top of page
mmu_circKif16b_302
Basic information of circular RNA
PanCircBase ID:
mmu_circKif16b_302
mm39_Chr. Position ID:
chr2|142675996|142689024|-
Gene name:
Kif16b
Transcript:
ENSMUST00000230763.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
12,11,10
Exon/intron offsets:
0,3440,12852
Genomic length:
13028
Exon/intron sizes in nt:
60,66,176
Flanking exon/introns:
chr2:142600829-142675996|chr2:142689024-142689910
Splice length:
302
Other database IDs:
circAtlas: mmu-Kif16b_0038
Human Ortholog IDs:
Mature spliced Sequence:
CCATTTCACCTGCTGATGTCAATTATGGAGAAACCCTAAGTACCCTTCGCTATGCAAATAGAGCCAAAAACATCATCAACAAGCCTACCATTAATGAGGATGCCAACGTCAAACTCATCCGTGAGCTGAGAGCTGAAATAGCCAGGCTGAAAACCCTGCTCGCTCAAGGGAACCAGATTGCCCTCTTGGACTCCCCTACAGCTTTAAGTATGGAGGAAAAACTTCACCAGAATGAAGCAAGAGTCCAAGAATTGACCAAGGAATGGACAAACAAGTGGAATGAAACCCAGAATATTTTGAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAGAATGAAGCAAGAGTCCA
59.63
GCTCTATTTGCATAGCGAAGGG
59.52
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAGAATGAAGCAAGAGTCCA
59.63
GGCTCTATTTGCATAGCGAAGG
59.52
142
PCR template sequence:
TTTAAGTATGGAGGAAAAACTTCACCAGAATGAAGCAAGAGTCCAAGAATTGACCAAGGAATGGACAAACAAGTGGAATGAAACCCAGAATATTTTGAAACCATTTCACCTGCTGATGTCAATTATGGAGAAACCCTAAGTACCCTTCGCTATGCAAATAGAGCCAAAAACATCATCAACAAGCCTACCATTAATGAGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-320-3p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
GAATATTTTGAAACCATTTCACCTGC
siRNA sets
AAATGGTTTCAAAATATTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF16B_302_ORF_1
0.9248
0.093877041
0.099654915
132
ORF sequence:
ATGGAGGAAAAACTTCACCAGAATGAAGCAAGAGTCCAAGAATTGACCAAGGAATGGACAAACAAGTGGAATGAAACCCAGAATATTTTGAAACCATTTCACCTGCTGATGTCAATTATGGAGAAACCCTAA
Peptide sequence:
MEEKLHQNEARVQELTKEWTNKWNETQNILKPFHLLMSIMEKP
IRESfinder result
IRESfinder score
0.477499226
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page