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mmu_circItpkb_693
Basic information of circular RNA
PanCircBase ID:
mmu_circItpkb_693
mm39_Chr. Position ID:
chr1|180240238|180248911|+
Gene name:
Itpkb
Transcript:
ENSMUST00000070181.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,1113,5480,7584,8601
Genomic length:
8673
Exon/intron sizes in nt:
100,214,205,102,72
Flanking exon/introns:
chr1:180161795-180240238|chr1:180248911-180249239
Splice length:
693
Other database IDs:
circAtlas: hsa-ITPKB_0001
Human Ortholog IDs:
Mature spliced Sequence:
AGCAAGTCATGGAGAAAGATAAAGAACATGGTGCAGTGGTCCCCCTTTGTCATGTCCTTCAAGAAGAAGTACCCCTGGATCCAGCTGGCAGGACATGCAGGGAGCTTCAAGGCAGCTGCAAATGGCCGCATCCTTAAGAAGCACTGTGAGTCTGAGCAGCGCTGCCTGGACCGGTTAATGGCGGATGTGCTGAGACCCTTCGTGCCAGCCTACCATGGGGACGTGGTGAAGGATGGGGAACGCTACAACCAGATGGACGACCTGCTGGCTGACTTCGATTCGCCCTGCGTGATGGACTGCAAGATGGGTGTCAGGACATACCTGGAGGAAGAACTCACCAAGGCCCGGAAGAAGCCTAGCTTGCGGAAGGACATGTACCAGAAGATGGTCGAGGTGGACCCTGAGGCCCCCACTGAAGAGGAGAAAGCCCAGCGAGCTGTGACCAAGCCACGTTACATGCAGTGGCGGGAAACCATCAGTTCGACAGCCACCCTAGGCTTCAGGATCGAAGGCATCAAGAAGGAAGATGGCTCTGTGAACCGTGACTTCAAGAAGACCAAAACAAGGGAGCAGGTCACCGAGGCTTTCAGAGAATTCACTAAGGGAAACCAGAACATCTTGATCGCCTACCGGGACCGGCTGAAGGCCATTCGAGCGACCCTGGAAATCTCTCCCTTCTTCAAGTGCCACGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATCTTGATCGCCTACCGGG
60.32
AGCTGGATCCAGGGGTACTT
59.96
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTTGATCGCCTACCGG
59.61
AGCTGGATCCAGGGGTACTT
59.96
166
PCR template sequence:
ATTCACTAAGGGAAACCAGAACATCTTGATCGCCTACCGGGACCGGCTGAAGGCCATTCGAGCGACCCTGGAAATCTCTCCCTTCTTCAAGTGCCACGAGAGCAAGTCATGGAGAAAGATAAAGAACATGGTGCAGTGGTCCCCCTTTGTCATGTCCTTCAAGAAGAAGTACCCCTGGATCCAGCTGGCAGGACATGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(1), mmu-miR-431-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-22-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAGTGCCACGAGAGCAAGTCATGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCITPKB_693_ORF_1
1.2756
0.568315909
0.999901686
1359
ORF sequence:
ATGGTGCAGTGGTCCCCCTTTGTCATGTCCTTCAAGAAGAAGTACCCCTGGATCCAGCTGGCAGGACATGCAGGGAGCTTCAAGGCAGCTGCAAATGGCCGCATCCTTAAGAAGCACTGTGAGTCTGAGCAGCGCTGCCTGGACCGGTTAATGGCGGATGTGCTGAGACCCTTCGTGCCAGCCTACCATGGGGACGTGGTGAAGGATGGGGAACGCTACAACCAGATGGACGACCTGCTGGCTGACTTCGATTCGCCCTGCGTGATGGACTGCAAGATGGGTGTCAGGACATACCTGGAGGAAGAACTCACCAAGGCCCGGAAGAAGCCTAGCTTGCGGAAGGACATGTACCAGAAGATGGTCGAGGTGGACCCTGAGGCCCCCACTGAAGAGGAGAAAGCCCAGCGAGCTGTGACCAAGCCACGTTACATGCAGTGGCGGGAAACCATCAGTTCGACAGCCACCCTAGGCTTCAGGATCGAAGGCATCAAGAAGGAAGATGGCTCTGTGAACCGTGACTTCAAGAAGACCAAAACAAGGGAGCAGGTCACCGAGGCTTTCAGAGAATTCACTAAGGGAAACCAGAACATCTTGATCGCCTACCGGGACCGGCTGAAGGCCATTCGAGCGACCCTGGAAATCTCTCCCTTCTTCAAGTGCCACGAGAGCAAGTCATGGAGAAAGATAAAGAACATGGTGCAGTGGTCCCCCTTTGTCATGTCCTTCAAGAAGAAGTACCCCTGGATCCAGCTGGCAGGACATGCAGGGAGCTTCAAGGCAGCTGCAAATGGCCGCATCCTTAAGAAGCACTGTGAGTCTGAGCAGCGCTGCCTGGACCGGTTAATGGCGGATGTGCTGAGACCCTTCGTGCCAGCCTACCATGGGGACGTGGTGAAGGATGGGGAACGCTACAACCAGATGGACGACCTGCTGGCTGACTTCGATTCGCCCTGCGTGATGGACTGCAAGATGGGTGTCAGGACATACCTGGAGGAAGAACTCACCAAGGCCCGGAAGAAGCCTAGCTTGCGGAAGGACATGTACCAGAAGATGGTCGAGGTGGACCCTGAGGCCCCCACTGAAGAGGAGAAAGCCCAGCGAGCTGTGACCAAGCCACGTTACATGCAGTGGCGGGAAACCATCAGTTCGACAGCCACCCTAGGCTTCAGGATCGAAGGCATCAAGAAGGAAGATGGCTCTGTGAACCGTGACTTCAAGAAGACCAAAACAAGGGAGCAGGTCACCGAGGCTTTCAGAGAATTCACTAAGGGAAACCAGAACATCTTGATCGCCTACCGGGACCGGCTGAAGGCCATTCGAGCGACCCTGGAAATCTCTCCCTTCTTCAAGTGCCACGAG
Peptide sequence:
MVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGVRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRATLEISPFFKCHESKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGVRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRATLEISPFFKCHE
IRESfinder result
IRESfinder score
0.265003199
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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