top of page
mmu_circKif13b_695
Basic information of circular RNA
PanCircBase ID:
mmu_circKif13b_695
mm39_Chr. Position ID:
chr14|64995999|65005188|+
Gene name:
Kif13b
Transcript:
ENSMUST00000100473.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
23,24,25,26,27,28
Exon/intron offsets:
0,2118,6452,7160,8803,9062
Genomic length:
9189
Exon/intron sizes in nt:
15,81,33,14,73,01,00,100
Flanking exon/introns:
chr14:64995033-64995999|chr14:65005188-65005868
Splice length:
695
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAGTTCAGTGTTAACATCACAGAAGACTTCATTGAATATCTCTCAGAAGGGGCCCTAGCCATTGAAGTATATGGACATAAAATGAATGATCCCCGGAAAAACCCAGCCCTGTGGGACTTGGGGATCATCCAAGCAAAAACAAGGAGTCTTCGGGACAGGTGGAGTGAAGTCACCAGAAAGTTGGAATTCTGGGTTCAGATCTTGGAACAGAATGAGAATGGTGATTACTGTCCGGTGGAAGTAATTGCTGCAAAAGATGTCCCCACAGGAGGGATCTTCCAGCTCCGACAGGGGCAGTCTCGGCGAGTTCAAGTTGAAGTGAAGTCTGTGCAGGAGTCAGGAACTTTACCACTGATGGAAGAGTGCATATTGTCAGTTGGCATTGGGTGTGTGAAAGTTAGGCCACTCCGGTCCCCTAAGATCCACGAGAATGTCCATGAGGAAGAGGAGGACATGGACAGCTACCAGGATAGGGATTTAGAGCGGCTTCGTAGAAAATGGCTCAATGCATTAACCAAACGTCAAGAATACTTGGATCAACAACTGCAGAAGCTTGTCAGTAAACATGACAAAACTGAGGATGATGCTGACCGAGAGGCACAGCTCCTGGAGATGCGGCTGACGCTCACAGAGGAAAGAAATGCAGTGATGGTGCCTTCTGCTGGGAGTGGCATTCCAGGGGCCCCTGCAGAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGCACAGCTCCTGGAGAT
60.31
CTAGGGCCCCTTCTGAGAGA
59.74
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGCACAGCTCCTGGAGAT
60.31
AGGGCCCCTTCTGAGAGATA
59.06
156
PCR template sequence:
AGGCACAGCTCCTGGAGATGCGGCTGACGCTCACAGAGGAAAGAAATGCAGTGATGGTGCCTTCTGCTGGGAGTGGCATTCCAGGGGCCCCTGCAGAATGGAGTTCAGTGTTAACATCACAGAAGACTTCATTGAATATCTCTCAGAAGGGGCCCTAGCCATTGAAGTATATGGACATAAAATGAATGATCCCCGGAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-377-3p(1), mmu-miR-188-3p(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCCCTGCAGAATGGAGTTCAGTGTTA
siRNA sets
TAACACTGAACTCCATTCT,AACACTGAACTCCATTCTG,ACACTGAACTCCATTCTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKIF13B_695_ORF_1
1.0835
0.279049352
0.947984658
696
ORF sequence:
ATGGAGTTCAGTGTTAACATCACAGAAGACTTCATTGAATATCTCTCAGAAGGGGCCCTAGCCATTGAAGTATATGGACATAAAATGAATGATCCCCGGAAAAACCCAGCCCTGTGGGACTTGGGGATCATCCAAGCAAAAACAAGGAGTCTTCGGGACAGGTGGAGTGAAGTCACCAGAAAGTTGGAATTCTGGGTTCAGATCTTGGAACAGAATGAGAATGGTGATTACTGTCCGGTGGAAGTAATTGCTGCAAAAGATGTCCCCACAGGAGGGATCTTCCAGCTCCGACAGGGGCAGTCTCGGCGAGTTCAAGTTGAAGTGAAGTCTGTGCAGGAGTCAGGAACTTTACCACTGATGGAAGAGTGCATATTGTCAGTTGGCATTGGGTGTGTGAAAGTTAGGCCACTCCGGTCCCCTAAGATCCACGAGAATGTCCATGAGGAAGAGGAGGACATGGACAGCTACCAGGATAGGGATTTAGAGCGGCTTCGTAGAAAATGGCTCAATGCATTAACCAAACGTCAAGAATACTTGGATCAACAACTGCAGAAGCTTGTCAGTAAACATGACAAAACTGAGGATGATGCTGACCGAGAGGCACAGCTCCTGGAGATGCGGCTGACGCTCACAGAGGAAAGAAATGCAGTGATGGTGCCTTCTGCTGGGAGTGGCATTCCAGGGGCCCCTGCAGAA
Peptide sequence:
MEFSVNITEDFIEYLSEGALAIEVYGHKMNDPRKNPALWDLGIIQAKTRSLRDRWSEVTRKLEFWVQILEQNENGDYCPVEVIAAKDVPTGGIFQLRQGQSRRVQVEVKSVQESGTLPLMEECILSVGIGCVKVRPLRSPKIHENVHEEEEDMDSYQDRDLERLRRKWLNALTKRQEYLDQQLQKLVSKHDKTEDDADREAQLLEMRLTLTEERNAVMVPSAGSGIPGAPAE
IRESfinder result
IRESfinder score
0.3997176
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page