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mmu_circKif13b_907
Basic information of circular RNA
PanCircBase ID:
mmu_circKif13b_907
mm39_Chr. Position ID:
chr14|64959755|64976090|+
Gene name:
Kif13b
Transcript:
ENSMUST00000100473.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
7,8,9,10,11,12,13
Exon/intron offsets:
0,2393,4767,5906,13841,15188,16200
Genomic length:
16335
Exon/intron sizes in nt:
8,81,35,11,31,12,21,31,00,000
Flanking exon/introns:
chr14:64955103-64959755|chr14:64976090-64979755
Splice length:
907
Other database IDs:
CIRCpedia: MMU_CIRCpedia_227729, circAtlas: mmu-Kif13b_0013
Human Ortholog IDs:
Mature spliced Sequence:
AAGCCGTCAGACGTTGAAAGTGAGAGAGCACAGTGTGTTGGGACCCTACGTGGATGGACTTTCTAAACTGGCTGTCACAAGCTACAAGGATATTGAGTCTCTGATGTCTGAGGGCAACAAGTCTCGGACAGTTGCTGCAACCAACATGAACGAGGAAAGTAGCCGTTCCCATGCAGTCTTCAAAATCACCCTGACACATACTCTGTATGATGTGAAGTCTGGGACATCTGGAGAGAAAGTGGGCAAGCTGAGCTTAGTGGATTTAGCTGGCAGTGAACGAGCCACGAAAACCGGAGCTGCGGGCGACAGGCTGAAGGAGGGCAGCAACATCAACAAGTCCCTCACAACCCTGGGTCTGGTTATCTCTGCCCTAGCTGATCAGGGTGCTGGCAAAAACAAAAATAAATTTGTTCCCTATCGGGACTCAGTTCTCACATGGCTGCTCAAGGACAGTCTGGGAGGTAATAGCAAGACTGCCATGGTGGCCACTGTGAGCCCCGCTGCAGATAACTATGACGAAACCCTCTCAACCCTGAGGTACGCAGATCGAGCAAAGCACATCGTCAACCATGCTGTGGTGAATGAGGATCCGAATGCTCGGATTATCCGAGATCTGCGGGAGGAGGTGGAGAAGCTCCGGGAGCAGCTGACCAAGGCAGAGGCAATGAAATCTCCAGAGCTGAAAGATCGTCTGGAAGAATCGGAGAAACTCATCCAGGAAATGACTGTGACCTGGGAGGAGAAACTAAGGAAAACTGAGGAGATTGCGCAGGAGCGGCAAAAGCAGCTTGAGAGCCTTGGCATATCTCTTCAGACTTCTGGAATCAAGGTTGGGGATGACAAGTGCTTCCTGGTTAACCTGAATGCTGACCCAGCTCTGAATGAACTCCTGGTGTACTACTTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGGATGACAAGTGCTTCC
59.96
TGTAGCTTGTGACAGCCAGT
59.24
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTTGGGGATGACAAGTGC
60.25
AGTCCATCCACGTAGGGTCC
60.69
140
PCR template sequence:
TCTTCAGACTTCTGGAATCAAGGTTGGGGATGACAAGTGCTTCCTGGTTAACCTGAATGCTGACCCAGCTCTGAATGAACTCCTGGTGTACTACTTAAAGAAGCCGTCAGACGTTGAAAGTGAGAGAGCACAGTGTGTTGGGACCCTACGTGGATGGACTTTCTAAACTGGCTGTCACAAGCTACAAGGATATTGAGTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-296-3p(2), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-146a-5p(1), mmu-miR-217-5p(1), mmu-miR-382-3p(1), mmu-miR-541-5p(1), mmu-miR-207(1), mmu-miR-1199-5p(1), mmu-miR-574-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTACTACTTAAAGAAGCCGTCAGACG
siRNA sets
TCTGACGGCTTCTTTAAGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF13B_907_ORF_1
1.1346
0.391128213
0.991002786
870
ORF sequence:
ATGTCTGAGGGCAACAAGTCTCGGACAGTTGCTGCAACCAACATGAACGAGGAAAGTAGCCGTTCCCATGCAGTCTTCAAAATCACCCTGACACATACTCTGTATGATGTGAAGTCTGGGACATCTGGAGAGAAAGTGGGCAAGCTGAGCTTAGTGGATTTAGCTGGCAGTGAACGAGCCACGAAAACCGGAGCTGCGGGCGACAGGCTGAAGGAGGGCAGCAACATCAACAAGTCCCTCACAACCCTGGGTCTGGTTATCTCTGCCCTAGCTGATCAGGGTGCTGGCAAAAACAAAAATAAATTTGTTCCCTATCGGGACTCAGTTCTCACATGGCTGCTCAAGGACAGTCTGGGAGGTAATAGCAAGACTGCCATGGTGGCCACTGTGAGCCCCGCTGCAGATAACTATGACGAAACCCTCTCAACCCTGAGGTACGCAGATCGAGCAAAGCACATCGTCAACCATGCTGTGGTGAATGAGGATCCGAATGCTCGGATTATCCGAGATCTGCGGGAGGAGGTGGAGAAGCTCCGGGAGCAGCTGACCAAGGCAGAGGCAATGAAATCTCCAGAGCTGAAAGATCGTCTGGAAGAATCGGAGAAACTCATCCAGGAAATGACTGTGACCTGGGAGGAGAAACTAAGGAAAACTGAGGAGATTGCGCAGGAGCGGCAAAAGCAGCTTGAGAGCCTTGGCATATCTCTTCAGACTTCTGGAATCAAGGTTGGGGATGACAAGTGCTTCCTGGTTAACCTGAATGCTGACCCAGCTCTGAATGAACTCCTGGTGTACTACTTAAAGAAGCCGTCAGACGTTGAAAGTGAGAGAGCACAGTGTGTTGGGACCCTACGTGGATGGACTTTCTAA
Peptide sequence:
MSEGNKSRTVAATNMNEESSRSHAVFKITLTHTLYDVKSGTSGEKVGKLSLVDLAGSERATKTGAAGDRLKEGSNINKSLTTLGLVISALADQGAGKNKNKFVPYRDSVLTWLLKDSLGGNSKTAMVATVSPAADNYDETLSTLRYADRAKHIVNHAVVNEDPNARIIRDLREEVEKLREQLTKAEAMKSPELKDRLEESEKLIQEMTVTWEEKLRKTEEIAQERQKQLESLGISLQTSGIKVGDDKCFLVNLNADPALNELLVYYLKKPSDVESERAQCVGTLRGWTF
IRESfinder result
IRESfinder score
0.378903744
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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