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mmu_circKalrn_2523
Basic information of circular RNA
PanCircBase ID:
mmu_circKalrn_2523
mm39_Chr. Position ID:
chr16|33810137|33875485|-
Gene name:
Kalrn
Transcript:
ENSMUST00000114973.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
20
Exon/intron indexes:
21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,3091,3868,19502,20754,24190,26624,38170,38872,41786,42650,43739,44688,45068,45285,45725,50111,60062,63792,65064
Genomic length:
65348
Exon/intron sizes in nt:
12,21,72,28,60,12,42,50,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr16:33809271-33810137|chr16:33875485-33916165
Splice length:
2523
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATTCATACTCTCATTCCTCCAGTGAGAATGGAGGCAAATCCGAGTCCGTGGCCCACCTGCAGTCTCAGCCCTCCCTGAACTCCATCCACAGTTCCCCTGGTCCCAAGCGCTCCACCAACACCCTCAAAAAGTGGCTGACCAGCCCCGTGCGCCGCCTCAACAGCGGAAAAGCCGACGGGAACATCAAAAAGCAGAAGAAAGTCCGGGATGGGCGGAAGAGCTTCGATCTGGGATCGCCCAAGCCTGGGGATGAGACGACACCTCAGGGAGACAGCGCTGACGAGAAGAGCAAGAAGGGTTGGGGCGAAGACGAGCCAGATGAAGAGTCACACACACCCCTCCCTCCGCCCATGAAGATCTTTGACAACGACCCCACACAGGATGAGATGTCGTCTTTGCTAGCAGCCCGGCAGGCTCCCCCTGACGTGCCTACTGCTGCAGACCTTGTCAGTGCAATAGAAAAGTTGGTCAAAAATAAGCTGACTCTGGAAGGAGGCTCTTACCGGGGGAGCTTGAAGGACCCTACAGGCTGCCTGAATGAGGGGATGACCCCTCCCACACCTCCTAGAAACCTGGAAGAAGAACAGAAAGCCAAGGCGCTGAGAGGCAGGATGTTTGTCCTGAATGAGCTGGTACAGACAGAGAAGGACTACGTCAAGGACCTGGGGATTGTGGTGGAGGGCTTCATGAAGAGAATAGAAGAGAAGGGGGTCCCTGAGGACATGCGAGGGAAGGAAAAGATCGTGTTTGGAAATATCCACCAGATTTACGACTGGCATAAAGATTTTTTCCTGGCTGAACTGGAAAAATGTATCCAAGAGCAAGACAGATTGGCCCAGCTCTTCATTAAACACGAGCGGAAGCTGCACATCTACGTGTGGTACTGCCAGAACAAGCCGCGTTCCGAGTACATCGTGGCTGAGTACGATGCCTATTTTGAGGAGGTTAAACAAGAGATCAATCAAAGGCTGACCCTTAGTGACTTCCTGATCAAGCCCATTCAGAGAATAACAAAGTACCAGCTGCTACTCAAGGACTTCCTGCGATACAGCGAGAAGGCTGGTTTGGAGTGTTCCGATATTGAGAAAGCAGTGGAGTTAATGTGCCTTGTCCCCAAGCGCTGTAATGACATGATGAATCTGGGACGCCTGCAGGGCTTCGAGGGCACCCTGACCGCCCAGGGCAAGCTACTGCAGCAGGACACATTCTATGTGATCGAGCTGGATGCCGGCATGCAGTCCCGGACCAAGGAGAGGCGTGTGTTTCTCTTTGAGCAGATTGTCATCTTCAGTGAACTGCTCCGAAAGGGGTCCCTCACCCCAGGCTACATGTTCAAAAGAAGCATCAAGATGAATTACTTGGTCCTGGAAGACAATGTGGATGGTGACCCCTGCAAATTTGCACTCATGAACAGGGAGACTTCAGAAAGGGTCATTCTACAGGCTGCCAACTCAGACATCCAGCAGGCCTGGGTGCAGGACATCAACCAAGTCCTAGAAACACAGAGGGACTTCTTAAATGCACTACAGTCACCCATTGAGTATCAGCGGAAGGAGAGAAGCACAGCTGTGATCCGGTCCCAGCCCCCGAGGGTTCCACAAGCCAGCCCCAGGCCCTACTCTTCTGGCCCGGTGGGCTCAGAGAAGCCCCCCAAGGGCTCCAGCTATAACCCGCCTCTGCCACCCCTGAAGATATCTACCTCTAATGGCAGTCCAGGGTTCGACTACCATCAGCCTGGGGACAAGTTTGACGCTAGCAAGCAGAACGACCTGGGAGGCTGCAATGGGACATCAACCATGACTGTAATCAAAGATTACTATGCACTGAAGGAGAATGAAATCTGCGTGAGCCAAGGTGAGGTGGTACAGGTCCTCGCCGTCAACCAGCAGAACATGTGTCTGGTGTACCAGCCTGCCAGCGACCATTCCCCGGCAGCAGAGGGCTGGGTCCCAGGCAGCATCCTGGCGCCCCTCGCCAAAGCCACAGCAGCGGCCGAAAGTAGTGACGGGAGCATCAAGAAGTCATGCTCTTGGCACACTCTACGCATGAGAAAGCGGGCAGACGTGGAGAACTCGGGTAAAAATGAAGCCACAGGGCCTCGTAAACCCAAGGATATTCTGGGCAACAAAGTCTCTGTTAAAGAGACGAACAGTTCCGAGGAGTCAGAGTGTGATGATCTTGACCCTAATACTAGTATGGAGATCTTGAATCCAAATTTCATCCAGGAAGTGGCTCCAGAATTCCTCGTGCCCTTAGTAGATGTGACCTGTTTGCTTGGAGACACAGTGCTACTGCAGTGTAAAGCCTGTGGGCGGCCAAAGCCCAGCATCACCTGGAAGGGTCCGGATCAGAACATCCTAGACACTGACAACAGTTCAGCAACATACACCATCTCCTCCTGTGATTCTGGGGAAAGCACCCTCAAGATCTGCAACCTGATGCCCCAGGATAGTGGGATTTATACCTGCATAGCAGCAAACGACCACGGGACTGCATCCACATCTGCTACAGTTAAGGTGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATAGCAGCAAACGACCACG
59.9
AGGGGAACTGTGGATGGAGT
60.18
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGATCTGCAACCTGATGCCC
60.11
GGACTCGGATTTGCCTCCAT
59.82
146
PCR template sequence:
AAGATCTGCAACCTGATGCCCCAGGATAGTGGGATTTATACCTGCATAGCAGCAAACGACCACGGGACTGCATCCACATCTGCTACAGTTAAGGTGCAAGATTCATACTCTCATTCCTCCAGTGAGAATGGAGGCAAATCCGAGTCCGTGGCCCACCTGCAGTCTCAGCCCTCCCTGAACTCCATCCACAGTTCCCCTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
27
Interacting miRNAs (#binding sites)
mmu-miR-32-5p(1), mmu-let-7b-5p(1), mmu-miR-122-5p(1), mmu-miR-205-5p(1), mmu-miR-130a-3p(1), mmu-miR-19a-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-30c-5p(1), mmu-miR-298-5p(1), mmu-miR-217-5p(1), mmu-miR-25-3p(1), mmu-miR-139-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-351-5p(1), mmu-miR-130b-3p(1), mmu-miR-721(1), mmu-miR-125b-1-3p(1), mmu-miR-370-3p(1), mmu-miR-297a-5p(1), mmu-miR-224-5p(1), mmu-miR-673-5p(1), mmu-miR-670-5p(1), mmu-miR-697(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTAAGGTGCAAGATTCATACTCTCA
siRNA sets
AGAGTATGAATCTTGCACC, GAGTATGAATCTTGCACCT, ATGAATCTTGCACCTTAAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCKALRN_2523_ORF_1
1.1519
0.398726422
1
4695
ORF sequence:
ATGAAGATCTTTGACAACGACCCCACACAGGATGAGATGTCGTCTTTGCTAGCAGCCCGGCAGGCTCCCCCTGACGTGCCTACTGCTGCAGACCTTGTCAGTGCAATAGAAAAGTTGGTCAAAAATAAGCTGACTCTGGAAGGAGGCTCTTACCGGGGGAGCTTGAAGGACCCTACAGGCTGCCTGAATGAGGGGATGACCCCTCCCACACCTCCTAGAAACCTGGAAGAAGAACAGAAAGCCAAGGCGCTGAGAGGCAGGATGTTTGTCCTGAATGAGCTGGTACAGACAGAGAAGGACTACGTCAAGGACCTGGGGATTGTGGTGGAGGGCTTCATGAAGAGAATAGAAGAGAAGGGGGTCCCTGAGGACATGCGAGGGAAGGAAAAGATCGTGTTTGGAAATATCCACCAGATTTACGACTGGCATAAAGATTTTTTCCTGGCTGAACTGGAAAAATGTATCCAAGAGCAAGACAGATTGGCCCAGCTCTTCATTAAACACGAGCGGAAGCTGCACATCTACGTGTGGTACTGCCAGAACAAGCCGCGTTCCGAGTACATCGTGGCTGAGTACGATGCCTATTTTGAGGAGGTTAAACAAGAGATCAATCAAAGGCTGACCCTTAGTGACTTCCTGATCAAGCCCATTCAGAGAATAACAAAGTACCAGCTGCTACTCAAGGACTTCCTGCGATACAGCGAGAAGGCTGGTTTGGAGTGTTCCGATATTGAGAAAGCAGTGGAGTTAATGTGCCTTGTCCCCAAGCGCTGTAATGACATGATGAATCTGGGACGCCTGCAGGGCTTCGAGGGCACCCTGACCGCCCAGGGCAAGCTACTGCAGCAGGACACATTCTATGTGATCGAGCTGGATGCCGGCATGCAGTCCCGGACCAAGGAGAGGCGTGTGTTTCTCTTTGAGCAGATTGTCATCTTCAGTGAACTGCTCCGAAAGGGGTCCCTCACCCCAGGCTACATGTTCAAAAGAAGCATCAAGATGAATTACTTGGTCCTGGAAGACAATGTGGATGGTGACCCCTGCAAATTTGCACTCATGAACAGGGAGACTTCAGAAAGGGTCATTCTACAGGCTGCCAACTCAGACATCCAGCAGGCCTGGGTGCAGGACATCAACCAAGTCCTAGAAACACAGAGGGACTTCTTAAATGCACTACAGTCACCCATTGAGTATCAGCGGAAGGAGAGAAGCACAGCTGTGATCCGGTCCCAGCCCCCGAGGGTTCCACAAGCCAGCCCCAGGCCCTACTCTTCTGGCCCGGTGGGCTCAGAGAAGCCCCCCAAGGGCTCCAGCTATAACCCGCCTCTGCCACCCCTGAAGATATCTACCTCTAATGGCAGTCCAGGGTTCGACTACCATCAGCCTGGGGACAAGTTTGACGCTAGCAAGCAGAACGACCTGGGAGGCTGCAATGGGACATCAACCATGACTGTAATCAAAGATTACTATGCACTGAAGGAGAATGAAATCTGCGTGAGCCAAGGTGAGGTGGTACAGGTCCTCGCCGTCAACCAGCAGAACATGTGTCTGGTGTACCAGCCTGCCAGCGACCATTCCCCGGCAGCAGAGGGCTGGGTCCCAGGCAGCATCCTGGCGCCCCTCGCCAAAGCCACAGCAGCGGCCGAAAGTAGTGACGGGAGCATCAAGAAGTCATGCTCTTGGCACACTCTACGCATGAGAAAGCGGGCAGACGTGGAGAACTCGGGTAAAAATGAAGCCACAGGGCCTCGTAAACCCAAGGATATTCTGGGCAACAAAGTCTCTGTTAAAGAGACGAACAGTTCCGAGGAGTCAGAGTGTGATGATCTTGACCCTAATACTAGTATGGAGATCTTGAATCCAAATTTCATCCAGGAAGTGGCTCCAGAATTCCTCGTGCCCTTAGTAGATGTGACCTGTTTGCTTGGAGACACAGTGCTACTGCAGTGTAAAGCCTGTGGGCGGCCAAAGCCCAGCATCACCTGGAAGGGTCCGGATCAGAACATCCTAGACACTGACAACAGTTCAGCAACATACACCATCTCCTCCTGTGATTCTGGGGAAAGCACCCTCAAGATCTGCAACCTGATGCCCCAGGATAGTGGGATTTATACCTGCATAGCAGCAAACGACCACGGGACTGCATCCACATCTGCTACAGTTAAGGTGCAAGATTCATACTCTCATTCCTCCAGTGAGAATGGAGGCAAATCCGAGTCCGTGGCCCACCTGCAGTCTCAGCCCTCCCTGAACTCCATCCACAGTTCCCCTGGTCCCAAGCGCTCCACCAACACCCTCAAAAAGTGGCTGACCAGCCCCGTGCGCCGCCTCAACAGCGGAAAAGCCGACGGGAACATCAAAAAGCAGAAGAAAGTCCGGGATGGGCGGAAGAGCTTCGATCTGGGATCGCCCAAGCCTGGGGATGAGACGACACCTCAGGGAGACAGCGCTGACGAGAAGAGCAAGAAGGGTTGGGGCGAAGACGAGCCAGATGAAGAGTCACACACACCCCTCCCTCCGCCCATGAAGATCTTTGACAACGACCCCACACAGGATGAGATGTCGTCTTTGCTAGCAGCCCGGCAGGCTCCCCCTGACGTGCCTACTGCTGCAGACCTTGTCAGTGCAATAGAAAAGTTGGTCAAAAATAAGCTGACTCTGGAAGGAGGCTCTTACCGGGGGAGCTTGAAGGACCCTACAGGCTGCCTGAATGAGGGGATGACCCCTCCCACACCTCCTAGAAACCTGGAAGAAGAACAGAAAGCCAAGGCGCTGAGAGGCAGGATGTTTGTCCTGAATGAGCTGGTACAGACAGAGAAGGACTACGTCAAGGACCTGGGGATTGTGGTGGAGGGCTTCATGAAGAGAATAGAAGAGAAGGGGGTCCCTGAGGACATGCGAGGGAAGGAAAAGATCGTGTTTGGAAATATCCACCAGATTTACGACTGGCATAAAGATTTTTTCCTGGCTGAACTGGAAAAATGTATCCAAGAGCAAGACAGATTGGCCCAGCTCTTCATTAAACACGAGCGGAAGCTGCACATCTACGTGTGGTACTGCCAGAACAAGCCGCGTTCCGAGTACATCGTGGCTGAGTACGATGCCTATTTTGAGGAGGTTAAACAAGAGATCAATCAAAGGCTGACCCTTAGTGACTTCCTGATCAAGCCCATTCAGAGAATAACAAAGTACCAGCTGCTACTCAAGGACTTCCTGCGATACAGCGAGAAGGCTGGTTTGGAGTGTTCCGATATTGAGAAAGCAGTGGAGTTAATGTGCCTTGTCCCCAAGCGCTGTAATGACATGATGAATCTGGGACGCCTGCAGGGCTTCGAGGGCACCCTGACCGCCCAGGGCAAGCTACTGCAGCAGGACACATTCTATGTGATCGAGCTGGATGCCGGCATGCAGTCCCGGACCAAGGAGAGGCGTGTGTTTCTCTTTGAGCAGATTGTCATCTTCAGTGAACTGCTCCGAAAGGGGTCCCTCACCCCAGGCTACATGTTCAAAAGAAGCATCAAGATGAATTACTTGGTCCTGGAAGACAATGTGGATGGTGACCCCTGCAAATTTGCACTCATGAACAGGGAGACTTCAGAAAGGGTCATTCTACAGGCTGCCAACTCAGACATCCAGCAGGCCTGGGTGCAGGACATCAACCAAGTCCTAGAAACACAGAGGGACTTCTTAAATGCACTACAGTCACCCATTGAGTATCAGCGGAAGGAGAGAAGCACAGCTGTGATCCGGTCCCAGCCCCCGAGGGTTCCACAAGCCAGCCCCAGGCCCTACTCTTCTGGCCCGGTGGGCTCAGAGAAGCCCCCCAAGGGCTCCAGCTATAACCCGCCTCTGCCACCCCTGAAGATATCTACCTCTAATGGCAGTCCAGGGTTCGACTACCATCAGCCTGGGGACAAGTTTGACGCTAGCAAGCAGAACGACCTGGGAGGCTGCAATGGGACATCAACCATGACTGTAATCAAAGATTACTATGCACTGAAGGAGAATGAAATCTGCGTGAGCCAAGGTGAGGTGGTACAGGTCCTCGCCGTCAACCAGCAGAACATGTGTCTGGTGTACCAGCCTGCCAGCGACCATTCCCCGGCAGCAGAGGGCTGGGTCCCAGGCAGCATCCTGGCGCCCCTCGCCAAAGCCACAGCAGCGGCCGAAAGTAGTGACGGGAGCATCAAGAAGTCATGCTCTTGGCACACTCTACGCATGAGAAAGCGGGCAGACGTGGAGAACTCGGGTAAAAATGAAGCCACAGGGCCTCGTAAACCCAAGGATATTCTGGGCAACAAAGTCTCTGTTAAAGAGACGAACAGTTCCGAGGAGTCAGAGTGTGATGATCTTGACCCTAATACTAGTATGGAGATCTTGAATCCAAATTTCATCCAGGAAGTGGCTCCAGAATTCCTCGTGCCCTTAGTAGATGTGACCTGTTTGCTTGGAGACACAGTGCTACTGCAGTGTAAAGCCTGTGGGCGGCCAAAGCCCAGCATCACCTGGAAGGGTCCGGATCAGAACATCCTAGACACTGACAACAGTTCAGCAACATACACCATCTCCTCCTGTGATTCTGGGGAAAGCACCCTCAAGATCTGCAACCTGATGCCCCAGGATAGTGGGATTTATACCTGCATAGCAGCAAACGACCACGGGACTGCATCCACATCTGCTACAGTTAAGGTGCAA
Peptide sequence:
MKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQDSYSHSSSENGGKSESVAHLQSQPSLNSIHSSPGPKRSTNTLKKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLAARQAPPDVPTAADLVSAIEKLVKNKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKEKIVFGNIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEINQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEDNVDGDPCKFALMNRETSERVILQAANSDIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVIRSQPPRVPQASPRPYSSGPVGSEKPPKGSSYNPPLPPLKISTSNGSPGFDYHQPGDKFDASKQNDLGGCNGTSTMTVIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLAKATAAAESSDGSIKKSCSWHTLRMRKRADVENSGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQEVAPEFLVPLVDVTCLLGDTVLLQCKACGRPKPSITWKGPDQNILDTDNSSATYTISSCDSGESTLKICNLMPQDSGIYTCIAANDHGTASTSATVKVQ
IRESfinder result
IRESfinder score
0.417686637
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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