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mmu_circKif20a_349
Basic information of circular RNA
PanCircBase ID:
mmu_circKif20a_349
mm39_Chr. Position ID:
chr18|34759768|34760744|+
Gene name:
Kif20a
Transcript:
ENSMUST00000167161.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
3,4,5
Exon/intron offsets:
0,179,837
Genomic length:
976
Exon/intron sizes in nt:
9,01,20,139
Flanking exon/introns:
chr18:34758694-34759768|chr18:34760744-34760837
Splice length:
349
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCTCTACTGGAGGACACCTCAGAGAAGGTGAAAGTTTACCTTCGAATTCGACCATTCTTGACTTCAGAGTTGGACCGACAGGAAGATCAGGGCTGCGTCTGCATTGAGAATACAGAAACCCTTGTGCTGCAGGCACCCAAAGACTCCTTTGCCTTGAAGAGTAATGAGAGGGGAGTTGGTCAGGCCACTCACAAATTTACCTTTTCCCAGATATTTGGGCCAGAAGTGGGACAGGTGGCCTTCTTCAATCTGACCATGAAGGAGATGGTGAAGGATGTGCTCAAAGGGCAGAACTGGCTCATCTACACCTATGGAGTCACCAACTCGGGGAAAACGTACACCATTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGATGTGCTCAAAGGGCA
59.89
TCCTGTCGGTCCAACTCTGA
60.18
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGATGTGCTCAAAGGGCA
59.89
CAGCCCTGATCTTCCTGTCG
60.18
174
PCR template sequence:
CTGACCATGAAGGAGATGGTGAAGGATGTGCTCAAAGGGCAGAACTGGCTCATCTACACCTATGGAGTCACCAACTCGGGGAAAACGTACACCATTCAAGGCTCTACTGGAGGACACCTCAGAGAAGGTGAAAGTTTACCTTCGAATTCGACCATTCTTGACTTCAGAGTTGGACCGACAGGAAGATCAGGGCTGCGTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TACACCATTCAAGGCTCTACTGGAGG
siRNA sets
CCAGTAGAGCCTTGAATGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF20A_349_ORF_1
0.9331
0.325608078
0.70256905
423
ORF sequence:
ATGTGCTCAAAGGGCAGAACTGGCTCATCTACACCTATGGAGTCACCAACTCGGGGAAAACGTACACCATTCAAGGCTCTACTGGAGGACACCTCAGAGAAGGTGAAAGTTTACCTTCGAATTCGACCATTCTTGACTTCAGAGTTGGACCGACAGGAAGATCAGGGCTGCGTCTGCATTGAGAATACAGAAACCCTTGTGCTGCAGGCACCCAAAGACTCCTTTGCCTTGAAGAGTAATGAGAGGGGAGTTGGTCAGGCCACTCACAAATTTACCTTTTCCCAGATATTTGGGCCAGAAGTGGGACAGGTGGCCTTCTTCAATCTGACCATGAAGGAGATGGTGAAGGATGTGCTCAAAGGGCAGAACTGGCTCATCTACACCTATGGAGTCACCAACTCGGGGAAAACGTACACCATTCAA
Peptide sequence:
MCSKGRTGSSTPMESPTRGKRTPFKALLEDTSEKVKVYLRIRPFLTSELDRQEDQGCVCIENTETLVLQAPKDSFALKSNERGVGQATHKFTFSQIFGPEVGQVAFFNLTMKEMVKDVLKGQNWLIYTYGVTNSGKTYTIQ
IRESfinder result
IRESfinder score
0.643695891
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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