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mmu_circKlhl2_270
Basic information of circular RNA
PanCircBase ID:
mmu_circKlhl2_270
mm39_Chr. Position ID:
chr8|65202065|65202857|-
Gene name:
Klhl2
Transcript:
ENSMUST00000034017.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
13,12
Exon/intron offsets:
0,663
Genomic length:
792
Exon/intron sizes in nt:
1,41,129
Flanking exon/introns:
chr8:65196132-65202065|chr8:65202857-65204762
Splice length:
270
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGCTCTATGCCGTGGGGGGTTACGATGGAGCATCGCGCCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACTGCAAACGAGTGGACTTACATAGCAGAAATGAGCACCAGACGGAGCGGAGCAGGTGTTGGTGTGTTAAACAACTTATTATATGCTGTAGGAGGTCATGATGGTCCTTTAGTACGAAAAAGTGTTGAAGTATATGACCCCACCACAAATGCCTGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACCACAAATGCCTGGAGA
60.25
GTCCACTCGTTTGCAGTAGC
59.21
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCACAAATGCCTGGAGACA
59.52
GTCCACTCGTTTGCAGTAGC
59.21
146
PCR template sequence:
CATGATGGTCCTTTAGTACGAAAAAGTGTTGAAGTATATGACCCCACCACAAATGCCTGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCAGGTTTGCTCTATGCCGTGGGGGGTTACGATGGAGCATCGCGCCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACTGCAAACGAGTGGACTTACATAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AGAAGAAATGCAGGTTTGCTCTATGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKLHL2_270_ORF_1
1.0959
0.009583395
0.490137225
435
ORF sequence:
ATGAGCACCAGACGGAGCGGAGCAGGTGTTGGTGTGTTAAACAACTTATTATATGCTGTAGGAGGTCATGATGGTCCTTTAGTACGAAAAAGTGTTGAAGTATATGACCCCACCACAAATGCCTGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCAGGTTTGCTCTATGCCGTGGGGGGTTACGATGGAGCATCGCGCCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACTGCAAACGAGTGGACTTACATAGCAGAAATGAGCACCAGACGGAGCGGAGCAGGTGTTGGTGTGTTAAACAACTTATTATATGCTGTAGGAGGTCATGATGGTCCTTTAGTACGAAAAAGTGTTGAAGTATATGACCCCACCACAAATGCCTGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCA
Peptide sequence:
MSTRRSGAGVGVLNNLLYAVGGHDGPLVRKSVEVYDPTTNAWRQVADMNMCRRNAGLLYAVGGYDGASRQCLSTVECYNATANEWTYIAEMSTRRSGAGVGVLNNLLYAVGGHDGPLVRKSVEVYDPTTNAWRQVADMNMCRRNA
IRESfinder result
IRESfinder score
0.234317865
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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