top of page
mmu_circKlhl32_473
Basic information of circular RNA
PanCircBase ID:
mmu_circKlhl32_473
mm39_Chr. Position ID:
chr4|24708952|24800890|-
Gene name:
Klhl32
Transcript:
ENSMUST00000108218.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,4,3,2
Exon/intron offsets:
0,34905,83664,91853
Genomic length:
91938
Exon/intron sizes in nt:
99,108,181,85
Flanking exon/introns:
chr4:24682270-24708952|chr4:24800890-24850737
Splice length:
473
Other database IDs:
circAtlas: mmu-Klhl32_0008
circBase: hsa_circ_0132706, circAtlas: hsa-KLHL32_0002, CIRCpedia: HSA_CIRCpedia_146742
Human Ortholog IDs:
Mature spliced Sequence:
CTGGAATGCTTGCGGATTCTTCTGAAACCTTCTAAGCCTGACTCTGACGACCCAGCTTGAGGATGCCGTCTGAACACTGCCTCAGTATTCAAGAAATGCTGACGGGCCAGAGGCTGTGCCACTCGGAAACTCACAATGACACGGTCCTGGCGGCGCTGAATCAGCAAAGAAGTGATGGCATTCTCTGCGACGTGACCCTGATCGCCGAGGAACAGAAATTCCACGCTCACAAGGCGGTCCTAGCAGCCTGCAGTGACTACTTCCGGGCAATGTTCAGTCTTTGTATGGTGGAAAGTGGAGCCGATGAAGTAAATTTACGTGGTGTGACCAGCCTGGGCTTAAAGCAGGCTCTGGAGTTCGCATATACAGGACAGATTTTGTTGGAGCCAGGAGTGATCCAGGATGTGTTAGCAGCTGGCAGTCACCTACAGCTGTTGGAACTTCTCAATCTGTGCTCCCACTATCTCATCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCAGGAGTGATCCAGGAT
60.03
ACTGAGGCAGTGTTCAGACG
59.97
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCAGGAGTGATCCAGGAT
60.03
CAGTGTTCAGACGGCATCCT
60.04
168
PCR template sequence:
GATTTTGTTGGAGCCAGGAGTGATCCAGGATGTGTTAGCAGCTGGCAGTCACCTACAGCTGTTGGAACTTCTCAATCTGTGCTCCCACTATCTCATCCAGCTGGAATGCTTGCGGATTCTTCTGAAACCTTCTAAGCCTGACTCTGACGACCCAGCTTGAGGATGCCGTCTGAACACTGCCTCAGTATTCAAGAAATGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-200c-3p(1), mmu-miR-320-3p(1), mmu-let-7d-5p(1), mmu-miR-34b-5p(1), mmu-miR-705(1), mmu-miR-673-5p(1), mmu-miR-3065-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
CTATCTCATCCAGCTGGAATGCTTGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKLHL32_473_ORF_1
1.0926
0.288671871
0.793436304
471
ORF sequence:
ATGCCGTCTGAACACTGCCTCAGTATTCAAGAAATGCTGACGGGCCAGAGGCTGTGCCACTCGGAAACTCACAATGACACGGTCCTGGCGGCGCTGAATCAGCAAAGAAGTGATGGCATTCTCTGCGACGTGACCCTGATCGCCGAGGAACAGAAATTCCACGCTCACAAGGCGGTCCTAGCAGCCTGCAGTGACTACTTCCGGGCAATGTTCAGTCTTTGTATGGTGGAAAGTGGAGCCGATGAAGTAAATTTACGTGGTGTGACCAGCCTGGGCTTAAAGCAGGCTCTGGAGTTCGCATATACAGGACAGATTTTGTTGGAGCCAGGAGTGATCCAGGATGTGTTAGCAGCTGGCAGTCACCTACAGCTGTTGGAACTTCTCAATCTGTGCTCCCACTATCTCATCCAGCTGGAATGCTTGCGGATTCTTCTGAAACCTTCTAAGCCTGACTCTGACGACCCAGCTTGA
Peptide sequence:
MPSEHCLSIQEMLTGQRLCHSETHNDTVLAALNQQRSDGILCDVTLIAEEQKFHAHKAVLAACSDYFRAMFSLCMVESGADEVNLRGVTSLGLKQALEFAYTGQILLEPGVIQDVLAAGSHLQLLELLNLCSHYLIQLECLRILLKPSKPDSDDPA
IRESfinder result
IRESfinder score
0.340565358
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page