEQVDASNCLGISVLAECLDCPELKATADDFIHQHFTEVYKTDEFLQLDVKRVTHLLSQDTLTVRAEDQVYDAAVRWLKYDEPNRQPFMVDILAKVRFPLISKNFLSKTVQAEPLIQDNPECLKMVISGMRYHLLSPEDREELAGGTRPRRKKHDYRIALFGGSQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIKRMDCYNVVKDSWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQRCSHGMVEANGLIYVCGGSLGNNVSGRVLSSCEVYDPATETISVNSNNVQSLLDAANQYQIEPVKKMCVDFLKEQVDASNCLGISVLAECLDCPELKATADDFIHQHFTEVYKTDEFLQLDVKRVTHLLSQDTLTVRAEDQVYDAAVRWLKYDEPNRQPFMVDILAKVRFPLISKNFLSKTVQAEPLIQDNPECLKMVISGMRYHLLSPEDREELAGGTRPRRKKHDYRIALFGGSQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIKRMDCYNVVKDSWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQRCSHGMVEANGLIYVCGGSLGNNVSGRVLSSCEVYDPATE
IRESfinder result
IRESfinder score
0.587053357
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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