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mmu_circKif16b_213
Basic information of circular RNA
PanCircBase ID:
mmu_circKif16b_213
mm39_Chr. Position ID:
chr2|142514208|142532621|-
Gene name:
Kif16b
Transcript:
ENSMUST00000230763.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
25,24
Exon/intron offsets:
0,18290
Genomic length:
18413
Exon/intron sizes in nt:
90,123
Flanking exon/introns:
chr2:142490431-142514208|chr2:142532621-142544542
Splice length:
213
Other database IDs:
CIRCpedia: MMU_CIRCpedia_9204, circAtlas: mmu-Kif16b_0015
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_368962
Human Ortholog IDs:
Mature spliced Sequence:
CGGATGTATTCTCGCTCTTTGGGAACAAATCGAGACGACCTGAAGGACCCCATTAAGATTAGTATTCCTCGCTATGTCCTGTGTGGCCAAGGGAAGGACGAGCACTTCGAGTTTGAAGTGAAGATTTCTGTCCTAGATGAGACATGGACTGTATTCAGGCGCTACAGTCGTTTTCGAGAAATGCATAAAACATTGAAGCTAAAGTATGCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGGCGCTACAGTCGTTTT
59.97
CCACACAGGACATAGCGAGG
60.18
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCAGGCGCTACAGTCGTTT
59.97
CCACACAGGACATAGCGAGG
60.18
146
PCR template sequence:
TGAAGTGAAGATTTCTGTCCTAGATGAGACATGGACTGTATTCAGGCGCTACAGTCGTTTTCGAGAAATGCATAAAACATTGAAGCTAAAGTATGCAGAGCGGATGTATTCTCGCTCTTTGGGAACAAATCGAGACGACCTGAAGGACCCCATTAAGATTAGTATTCCTCGCTATGTCCTGTGTGGCCAAGGGAAGGACG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AAAGTATGCAGAGCGGATGTATTCTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF16B_213_ORF_1
0.853
0.292813621
0.641727914
423
ORF sequence:
ATGTATTCTCGCTCTTTGGGAACAAATCGAGACGACCTGAAGGACCCCATTAAGATTAGTATTCCTCGCTATGTCCTGTGTGGCCAAGGGAAGGACGAGCACTTCGAGTTTGAAGTGAAGATTTCTGTCCTAGATGAGACATGGACTGTATTCAGGCGCTACAGTCGTTTTCGAGAAATGCATAAAACATTGAAGCTAAAGTATGCAGAGCGGATGTATTCTCGCTCTTTGGGAACAAATCGAGACGACCTGAAGGACCCCATTAAGATTAGTATTCCTCGCTATGTCCTGTGTGGCCAAGGGAAGGACGAGCACTTCGAGTTTGAAGTGAAGATTTCTGTCCTAGATGAGACATGGACTGTATTCAGGCGCTACAGTCGTTTTCGAGAAATGCATAAAACATTGAAGCTAAAGTATGCAGAG
Peptide sequence:
MYSRSLGTNRDDLKDPIKISIPRYVLCGQGKDEHFEFEVKISVLDETWTVFRRYSRFREMHKTLKLKYAERMYSRSLGTNRDDLKDPIKISIPRYVLCGQGKDEHFEFEVKISVLDETWTVFRRYSRFREMHKTLKLKYAE
IRESfinder result
IRESfinder score
0.478880167
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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