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mmu_circKiz_216
Basic information of circular RNA
PanCircBase ID:
mmu_circKiz_216
mm39_Chr. Position ID:
chr2|146784836|146794984|+
Gene name:
Kiz
Transcript:
ENSMUST00000099278.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
9,10,11
Exon/intron offsets:
0,7606,10061
Genomic length:
10148
Exon/intron sizes in nt:
63,66,87
Flanking exon/introns:
chr2:146784095-146784836|chr2:146794984-146798119
Splice length:
216
Other database IDs:
circAtlas: mmu-Kiz_0013
Human Ortholog IDs:
Mature spliced Sequence:
AAGTTTCAAGTGGCTGTGGAGATGAGAGCAAGGAAGAAAGCATAGCTGCGGCCCCAGGCACAGGACACAGAGAGGCGGATCTAGTACCCTCAGAACTGTTTCAGTTCAGCTTGGGAGCAGCCATGAAAGAAACAAAAGCCTACCAGTTACTGAAGAAGTCTACCCTTCAGGACAATTCAAATCAAGCTGAAGAGAGATTTGAAAGTCAGTTTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGCCATGAAAGAAACAAAAGC
60.06
CTCTCTGTGTCCTGTGCCTG
60.04
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCATGAAAGAAACAAAAGCCT
59.61
CTCTCTGTGTCCTGTGCCTG
60.04
172
PCR template sequence:
GCAGCCATGAAAGAAACAAAAGCCTACCAGTTACTGAAGAAGTCTACCCTTCAGGACAATTCAAATCAAGCTGAAGAGAGATTTGAAAGTCAGTTTTCAGAAGTTTCAAGTGGCTGTGGAGATGAGAGCAAGGAAGAAAGCATAGCTGCGGCCCCAGGCACAGGACACAGAGAGGCGGATCTAGTACCCTCAGAACTGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-335-3p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTCAGTTTTCAGAAGTTTCAAGTGG
siRNA sets
TTGAAACTTCTGAAAACTG, CTTGAAACTTCTGAAAACT, AAACTTCTGAAAACTGACT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIZ_216_ORF_1
0.8072
0.199721814
0.330546858
309
ORF sequence:
ATGAAAGAAACAAAAGCCTACCAGTTACTGAAGAAGTCTACCCTTCAGGACAATTCAAATCAAGCTGAAGAGAGATTTGAAAGTCAGTTTTCAGAAGTTTCAAGTGGCTGTGGAGATGAGAGCAAGGAAGAAAGCATAGCTGCGGCCCCAGGCACAGGACACAGAGAGGCGGATCTAGTACCCTCAGAACTGTTTCAGTTCAGCTTGGGAGCAGCCATGAAAGAAACAAAAGCCTACCAGTTACTGAAGAAGTCTACCCTTCAGGACAATTCAAATCAAGCTGAAGAGAGATTTGAAAGTCAGTTTTCA
Peptide sequence:
MKETKAYQLLKKSTLQDNSNQAEERFESQFSEVSSGCGDESKEESIAAAPGTGHREADLVPSELFQFSLGAAMKETKAYQLLKKSTLQDNSNQAEERFESQFS
IRESfinder result
IRESfinder score
0.59057144
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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