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mmu_circKif21a_3185
Basic information of circular RNA
PanCircBase ID:
mmu_circKif21a_3185
mm39_Chr. Position ID:
chr15|90836913|90878678|-
Gene name:
Kif21a
Transcript:
ENSMUST00000100304.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
24
Exon/intron indexes:
28,27,26,25,24,23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5
Exon/intron offsets:
0,3584,6366,6977,7141,12756,13626,14485,15350,15646,16052,17067,17968,18372,18954,19914,23760,28084,32023,32531,33074,38999,40990,41630
Genomic length:
41765
Exon/intron sizes in nt:
10,82,15,55,61,21,16,60,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr15:90835577-90836913|chr15:90878678-90879686
Splice length:
3185
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGATGCAGTGTCTGAAGCTGGGCGCTCTCTCGCGCACCACGGCCAGCACCCAGATGAACGTACAGAGCTCTCGTTCACACGCCATCTTTACCATTCACGTGTGTCAAACCAGAGTGTGTCCCCAAACAGATGCTGAGAACGCAACTGATAATAAGCTGATCTCCGAATCGTCGCCAATGAATGAGTTTGAGACGCTGACGGCGAAGTTTCACTTTGTTGATCTGGCGGGATCGGAAAGACTGAAGAGAACCGGAGCTACAGGCGAGAGAGCAAAAGAGGGCATTTCCATCAACTGCGGGCTTTTGGCTCTTGGGAATGTAATCAGCGCCTTGGGGGACAAGAGCAAGAGAGCCACCCATGTCCCTTACAGAGACTCTAAGCTGACAAGACTCTTGCAGGATTCCCTTGGGGGTAACAGCCAAACCATCATGATAGCATGCGTCAGCCCTTCAGACAGGGATTTCATGGAGACGTTAAACACTCTGAAGTACGCCAATCGAGCGAGAAATATCAAGAACAAGGTGATGGTCAATCAAGACAGAGCCAGTCAGCAAATCAACGCGCTGCGGAGCGAGATCACGCGCCTTCAGATGGAGCTCATGGAATACAAAACCGGTAAAAGAATAATTGACGAGGAAGGCGTGGAAAGCATCAATGACATGTTTCATGAGAATGCTATGCTGCAGACGGAAAATAATAATCTGCGTGTAAGAATAAAAGCCATGCAGGAGACCGTTGATGCACTGAGGGCCAGAATCACGCAGCTTGTCAGTGAGCAGGCCAACCAAGTTCTTGCCCGAGCAGGTGAAGGGAACGAAGAGATCAGTAATATGATTCATAGTTATATCAAAGAAATTGAAGACCTCAGGGCAAAATTATTAGAAAGTGAAGCAGTGAACGAGAACCTTCGGAAGAACTTGACCAGAGCCACGGCGAGATCTCCTTACTTCAGTGCCTCCTCAGCTTTCTCGCCTACTATACTGTCTTCAGACAAGGAGACTATCGAAATTATAGATCTAGCAAAGAAAGACTTGGAGAAGCTAAAACGGAAAGAGAAGAAGAAGAAGAAAAGGCTACAGAAACTTGAGGAAAGCGGTCGAGAAGAAAGAAGTGTTGCCGGGAAAGACGATAATGCAGACACTGACCAGGAGAAGAAAGAAGAAAAGGGTGTTTCAGAGAAAGAAAACAATGAGCTAGACGTGGAAGAGAATCAAGAAGTGAGTGACCACGAGGATGAGGAAGAGGAGGAAGAGGACGAGGAGGAAGAGGATGACATTGAAGGAGAAGAAAGCTCTGATGAATCAGATTCCGAATCTGATGAAAAAGCTAACTATCAAGCCGACTTAGCAAATATCACCTGCGAGATTGCGATTAAGCAGAAGCTGATCGACGAACTGGAGAACAGCCAGAAACGGCTGCAGACCCTGAAAAAGCAGTACGAGGAGAAGCTGATGATGCTCCAACATAAGATCCGGGACACGCAGCTGGAGAGGGACCAGGTGCTCCAGAACCTAGGCTCGGTGGAGTCATACTCGGAAGAAAAGGCCAAGAAAGTGAAGTGCGAATATGAGAAGAAGCTCCACGCCATGAACAAAGAGCTGCAGCGACTGCAGACGGCCCAAAAGGAGCACGCCAGGCTCCTCAAAAACCAGTCTCAGTATGAAAAGCAGCTCAAGAAACTGCAGCAAGACGTCATGGAAATGAAGAAAACCAAGGTTCGTCTAATGAAGCAGATGAAAGAAGAGCAGGAGAAAGCCCGGCTGACAGAATCTCGAAGGAACCGGGAAATCGCTCAGCTGAAGAAGGATCAGCGCAAGCGAGATCATCAACTTAGACTTCTAGAGGCCCAGAAAAGAAATCAAGAAGTAGTTCTGCGACGCAAAACGGAAGAGGTTACAGCTCTCCGCAGGCAAGTGAGGCCCATGTCTGATAAAGTAGCGGGAAAAGTTACTCGGAAGCTGAGCTCATCCGAAAGCCCCGCTCCGGACACAGGTTCCAGTGCGGCTTCCGGGGAAGCAGACACATCACGGCCAGGCACCCAGCAGAAAATGAGGATCCCCGTGGCAAGAGTCCAGGCATTACCAACACCTACAACAAATGGCACCAGGAAAAAATATCAGAGGAAAGGATTCACTGGCCGGGTGTTCACTTCCAAGACAGCCCGCATGAAGTGGCAGCTACTGGAGCGCCGGGTGACCGACATCATCATGCAGAAAATGACCATCTCCAACATGGAGGCGGACATGAACAGACTCCTCAGGCAACGGGAAGAACTCACAAAAAGGCGAGAGAAACTTTCTAAAAGGAGAGAGAAGATAGTCAAGGAGAGCGGAGAGGGAGATAAAAGTGTGGCTAACATCATCGAGGAGATGGAGTCCCTGACAGCCAACATAGATTACATCAATGATAGCATTGCCGACTGTCAAGCCAACATCATGCAGATGGAGGAGGCAAAGGAAGAAGGGGAGACATTGGATGTCACCGCTGTCATTAATGCCTGTACACTGACAGAAGCTCGGTACCTGCTAGATCACTTCCTGTCAATGGGCATCAATAAGGGTCTGCAGGCTGCCCAGAAAGAGGCTCAAATTAAAGTCCTCGAGGGTCGACTCAAACAGACCGAAATCACCAGTGCAACCCAGAACCAACTCTTATTCCATATGCTGAAGGAGAAGGCAGAGCTAAACCCAGAGCTGGATGCCTTGCTAGGCCACGCACTGCAAGCATTGACTCAGAAGCCCAAACAAAACGAGGAGGACAGCAGCGAGGAAGATGGCCCTTTACACAGCCCGGGGTCGGAGGGAAGCACGTTATCTTCAGACCTTATGAAGCTTTGTGGTGAAGTGAAACCAAAGAACAAGGCTCGAAGGAGAACCACCACTCAGATGGAGTTGCTGTATGCAGATAGCAGTGAAGTAGCCTCAGACACTAGTGCAGGAGATGCCTCCTTGTCTGGCCCTCTGGCACCTGTTGCAGAAGGGCAGGAGATTGGAATGAACACAGAGACAAGTGGTACTTCTGCTAGGGACAAAGAGCTTCTTGCCCCATCTGGCTTACCTTCTAAGATAGGCAGCATTTCCAGACAGTCATCTCTATCAGAAAAAAAAGTGCCAGAGCCTTCCCCTGTAACAAGGAGAAAGGCATATGAGAAGGCAGATAAACCAAAGGCGAAGGAACATAAACA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGAGCCTTCCCCTGTAAC
59.75
CGTGTGAACGAGAGCTCTGT
60.04
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGAGCCTTCCCCTGTAAC
59.75
AGATGGCGTGTGAACGAGAG
60.11
162
PCR template sequence:
AGTCATCTCTATCAGAAAAAAAAGTGCCAGAGCCTTCCCCTGTAACAAGGAGAAAGGCATATGAGAAGGCAGATAAACCAAAGGCGAAGGAACATAAACAATGATGCAGTGTCTGAAGCTGGGCGCTCTCTCGCGCACCACGGCCAGCACCCAGATGAACGTACAGAGCTCTCGTTCACACGCCATCTTTACCATTCACG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
27
Interacting miRNAs (#binding sites)
mmu-miR-207(5), mmu-miR-9-5p(2), mmu-miR-31-5p(2), mmu-miR-136-5p(2), mmu-miR-135a-5p(1), mmu-miR-211-5p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-328-3p(1), mmu-miR-217-5p(1), mmu-miR-125b-5p(1), mmu-miR-25-3p(1), mmu-miR-339-5p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-433-3p(1), mmu-miR-125a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-22-3p(1), mmu-miR-680(1), mmu-miR-29b-2-5p(1), mmu-miR-5101(1), mmu-miR-335-3p(1), mmu-miR-1904(1), mmu-miR-339-3p(1), mmu-miR-181d-5p(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGGAACATAAACAATGATGCAGTGTC
siRNA sets
CATCATTGTTTATGTTCCT,ACTGCATCATTGTTTATGT,GCATCATTGTTTATGTTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF21A_3185_ORF_1
1.1699
0.376705343
0.999999999
3189
ORF sequence:
ATGATGCAGTGTCTGAAGCTGGGCGCTCTCTCGCGCACCACGGCCAGCACCCAGATGAACGTACAGAGCTCTCGTTCACACGCCATCTTTACCATTCACGTGTGTCAAACCAGAGTGTGTCCCCAAACAGATGCTGAGAACGCAACTGATAATAAGCTGATCTCCGAATCGTCGCCAATGAATGAGTTTGAGACGCTGACGGCGAAGTTTCACTTTGTTGATCTGGCGGGATCGGAAAGACTGAAGAGAACCGGAGCTACAGGCGAGAGAGCAAAAGAGGGCATTTCCATCAACTGCGGGCTTTTGGCTCTTGGGAATGTAATCAGCGCCTTGGGGGACAAGAGCAAGAGAGCCACCCATGTCCCTTACAGAGACTCTAAGCTGACAAGACTCTTGCAGGATTCCCTTGGGGGTAACAGCCAAACCATCATGATAGCATGCGTCAGCCCTTCAGACAGGGATTTCATGGAGACGTTAAACACTCTGAAGTACGCCAATCGAGCGAGAAATATCAAGAACAAGGTGATGGTCAATCAAGACAGAGCCAGTCAGCAAATCAACGCGCTGCGGAGCGAGATCACGCGCCTTCAGATGGAGCTCATGGAATACAAAACCGGTAAAAGAATAATTGACGAGGAAGGCGTGGAAAGCATCAATGACATGTTTCATGAGAATGCTATGCTGCAGACGGAAAATAATAATCTGCGTGTAAGAATAAAAGCCATGCAGGAGACCGTTGATGCACTGAGGGCCAGAATCACGCAGCTTGTCAGTGAGCAGGCCAACCAAGTTCTTGCCCGAGCAGGTGAAGGGAACGAAGAGATCAGTAATATGATTCATAGTTATATCAAAGAAATTGAAGACCTCAGGGCAAAATTATTAGAAAGTGAAGCAGTGAACGAGAACCTTCGGAAGAACTTGACCAGAGCCACGGCGAGATCTCCTTACTTCAGTGCCTCCTCAGCTTTCTCGCCTACTATACTGTCTTCAGACAAGGAGACTATCGAAATTATAGATCTAGCAAAGAAAGACTTGGAGAAGCTAAAACGGAAAGAGAAGAAGAAGAAGAAAAGGCTACAGAAACTTGAGGAAAGCGGTCGAGAAGAAAGAAGTGTTGCCGGGAAAGACGATAATGCAGACACTGACCAGGAGAAGAAAGAAGAAAAGGGTGTTTCAGAGAAAGAAAACAATGAGCTAGACGTGGAAGAGAATCAAGAAGTGAGTGACCACGAGGATGAGGAAGAGGAGGAAGAGGACGAGGAGGAAGAGGATGACATTGAAGGAGAAGAAAGCTCTGATGAATCAGATTCCGAATCTGATGAAAAAGCTAACTATCAAGCCGACTTAGCAAATATCACCTGCGAGATTGCGATTAAGCAGAAGCTGATCGACGAACTGGAGAACAGCCAGAAACGGCTGCAGACCCTGAAAAAGCAGTACGAGGAGAAGCTGATGATGCTCCAACATAAGATCCGGGACACGCAGCTGGAGAGGGACCAGGTGCTCCAGAACCTAGGCTCGGTGGAGTCATACTCGGAAGAAAAGGCCAAGAAAGTGAAGTGCGAATATGAGAAGAAGCTCCACGCCATGAACAAAGAGCTGCAGCGACTGCAGACGGCCCAAAAGGAGCACGCCAGGCTCCTCAAAAACCAGTCTCAGTATGAAAAGCAGCTCAAGAAACTGCAGCAAGACGTCATGGAAATGAAGAAAACCAAGGTTCGTCTAATGAAGCAGATGAAAGAAGAGCAGGAGAAAGCCCGGCTGACAGAATCTCGAAGGAACCGGGAAATCGCTCAGCTGAAGAAGGATCAGCGCAAGCGAGATCATCAACTTAGACTTCTAGAGGCCCAGAAAAGAAATCAAGAAGTAGTTCTGCGACGCAAAACGGAAGAGGTTACAGCTCTCCGCAGGCAAGTGAGGCCCATGTCTGATAAAGTAGCGGGAAAAGTTACTCGGAAGCTGAGCTCATCCGAAAGCCCCGCTCCGGACACAGGTTCCAGTGCGGCTTCCGGGGAAGCAGACACATCACGGCCAGGCACCCAGCAGAAAATGAGGATCCCCGTGGCAAGAGTCCAGGCATTACCAACACCTACAACAAATGGCACCAGGAAAAAATATCAGAGGAAAGGATTCACTGGCCGGGTGTTCACTTCCAAGACAGCCCGCATGAAGTGGCAGCTACTGGAGCGCCGGGTGACCGACATCATCATGCAGAAAATGACCATCTCCAACATGGAGGCGGACATGAACAGACTCCTCAGGCAACGGGAAGAACTCACAAAAAGGCGAGAGAAACTTTCTAAAAGGAGAGAGAAGATAGTCAAGGAGAGCGGAGAGGGAGATAAAAGTGTGGCTAACATCATCGAGGAGATGGAGTCCCTGACAGCCAACATAGATTACATCAATGATAGCATTGCCGACTGTCAAGCCAACATCATGCAGATGGAGGAGGCAAAGGAAGAAGGGGAGACATTGGATGTCACCGCTGTCATTAATGCCTGTACACTGACAGAAGCTCGGTACCTGCTAGATCACTTCCTGTCAATGGGCATCAATAAGGGTCTGCAGGCTGCCCAGAAAGAGGCTCAAATTAAAGTCCTCGAGGGTCGACTCAAACAGACCGAAATCACCAGTGCAACCCAGAACCAACTCTTATTCCATATGCTGAAGGAGAAGGCAGAGCTAAACCCAGAGCTGGATGCCTTGCTAGGCCACGCACTGCAAGCATTGACTCAGAAGCCCAAACAAAACGAGGAGGACAGCAGCGAGGAAGATGGCCCTTTACACAGCCCGGGGTCGGAGGGAAGCACGTTATCTTCAGACCTTATGAAGCTTTGTGGTGAAGTGAAACCAAAGAACAAGGCTCGAAGGAGAACCACCACTCAGATGGAGTTGCTGTATGCAGATAGCAGTGAAGTAGCCTCAGACACTAGTGCAGGAGATGCCTCCTTGTCTGGCCCTCTGGCACCTGTTGCAGAAGGGCAGGAGATTGGAATGAACACAGAGACAAGTGGTACTTCTGCTAGGGACAAAGAGCTTCTTGCCCCATCTGGCTTACCTTCTAAGATAGGCAGCATTTCCAGACAGTCATCTCTATCAGAAAAAAAAGTGCCAGAGCCTTCCCCTGTAACAAGGAGAAAGGCATATGAGAAGGCAGATAAACCAAAGGCGAAGGAACATAAACAATGA
Peptide sequence:
MMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQALTQKPKQNEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQ
IRESfinder result
IRESfinder score
0.301513842
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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