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mmu_circKank1_2986
Basic information of circular RNA
PanCircBase ID:
mmu_circKank1_2986
mm39_Chr. Position ID:
chr19|25386449|25400391|+
Gene name:
Kank1
Transcript:
ENSMUST00000049400.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,6,7
Exon/intron offsets:
0,13058,13833
Genomic length:
13942
Exon/intron sizes in nt:
2,67,91,98,109
Flanking exon/introns:
chr19:25355894-25386449|chr19:25400391-25401501
Splice length:
2986
Other database IDs:
CIRCpedia: MMU_CIRCpedia_14514
Human Ortholog IDs:
Mature spliced Sequence:
AAAAAGCAGACGGAGTTCTGAATGGAGACCATAACAAGGAAAAGAAAGACCCGTATTTTGTGGAAACCCCTTACGGTTTTCAGCTAGACTTAGATTTCGTCAAATACGTGGATGACATACAAAAGGGAAATACTATCAAGAAACTGAACATCCAGAAGAGGCGAAAGCCATCTGTGCCATGTCCAGAAGTCAGGGCCATACCTGGTCATCAAGGTGTGTGGACTTCCACTGAGTCTCTGTCATCCTCCAACAGTGATGACAGCAAGCAGTGTCCCAGCTTCCTGCTAGCCAGAAGCCACGTTACTTCAACCCCAATCCCACGGCCACCTGCCCCGCTAGAGACCTCACCCACTTTTGCTGTGTCAGAAAACCGACAGCTGCTGCCCCCTCCCTCACCACAACTCCCCAGGCACAACCTCCATGTCACCAAGACGTTGATGGAGACCCGGAGAAGACTGGAACAGGAGCGAGTCACCATGCAGATGGCACCGGGTGACTTCAGGAGGCCCAGGCTGGCCAGTTTTGGAGGCATGGGCTCCACAAGCTCCCTCCCGTCCTTTGTGGGGTCTGCTAACCACAGTTCTGCAATACACCAGCTTCAGAATGGCTACCAAGGCAATGGGGATTATAACAGCTACGTCCCAGCAGCTCCTACCACGTCTTCCATGGGAAGCTCCGTCCGGCACAGCCCATTGAGTTCAGGGATCTCCACCCCAGTGACCAACGTGAGCCCCATGCACCTGCAGCACATCCGAGAGCAGATGGCCATCGCCTTAAAACGCCTGAAGGAGCTTGAGGAGCAGGTGAGGACCATCCCTGTGCTCCAGGTCAAGATCTCCGTCTTGCAAGAAGAGAAAAGGCAGTTGGCCTCGCAGCTGAAAAGCCAGAGGGCCTCGTCCCAGAACGAAGCGTGCGGTGTGAGGAAACGCTCCTACAGTGCGGGCAACGCCTCCCAGCTGGAACTGCTTGCCCGAGCCCGAAGAGGCGGCGGGGAATTGTACATCGACTACGAGGAGGAAGAGATGGAGAGCGTAGAGCAGAGCACGCAGCGGATCCAGGAGTTCCGACAGCTCACGGCCGACATGCAGGCGCTGGAGCGGAAGATTCAGGATAGCAGCTGCGAGGTGGCATCAGAGCTCAGGGAGAATGGGCAGTGCCCGTCTCGGGAGTGCAAGTCTGTGGCCGTGGGTAGTGATGAGAACATGAACGATGTCGTTGTATACCACAGAGACTTAAGGCCCTGCAAGGATACGGCTGTGGGGACGGTCACCGAGACGAGGAATGTTGGCATCAGCGTGACAGAAGCTATGCTTGGGGTTATCACTGAAGCTGACAAAGAGATTGAGCTGCAGCAGCAGACCATAGAGGCCTTGAAGGAGAAGATTTACCGCCTAGAAGTACAGCTTAAAGAAACCACCCATGACCGCGAGATGACTAAGCTCAAGCAAGAACTACAGGCTGCCGGATCCAGGAAGAAAGTTGACAAGGCCACAATGGCCCAGCCACTTGTCTTCAGCAAGTTGGTGGAGGCACTGGTGCCAACCAGAGATCAAATGGTTGGCAGTCATGTGGACACAAGGGAGTCATGTGTTGGGACCTCTGTGCAAACTAGTAGCGTAGGCACCTCCTGCCATCCTGACTGCAAGAACCAAGTCGTGGGATCCGAGCTGCCCATGAATTGGTGGGTCGTGAAGGAGAGGGTGGCAATGCATGACCAATGCGTAGGGAGGTCTGTTGAGACTTGTGACCGGAGTGTGGGTGTGGAAGTCAGTGTCTGTGAAACAGGCAGCAACACAGAGGCTTCTGGGAGCGACCTGACACTCCTTAAGACAAACTTGAACCTCAAAGACGTGCGGTCCATCGGCTGTGGAGATTGTTCCGTGGATGTGATTGTCTGCTTCCCCAAGGAGTGCACCTCCCGAAGCATGAACACGGAGGCTGTAGGCCAGGAGGAAGCAGCTGTCATGGCGGTGCCCCATACCACAGACCAGCACACCAGCACGAATCTGGAGCGGGTGGACCAGTGCACTAACACAGAGGCAGCCACCCTGGTGGAGTCCTGCACCAACACTCTCCTCAGCACTATGGACAAGCAGACCAGCACCCAGACTGTGGAGATGCGAACGGTGGCCATCGGAGAAGGCCGCGTCAGAGACATCAACCCCTCCACTAAGACTCGGTCCGTTGGTGTCGGGACAGTGCTGTCTGGCAATTCTGAGTTTGACAGGCCATGTGCTGTGAAGACCAAAGAGTCGGGTGTGGGACAGATAAATATTCAGGACAACTATCTAGTTGGTCTCAAAATGCGGACCATAGCGTGCGGGCCTCCACAGTTGACTGTGGGGCTGATGGGCAGCAGGAGGAGCGTGGGTGTTGGGAACGAGCCCGTAGGAGAGCTCCCGGAGGAGTCTCCTCAGCCTCGGGTGGCGTCTGGAATGGTGACTGGCTTGGATCACTACATTGAGCGCGTGCAGAGGCTGCTGGCAGAGCAACAGACGTTGCTGGCTGAGAACTACAGTGAGCTGGCGGAGGCTTTTGGGGAGCCTCATTCACAGATTGGCTCCCTCAACTCGCAGCTCATCAGCACCCTGTCGTCCATCAATTCCGTCATGAAGTCTGCAAGCACCGAGGAGCTCAGGAACTCTGACTTCCAGAAAGCCAGTCTGGGTAAAACTACAGGAAATCACTTGGAATATACCTGCAAGTGTGGAGGCCTTCGGTCAGGAGGACTGTTAAATGTACAACCATCCCAGCCTGAGGTGGAGGCGGAGACTGCAGAAGGCAAGCACAGCCGGGGCCATGAGCAGTTCCCCATGCAAGGAAGTACCCTGCCCCCGGTGAACCTGACAGATGACCAGATAGCCACTGGCCTCTACGTATGTCCAAATAATGAAAACACACTGAAGTCCATCATGAAGAAAAGTGATGGGAATAAAGATTCAAATGGAGCCAAAAAGAATCTTCAGTTCATTGGCATTAACGGCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACACACTGAAGTCCATCATGAAGA
59.96
GGGGTTTCCACAAAATACGGG
59.45
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACACACTGAAGTCCATCATGA
56.94
GGGGTTTCCACAAAATACGGG
59.45
161
PCR template sequence:
ATAATGAAAACACACTGAAGTCCATCATGAAGAAAAGTGATGGGAATAAAGATTCAAATGGAGCCAAAAAGAATCTTCAGTTCATTGGCATTAACGGCGGAAAAAGCAGACGGAGTTCTGAATGGAGACCATAACAAGGAAAAGAAAGACCCGTATTTTGTGGAAACCCCTTACGGTTTTCAGCTAGACTTAGATTTCGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
22
Interacting miRNAs (#binding sites)
mmu-miR-207(3), mmu-miR-136-5p(2), mmu-miR-335-3p(2), mmu-miR-670-5p(2), mmu-miR-9-5p(1), mmu-miR-7b-5p(1), mmu-miR-135a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-132-3p(1), mmu-miR-138-5p(1), mmu-miR-127-3p(1), mmu-miR-433-3p(1), mmu-miR-96-5p(1), mmu-miR-212-3p(1), mmu-miR-183-5p(1), mmu-miR-362-5p(1), mmu-miR-297a-5p(1), mmu-miR-224-5p(1), mmu-miR-673-5p(1), mmu-miR-669d-5p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCATTAACGGCGGAAAAAGCAGACGG
siRNA sets
CCGTCTGCTTTTTCCGCCG, CTTTTTCCGCCGTTAATGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKANK1_2986_ORF_1
1.1299
0.357096326
0.999999998
3045
ORF sequence:
ATGGGAATAAAGATTCAAATGGAGCCAAAAAGAATCTTCAGTTCATTGGCATTAACGGCGGAAAAAGCAGACGGAGTTCTGAATGGAGACCATAACAAGGAAAAGAAAGACCCGTATTTTGTGGAAACCCCTTACGGTTTTCAGCTAGACTTAGATTTCGTCAAATACGTGGATGACATACAAAAGGGAAATACTATCAAGAAACTGAACATCCAGAAGAGGCGAAAGCCATCTGTGCCATGTCCAGAAGTCAGGGCCATACCTGGTCATCAAGGTGTGTGGACTTCCACTGAGTCTCTGTCATCCTCCAACAGTGATGACAGCAAGCAGTGTCCCAGCTTCCTGCTAGCCAGAAGCCACGTTACTTCAACCCCAATCCCACGGCCACCTGCCCCGCTAGAGACCTCACCCACTTTTGCTGTGTCAGAAAACCGACAGCTGCTGCCCCCTCCCTCACCACAACTCCCCAGGCACAACCTCCATGTCACCAAGACGTTGATGGAGACCCGGAGAAGACTGGAACAGGAGCGAGTCACCATGCAGATGGCACCGGGTGACTTCAGGAGGCCCAGGCTGGCCAGTTTTGGAGGCATGGGCTCCACAAGCTCCCTCCCGTCCTTTGTGGGGTCTGCTAACCACAGTTCTGCAATACACCAGCTTCAGAATGGCTACCAAGGCAATGGGGATTATAACAGCTACGTCCCAGCAGCTCCTACCACGTCTTCCATGGGAAGCTCCGTCCGGCACAGCCCATTGAGTTCAGGGATCTCCACCCCAGTGACCAACGTGAGCCCCATGCACCTGCAGCACATCCGAGAGCAGATGGCCATCGCCTTAAAACGCCTGAAGGAGCTTGAGGAGCAGGTGAGGACCATCCCTGTGCTCCAGGTCAAGATCTCCGTCTTGCAAGAAGAGAAAAGGCAGTTGGCCTCGCAGCTGAAAAGCCAGAGGGCCTCGTCCCAGAACGAAGCGTGCGGTGTGAGGAAACGCTCCTACAGTGCGGGCAACGCCTCCCAGCTGGAACTGCTTGCCCGAGCCCGAAGAGGCGGCGGGGAATTGTACATCGACTACGAGGAGGAAGAGATGGAGAGCGTAGAGCAGAGCACGCAGCGGATCCAGGAGTTCCGACAGCTCACGGCCGACATGCAGGCGCTGGAGCGGAAGATTCAGGATAGCAGCTGCGAGGTGGCATCAGAGCTCAGGGAGAATGGGCAGTGCCCGTCTCGGGAGTGCAAGTCTGTGGCCGTGGGTAGTGATGAGAACATGAACGATGTCGTTGTATACCACAGAGACTTAAGGCCCTGCAAGGATACGGCTGTGGGGACGGTCACCGAGACGAGGAATGTTGGCATCAGCGTGACAGAAGCTATGCTTGGGGTTATCACTGAAGCTGACAAAGAGATTGAGCTGCAGCAGCAGACCATAGAGGCCTTGAAGGAGAAGATTTACCGCCTAGAAGTACAGCTTAAAGAAACCACCCATGACCGCGAGATGACTAAGCTCAAGCAAGAACTACAGGCTGCCGGATCCAGGAAGAAAGTTGACAAGGCCACAATGGCCCAGCCACTTGTCTTCAGCAAGTTGGTGGAGGCACTGGTGCCAACCAGAGATCAAATGGTTGGCAGTCATGTGGACACAAGGGAGTCATGTGTTGGGACCTCTGTGCAAACTAGTAGCGTAGGCACCTCCTGCCATCCTGACTGCAAGAACCAAGTCGTGGGATCCGAGCTGCCCATGAATTGGTGGGTCGTGAAGGAGAGGGTGGCAATGCATGACCAATGCGTAGGGAGGTCTGTTGAGACTTGTGACCGGAGTGTGGGTGTGGAAGTCAGTGTCTGTGAAACAGGCAGCAACACAGAGGCTTCTGGGAGCGACCTGACACTCCTTAAGACAAACTTGAACCTCAAAGACGTGCGGTCCATCGGCTGTGGAGATTGTTCCGTGGATGTGATTGTCTGCTTCCCCAAGGAGTGCACCTCCCGAAGCATGAACACGGAGGCTGTAGGCCAGGAGGAAGCAGCTGTCATGGCGGTGCCCCATACCACAGACCAGCACACCAGCACGAATCTGGAGCGGGTGGACCAGTGCACTAACACAGAGGCAGCCACCCTGGTGGAGTCCTGCACCAACACTCTCCTCAGCACTATGGACAAGCAGACCAGCACCCAGACTGTGGAGATGCGAACGGTGGCCATCGGAGAAGGCCGCGTCAGAGACATCAACCCCTCCACTAAGACTCGGTCCGTTGGTGTCGGGACAGTGCTGTCTGGCAATTCTGAGTTTGACAGGCCATGTGCTGTGAAGACCAAAGAGTCGGGTGTGGGACAGATAAATATTCAGGACAACTATCTAGTTGGTCTCAAAATGCGGACCATAGCGTGCGGGCCTCCACAGTTGACTGTGGGGCTGATGGGCAGCAGGAGGAGCGTGGGTGTTGGGAACGAGCCCGTAGGAGAGCTCCCGGAGGAGTCTCCTCAGCCTCGGGTGGCGTCTGGAATGGTGACTGGCTTGGATCACTACATTGAGCGCGTGCAGAGGCTGCTGGCAGAGCAACAGACGTTGCTGGCTGAGAACTACAGTGAGCTGGCGGAGGCTTTTGGGGAGCCTCATTCACAGATTGGCTCCCTCAACTCGCAGCTCATCAGCACCCTGTCGTCCATCAATTCCGTCATGAAGTCTGCAAGCACCGAGGAGCTCAGGAACTCTGACTTCCAGAAAGCCAGTCTGGGTAAAACTACAGGAAATCACTTGGAATATACCTGCAAGTGTGGAGGCCTTCGGTCAGGAGGACTGTTAAATGTACAACCATCCCAGCCTGAGGTGGAGGCGGAGACTGCAGAAGGCAAGCACAGCCGGGGCCATGAGCAGTTCCCCATGCAAGGAAGTACCCTGCCCCCGGTGAACCTGACAGATGACCAGATAGCCACTGGCCTCTACGTATGTCCAAATAATGAAAACACACTGAAGTCCATCATGAAGAAAAGTGATGGGAATAAAGATTCAAATGGAGCCAAAAAGAATCTTCAGTTCATTGGCATTAACGGC
Peptide sequence:
MGIKIQMEPKRIFSSLALTAEKADGVLNGDHNKEKKDPYFVETPYGFQLDLDFVKYVDDIQKGNTIKKLNIQKRRKPSVPCPEVRAIPGHQGVWTSTESLSSSNSDDSKQCPSFLLARSHVTSTPIPRPPAPLETSPTFAVSENRQLLPPPSPQLPRHNLHVTKTLMETRRRLEQERVTMQMAPGDFRRPRLASFGGMGSTSSLPSFVGSANHSSAIHQLQNGYQGNGDYNSYVPAAPTTSSMGSSVRHSPLSSGISTPVTNVSPMHLQHIREQMAIALKRLKELEEQVRTIPVLQVKISVLQEEKRQLASQLKSQRASSQNEACGVRKRSYSAGNASQLELLARARRGGGELYIDYEEEEMESVEQSTQRIQEFRQLTADMQALERKIQDSSCEVASELRENGQCPSRECKSVAVGSDENMNDVVVYHRDLRPCKDTAVGTVTETRNVGISVTEAMLGVITEADKEIELQQQTIEALKEKIYRLEVQLKETTHDREMTKLKQELQAAGSRKKVDKATMAQPLVFSKLVEALVPTRDQMVGSHVDTRESCVGTSVQTSSVGTSCHPDCKNQVVGSELPMNWWVVKERVAMHDQCVGRSVETCDRSVGVEVSVCETGSNTEASGSDLTLLKTNLNLKDVRSIGCGDCSVDVIVCFPKECTSRSMNTEAVGQEEAAVMAVPHTTDQHTSTNLERVDQCTNTEAATLVESCTNTLLSTMDKQTSTQTVEMRTVAIGEGRVRDINPSTKTRSVGVGTVLSGNSEFDRPCAVKTKESGVGQINIQDNYLVGLKMRTIACGPPQLTVGLMGSRRSVGVGNEPVGELPEESPQPRVASGMVTGLDHYIERVQRLLAEQQTLLAENYSELAEAFGEPHSQIGSLNSQLISTLSSINSVMKSASTEELRNSDFQKASLGKTTGNHLEYTCKCGGLRSGGLLNVQPSQPEVEAETAEGKHSRGHEQFPMQGSTLPPVNLTDDQIATGLYVCPNNENTLKSIMKKSDGNKDSNGAKKNLQFIGING
IRESfinder result
IRESfinder score
0.318969024
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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