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mmu_circKat2b_958
Basic information of circular RNA
PanCircBase ID:
mmu_circKat2b_958
mm39_Chr. Position ID:
chr17|53948220|53967200|+
Gene name:
Kat2b
Transcript:
ENSMUST00000000724.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
7,8,9,10,11,12,13
Exon/intron offsets:
0,3421,7554,11829,13246,18125,18836
Genomic length:
18980
Exon/intron sizes in nt:
10,71,23,13,72,09,12,70,00,000
Flanking exon/introns:
chr17:53945571-53948220|chr17:53967200-53970562
Splice length:
958
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTCTGTCCATGTTGGAAGAAGAAGTGTATAGTCAAAATTCTCCTATCTGGGATCAGGATTTTCTCTCAGCCTCTTCCAGAACCAGCCCGCTAGGAATCCAAACAGTAATCAGTCCTCCTGTTACTGGGACAGCATTGTTCAGTTCAAATTCCACTTCCCATGAGCAGATCAATGGAGGGAGAACAAGTCCTGGATGCAGAGGCTCTTCTGGGCTTGAAGCAAACCCGGGAGAAAAGAGGAAAATGAACAACTCTCATGCTCCCGAGGAGGCCAAGAGATCTCGAGTGATGGGGGATATTCCCGTGGAATTGATCAATGAGGTCATGTCTACCATCACAGACCCTGCAGGGATGCTTGGACCAGAGACCAATTTTCTGTCAGCCCATTCGGCCAGAGATGAGGCGGCACGGCTGGAAGAACGCAGGGGTGTCATTGAATTCCACGTGGTGGGCAACTCCCTGAACCAGAAACCAAACAAGAAGATCCTGATGTGGCTCGTGGGCCTCCAGAATGTGTTTTCCCACCAGCTGCCCAGAATGCCCAAAGAGTACATCACACGGCTCGTCTTTGACCCGAAACACAAAACCCTTGCTTTAATTAAAGATGGCCGTGTCATTGGTGGTATCTGTTTCCGGATGTTTCCATCCCAGGGATTCACAGAGATTGTTTTCTGTGCAGTAACCTCAAATGAACAAGTCAAGGGCTATGGAACCCACCTGATGAACCATCTCAAAGAATACCACATAAAGCACGAGATCCTCAACTTCCTCACATATGCAGATGAGTATGCCATCGGCTATTTCAAGAAGCAGGGTTTCTCCAAAGAAATCAAAATACCTAAAACCAAATATGTTGGCTACATCAAGGATTATGAAGGGGCCACTTTGATGGGATGTGAGCTGAACCCTCAGATCCCATACACAGAGTTCTCTGTCATCATTAAAAAGCAGAAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGATGTGAGCTGAACCCTC
60.11
GATTCCTAGCGGGCTGGTTC
60.53
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTGAACCCTCAGATCCCA
60.25
GATTCCTAGCGGGCTGGTTC
60.53
159
PCR template sequence:
CTACATCAAGGATTATGAAGGGGCCACTTTGATGGGATGTGAGCTGAACCCTCAGATCCCATACACAGAGTTCTCTGTCATCATTAAAAAGCAGAAGGAGGTTTCTGTCCATGTTGGAAGAAGAAGTGTATAGTCAAAATTCTCCTATCTGGGATCAGGATTTTCTCTCAGCCTCTTCCAGAACCAGCCCGCTAGGAATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-324-5p(1), mmu-miR-136-5p(1), mmu-miR-494-3p(1), mmu-miR-425-5p(1), mmu-miR-802-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
AAAGCAGAAGGAGGTTTCTGTCCATG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKAT2B_958_ORF_1
1.054
0.213961463
0.990302952
981
ORF sequence:
ATGTTGGAAGAAGAAGTGTATAGTCAAAATTCTCCTATCTGGGATCAGGATTTTCTCTCAGCCTCTTCCAGAACCAGCCCGCTAGGAATCCAAACAGTAATCAGTCCTCCTGTTACTGGGACAGCATTGTTCAGTTCAAATTCCACTTCCCATGAGCAGATCAATGGAGGGAGAACAAGTCCTGGATGCAGAGGCTCTTCTGGGCTTGAAGCAAACCCGGGAGAAAAGAGGAAAATGAACAACTCTCATGCTCCCGAGGAGGCCAAGAGATCTCGAGTGATGGGGGATATTCCCGTGGAATTGATCAATGAGGTCATGTCTACCATCACAGACCCTGCAGGGATGCTTGGACCAGAGACCAATTTTCTGTCAGCCCATTCGGCCAGAGATGAGGCGGCACGGCTGGAAGAACGCAGGGGTGTCATTGAATTCCACGTGGTGGGCAACTCCCTGAACCAGAAACCAAACAAGAAGATCCTGATGTGGCTCGTGGGCCTCCAGAATGTGTTTTCCCACCAGCTGCCCAGAATGCCCAAAGAGTACATCACACGGCTCGTCTTTGACCCGAAACACAAAACCCTTGCTTTAATTAAAGATGGCCGTGTCATTGGTGGTATCTGTTTCCGGATGTTTCCATCCCAGGGATTCACAGAGATTGTTTTCTGTGCAGTAACCTCAAATGAACAAGTCAAGGGCTATGGAACCCACCTGATGAACCATCTCAAAGAATACCACATAAAGCACGAGATCCTCAACTTCCTCACATATGCAGATGAGTATGCCATCGGCTATTTCAAGAAGCAGGGTTTCTCCAAAGAAATCAAAATACCTAAAACCAAATATGTTGGCTACATCAAGGATTATGAAGGGGCCACTTTGATGGGATGTGAGCTGAACCCTCAGATCCCATACACAGAGTTCTCTGTCATCATTAAAAAGCAGAAGGAGGTTTCTGTCCATGTTGGAAGAAGAAGTGTATAG
Peptide sequence:
MLEEEVYSQNSPIWDQDFLSASSRTSPLGIQTVISPPVTGTALFSSNSTSHEQINGGRTSPGCRGSSGLEANPGEKRKMNNSHAPEEAKRSRVMGDIPVELINEVMSTITDPAGMLGPETNFLSAHSARDEAARLEERRGVIEFHVVGNSLNQKPNKKILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICFRMFPSQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHEILNFLTYADEYAIGYFKKQGFSKEIKIPKTKYVGYIKDYEGATLMGCELNPQIPYTEFSVIIKKQKEVSVHVGRRSV
IRESfinder result
IRESfinder score
0.594021453
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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