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mmu_circKlhl22_719
Basic information of circular RNA
PanCircBase ID:
mmu_circKlhl22_719
mm39_Chr. Position ID:
chr16|17594265|17594984|+
Gene name:
Klhl22
Transcript:
ENSMUST00000165790.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
4
Exon/intron offsets:
0
Genomic length:
719
Exon/intron sizes in nt:
719
Flanking exon/introns:
chr16:17589733-17594265|chr16:17594984-17602066
Splice length:
719
Other database IDs:
circAtlas: mmu-Klhl22_0002
circBase: hsa_circ_0002256, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_35989
Human Ortholog IDs:
Mature spliced Sequence:
ATACCAGAAATTATCCACTTCTGCTGTGATTTCCTCATGTCATGGGTAGATGAGGAGAATATCCTTGATGTCTACCGGCTAGCAGACCTCTTTGACTTGAACCATCTGACCCAACAGCTAGATACCTACATCCTCAAAAACTTTGTGGCCTTCTCCAGAACTGACAAGTACCGCCAGCTTCCCTTGGAGAAGGTCTACTCTCTCCTCAGCAGCAATCGCCTGGAGGTTTCCTGTGAGACTGAAGTGTATGAAGGGGCCCTGCTCTACCATTACTCCCTAGAGCAGGTGCAGGCTGACCAGATCTCACTGAATGAGCCCCCCAAGCTCCTAGAGACTGTGCGCTTTCCCCTGATGGAAGCTGAGGTCCTTCAGCGGCTGCATGACAAGCTTGGTCCTAGCCCACTGAGGGACACAGTGGCCAGTGCCCTTATGTACCACCGGAATGAGATCCTGCAGCCCAGCCTTCAGGGCCCACAGACAGAGCTTCGCTCGGACTTCCAGTGTGTTGTGGGCTTTGGGGGTATTCACTCCACACCGTCTACAATCCTCAGTGACCAGGCCAAGTACCTGAATCCTCTACTGGGAGAGTGGAAACACTTCACTGCCTCTCTGGCGCCCCGAATGTCCAATCAGGGCATTGCAGTGCTCAACAACTTCGTGTACTTAATTGGAGGGGACAATAATGTCCAAGGATTTCGAGCTGAGTCCCGATGCTGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCATTGCAGTGCTCAACAA
59.97
AAGAGGTCTGCTAGCCGGTA
60.03
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCATTGCAGTGCTCAACAA
59.97
CAAAGAGGTCTGCTAGCCGG
60.46
180
PCR template sequence:
GAATGTCCAATCAGGGCATTGCAGTGCTCAACAACTTCGTGTACTTAATTGGAGGGGACAATAATGTCCAAGGATTTCGAGCTGAGTCCCGATGCTGGAGATACCAGAAATTATCCACTTCTGCTGTGATTTCCTCATGTCATGGGTAGATGAGGAGAATATCCTTGATGTCTACCGGCTAGCAGACCTCTTTGACTTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-205-5p(1), mmu-miR-382-5p(1), mmu-miR-362-5p(1), mmu-miR-452-3p(1), mmu-miR-3061-5p(1), mmu-miR-193b-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCGATGCTGGAGATACCAGAAATTA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKLHL22_719_ORF_1
1.1264
0.33393514
0.969694435
732
ORF sequence:
ATGTCATGGGTAGATGAGGAGAATATCCTTGATGTCTACCGGCTAGCAGACCTCTTTGACTTGAACCATCTGACCCAACAGCTAGATACCTACATCCTCAAAAACTTTGTGGCCTTCTCCAGAACTGACAAGTACCGCCAGCTTCCCTTGGAGAAGGTCTACTCTCTCCTCAGCAGCAATCGCCTGGAGGTTTCCTGTGAGACTGAAGTGTATGAAGGGGCCCTGCTCTACCATTACTCCCTAGAGCAGGTGCAGGCTGACCAGATCTCACTGAATGAGCCCCCCAAGCTCCTAGAGACTGTGCGCTTTCCCCTGATGGAAGCTGAGGTCCTTCAGCGGCTGCATGACAAGCTTGGTCCTAGCCCACTGAGGGACACAGTGGCCAGTGCCCTTATGTACCACCGGAATGAGATCCTGCAGCCCAGCCTTCAGGGCCCACAGACAGAGCTTCGCTCGGACTTCCAGTGTGTTGTGGGCTTTGGGGGTATTCACTCCACACCGTCTACAATCCTCAGTGACCAGGCCAAGTACCTGAATCCTCTACTGGGAGAGTGGAAACACTTCACTGCCTCTCTGGCGCCCCGAATGTCCAATCAGGGCATTGCAGTGCTCAACAACTTCGTGTACTTAATTGGAGGGGACAATAATGTCCAAGGATTTCGAGCTGAGTCCCGATGCTGGAGATACCAGAAATTATCCACTTCTGCTGTGATTTCCTCATGTCATGGGTAG
Peptide sequence:
MSWVDEENILDVYRLADLFDLNHLTQQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCETEVYEGALLYHYSLEQVQADQISLNEPPKLLETVRFPLMEAEVLQRLHDKLGPSPLRDTVASALMYHRNEILQPSLQGPQTELRSDFQCVVGFGGIHSTPSTILSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNFVYLIGGDNNVQGFRAESRCWRYQKLSTSAVISSCHG
IRESfinder result
IRESfinder score
0.57106554
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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