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mmu_circKlhl32_289
Basic information of circular RNA
PanCircBase ID:
mmu_circKlhl32_289
mm39_Chr. Position ID:
chr4|24743857|24792797|-
Gene name:
Klhl32
Transcript:
ENSMUST00000108214.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,48759
Genomic length:
48940
Exon/intron sizes in nt:
108,181
Flanking exon/introns:
chr4:24709051-24743857|chr4:24792797-24800805
Splice length:
289
Other database IDs:
circAtlas: hsa-KLHL32_0011, CIRCpedia: HSA_CIRCpedia_146743
Human Ortholog IDs:
Mature spliced Sequence:
TATTCAAGAAATGCTGACGGGCCAGAGGCTGTGCCACTCGGAAACTCACAATGACACGGTCCTGGCGGCGCTGAATCAGCAAAGAAGTGATGGCATTCTCTGCGACGTGACCCTGATCGCCGAGGAACAGAAATTCCACGCTCACAAGGCGGTCCTAGCAGCCTGCAGTGACTACTTCCGGGCAATGTTCAGTCTTTGTATGGTGGAAAGTGGAGCCGATGAAGTAAATTTACGTGGTGTGACCAGCCTGGGCTTAAAGCAGGCTCTGGAGTTCGCATATACAGGACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGAAAGTGGAGCCGATGA
60.04
CCAGGACCGTGTCATTGTGA
59.97
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TATGGTGGAAAGTGGAGCCG
59.75
CCAGGACCGTGTCATTGTGA
59.97
156
PCR template sequence:
CAGTCTTTGTATGGTGGAAAGTGGAGCCGATGAAGTAAATTTACGTGGTGTGACCAGCCTGGGCTTAAAGCAGGCTCTGGAGTTCGCATATACAGGACAGTATTCAAGAAATGCTGACGGGCCAGAGGCTGTGCCACTCGGAAACTCACAATGACACGGTCCTGGCGGCGCTGAATCAGCAAAGAAGTGATGGCATTCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATATACAGGACAGTATTCAAGAAATG
siRNA sets
TTTCTTGAATACTGTCCTG, CATTTCTTGAATACTGTCC, ATTTCTTGAATACTGTCCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKLHL32_289_ORF_1
0.9363
0.284047483
0.51222902
333
ORF sequence:
ATGCTGACGGGCCAGAGGCTGTGCCACTCGGAAACTCACAATGACACGGTCCTGGCGGCGCTGAATCAGCAAAGAAGTGATGGCATTCTCTGCGACGTGACCCTGATCGCCGAGGAACAGAAATTCCACGCTCACAAGGCGGTCCTAGCAGCCTGCAGTGACTACTTCCGGGCAATGTTCAGTCTTTGTATGGTGGAAAGTGGAGCCGATGAAGTAAATTTACGTGGTGTGACCAGCCTGGGCTTAAAGCAGGCTCTGGAGTTCGCATATACAGGACAGTATTCAAGAAATGCTGACGGGCCAGAGGCTGTGCCACTCGGAAACTCACAATGA
Peptide sequence:
MLTGQRLCHSETHNDTVLAALNQQRSDGILCDVTLIAEEQKFHAHKAVLAACSDYFRAMFSLCMVESGADEVNLRGVTSLGLKQALEFAYTGQYSRNADGPEAVPLGNSQ
IRESfinder result
IRESfinder score
0.224761305
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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