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mmu_circKif5b_1728
Basic information of circular RNA
PanCircBase ID:
mmu_circKif5b_1728
mm39_Chr. Position ID:
chr18|6210699|6223691|-
Gene name:
Kif5b
Transcript:
ENSMUST00000025083.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
23,22,21,20,19,18,17,16,15,14,13,12,11,10
Exon/intron offsets:
0,324,492,1817,2487,2693,3270,3812,5523,6041,9524,10102,12019,12846
Genomic length:
12992
Exon/intron sizes in nt:
1,05,72,61,10,21,10,62,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr18:6209216-6210699|chr18:6223691-6223970
Splice length:
1728
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACCTATGTTCCTTATCGAGATAGTAAAATGACCAGAATTCTTCAAGATTCATTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTCCATCATCATACAATGAGTCTGAGACAAAGTCAACACTCCTCTTTGGTCAAAGGGCCAAAACAATTAAGAACACAGTCTGTGTCAATGTAGAGTTAACTGCGGAGCAGTGGAAAAAGAAGTATGAAAAAGAAAAGGAAAAAAATAAAACTCTACGGAACACTATTCAGTGGCTGGAAAACGAGCTAAACCGTTGGCGTAACGGGGAGACAGTGCCTATTGATGAGCAGTTTGACAAAGAGAAAGCTAATTTGGAAGCCTTCACAGCGGATAAAGATATTGCTATTACCAGTGATAAACCAGCTGCTGCAGTCGGAATGGCTGGTAGTTTTACCGATGCTGAAAGAAGAAAGTGTGAAGAAGAACTTGCTAAATTGTATAAACAACTTGATGACAAGGATGAAGAGATTAACCAACAAAGCCAATTGGTAGAGAAATTGAAGACACAAATGCTGGATCAGGAAGAGCTTCTGGCATCAACCAGAAGGGATCAAGATAATATGCAAGCTGAACTGAATCGCCTCCAAGCAGAAAATGATGCTTCTAAAGAAGAAGTCAAAGAAGTTTTACAGGCCTTAGAGGAACTGGCTGTTAATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACAAAGGAATATGAATTGCTTAGTGATGAATTGAATCAAAAATCTGCAACTTTAGCAAGTATTGATGCTGAGCTTCAGAAGCTGAAGGAAATGACCAACCACCAGAAGAAACGAGCAGCTGAAATGATGGCATCATTATTAAAAGACCTTGCAGAAATAGGAATTGCTGTGGGGAATAACGATGTGAAGCAACCAGAAGGAACTGGTATGATAGATGAAGAGTTTACTGTTGCAAGACTCTACATTAGCAAAATGAAATCAGAAGTAAAGACCATGGTGAAACGCTGCAAACAGCTAGAAAGCACACAGACTGAGAGCAACAAAAAAATGGAAGAAAATGAGAAAGAGTTAGCAGCATGCCAGCTTCGGATCTCCCAACATGAAGCCAAAATCAAGTCACTGACTGAGTACCTTCAGAATGTAGAACAAAAGAAGAGGCAGCTGGAGGAGTCTGTTGATTCCCTTGGTGAGGAGCTAGTCCAACTCCGAGCACAAGAGAAAGTCCATGAAATGGAAAAAGAGCACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCTGTTGAGCAGCAGATCCAGAGTCACAGAGAAACCCACCAAAAACAAATCAGTAGTTTGCGAGATGAAGTGGAGGCAAAGGAAAAGCTAATCACTGACCTCCAAGACCAAAACCAGAAGATGGTGTTGGAGCAGGAACGGCTAAGGGTGGAGCATGAGAGGCTGAAGGCTACAGACCAAGAGAAGAGCAGGAAGCTGCACGAGCTCACGGTTATGCAAGACAGACGAGAACAAGCAAGACAAGACTTGAAGGGTTTGGAGGAGACCGTGGCAAAAGAACTTCAGACTTTACACAACCTGCGTAAGCTCTTTGTTCAGGACTTGGCTACCAGGGTGAAAAAGAGCGCCGAGGTCGACTCTGACGACACTGGCGGCAGTGCTGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAACAGCTCACCAAAGTGCACAAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACTCTGACGACACTGGCG
60.44
GTGGTCCTACAGTTGCCACC
60.61
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGACTCTGACGACACTGGC
60.44
GTGGTCCTACAGTTGCCACC
60.61
168
PCR template sequence:
CGAGGTCGACTCTGACGACACTGGCGGCAGTGCTGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAACAGCTCACCAAAGTGCACAAGCAGACCTATGTTCCTTATCGAGATAGTAAAATGACCAGAATTCTTCAAGATTCATTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTCCATCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-431-5p(2), mmu-miR-29b-1-5p(2), mmu-miR-1904(2), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-199a-5p(1), mmu-miR-122-5p(1), mmu-miR-15b-5p(1), mmu-miR-136-5p(1), mmu-miR-127-5p(1), mmu-miR-382-5p(1), mmu-miR-362-5p(1), mmu-miR-324-3p(1), mmu-miR-3105-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTGCACAAGCAGACCTATGTTCCTT
siRNA sets
AAGGAACATAGGTCTGCTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF5B_1728_ORF_1
1.0936
0.25811101
1
3429
ORF sequence:
ATGACCAGAATTCTTCAAGATTCATTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTCCATCATCATACAATGAGTCTGAGACAAAGTCAACACTCCTCTTTGGTCAAAGGGCCAAAACAATTAAGAACACAGTCTGTGTCAATGTAGAGTTAACTGCGGAGCAGTGGAAAAAGAAGTATGAAAAAGAAAAGGAAAAAAATAAAACTCTACGGAACACTATTCAGTGGCTGGAAAACGAGCTAAACCGTTGGCGTAACGGGGAGACAGTGCCTATTGATGAGCAGTTTGACAAAGAGAAAGCTAATTTGGAAGCCTTCACAGCGGATAAAGATATTGCTATTACCAGTGATAAACCAGCTGCTGCAGTCGGAATGGCTGGTAGTTTTACCGATGCTGAAAGAAGAAAGTGTGAAGAAGAACTTGCTAAATTGTATAAACAACTTGATGACAAGGATGAAGAGATTAACCAACAAAGCCAATTGGTAGAGAAATTGAAGACACAAATGCTGGATCAGGAAGAGCTTCTGGCATCAACCAGAAGGGATCAAGATAATATGCAAGCTGAACTGAATCGCCTCCAAGCAGAAAATGATGCTTCTAAAGAAGAAGTCAAAGAAGTTTTACAGGCCTTAGAGGAACTGGCTGTTAATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACAAAGGAATATGAATTGCTTAGTGATGAATTGAATCAAAAATCTGCAACTTTAGCAAGTATTGATGCTGAGCTTCAGAAGCTGAAGGAAATGACCAACCACCAGAAGAAACGAGCAGCTGAAATGATGGCATCATTATTAAAAGACCTTGCAGAAATAGGAATTGCTGTGGGGAATAACGATGTGAAGCAACCAGAAGGAACTGGTATGATAGATGAAGAGTTTACTGTTGCAAGACTCTACATTAGCAAAATGAAATCAGAAGTAAAGACCATGGTGAAACGCTGCAAACAGCTAGAAAGCACACAGACTGAGAGCAACAAAAAAATGGAAGAAAATGAGAAAGAGTTAGCAGCATGCCAGCTTCGGATCTCCCAACATGAAGCCAAAATCAAGTCACTGACTGAGTACCTTCAGAATGTAGAACAAAAGAAGAGGCAGCTGGAGGAGTCTGTTGATTCCCTTGGTGAGGAGCTAGTCCAACTCCGAGCACAAGAGAAAGTCCATGAAATGGAAAAAGAGCACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCTGTTGAGCAGCAGATCCAGAGTCACAGAGAAACCCACCAAAAACAAATCAGTAGTTTGCGAGATGAAGTGGAGGCAAAGGAAAAGCTAATCACTGACCTCCAAGACCAAAACCAGAAGATGGTGTTGGAGCAGGAACGGCTAAGGGTGGAGCATGAGAGGCTGAAGGCTACAGACCAAGAGAAGAGCAGGAAGCTGCACGAGCTCACGGTTATGCAAGACAGACGAGAACAAGCAAGACAAGACTTGAAGGGTTTGGAGGAGACCGTGGCAAAAGAACTTCAGACTTTACACAACCTGCGTAAGCTCTTTGTTCAGGACTTGGCTACCAGGGTGAAAAAGAGCGCCGAGGTCGACTCTGACGACACTGGCGGCAGTGCTGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAACAGCTCACCAAAGTGCACAAGCAGACCTATGTTCCTTATCGAGATAGTAAAATGACCAGAATTCTTCAAGATTCATTAGGTGGCAACTGTAGGACCACTATTGTCATATGCTGCTCTCCATCATCATACAATGAGTCTGAGACAAAGTCAACACTCCTCTTTGGTCAAAGGGCCAAAACAATTAAGAACACAGTCTGTGTCAATGTAGAGTTAACTGCGGAGCAGTGGAAAAAGAAGTATGAAAAAGAAAAGGAAAAAAATAAAACTCTACGGAACACTATTCAGTGGCTGGAAAACGAGCTAAACCGTTGGCGTAACGGGGAGACAGTGCCTATTGATGAGCAGTTTGACAAAGAGAAAGCTAATTTGGAAGCCTTCACAGCGGATAAAGATATTGCTATTACCAGTGATAAACCAGCTGCTGCAGTCGGAATGGCTGGTAGTTTTACCGATGCTGAAAGAAGAAAGTGTGAAGAAGAACTTGCTAAATTGTATAAACAACTTGATGACAAGGATGAAGAGATTAACCAACAAAGCCAATTGGTAGAGAAATTGAAGACACAAATGCTGGATCAGGAAGAGCTTCTGGCATCAACCAGAAGGGATCAAGATAATATGCAAGCTGAACTGAATCGCCTCCAAGCAGAAAATGATGCTTCTAAAGAAGAAGTCAAAGAAGTTTTACAGGCCTTAGAGGAACTGGCTGTTAATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACAAAGGAATATGAATTGCTTAGTGATGAATTGAATCAAAAATCTGCAACTTTAGCAAGTATTGATGCTGAGCTTCAGAAGCTGAAGGAAATGACCAACCACCAGAAGAAACGAGCAGCTGAAATGATGGCATCATTATTAAAAGACCTTGCAGAAATAGGAATTGCTGTGGGGAATAACGATGTGAAGCAACCAGAAGGAACTGGTATGATAGATGAAGAGTTTACTGTTGCAAGACTCTACATTAGCAAAATGAAATCAGAAGTAAAGACCATGGTGAAACGCTGCAAACAGCTAGAAAGCACACAGACTGAGAGCAACAAAAAAATGGAAGAAAATGAGAAAGAGTTAGCAGCATGCCAGCTTCGGATCTCCCAACATGAAGCCAAAATCAAGTCACTGACTGAGTACCTTCAGAATGTAGAACAAAAGAAGAGGCAGCTGGAGGAGTCTGTTGATTCCCTTGGTGAGGAGCTAGTCCAACTCCGAGCACAAGAGAAAGTCCATGAAATGGAAAAAGAGCACTTGAACAAGGTTCAGACTGCAAATGAAGTCAAGCAAGCTGTTGAGCAGCAGATCCAGAGTCACAGAGAAACCCACCAAAAACAAATCAGTAGTTTGCGAGATGAAGTGGAGGCAAAGGAAAAGCTAATCACTGACCTCCAAGACCAAAACCAGAAGATGGTGTTGGAGCAGGAACGGCTAAGGGTGGAGCATGAGAGGCTGAAGGCTACAGACCAAGAGAAGAGCAGGAAGCTGCACGAGCTCACGGTTATGCAAGACAGACGAGAACAAGCAAGACAAGACTTGAAGGGTTTGGAGGAGACCGTGGCAAAAGAACTTCAGACTTTACACAACCTGCGTAAGCTCTTTGTTCAGGACTTGGCTACCAGGGTGAAAAAGAGCGCCGAGGTCGACTCTGACGACACTGGCGGCAGTGCTGCACAGAAGCAGAAAATCTCCTTCCTTGAAAACAACCTTGAACAGCTCACCAAAGTGCACAAGCAG
Peptide sequence:
MTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKTLRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTADKDIAITSDKPAAAVGMAGSFTDAERRKCEEELAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDSLGEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKEKLITDLQDQNQKMVLEQERLRVEHERLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEVDSDDTGGSAAQKQKISFLENNLEQLTKVHKQTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKTLRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTADKDIAITSDKPAAAVGMAGSFTDAERRKCEEELAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDSLGEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKEKLITDLQDQNQKMVLEQERLRVEHERLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEVDSDDTGGSAAQKQKISFLENNLEQLTKVHKQ
IRESfinder result
IRESfinder score
0.411697035
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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