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mmu_circIqce_391_1
Basic information of circular RNA
PanCircBase ID:
mmu_circIqce_391_1
mm39_Chr. Position ID:
chr5|140660091|140663933|-
Gene name:
Iqce
Transcript:
ENSMUST00000041783.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
18,17,16,15
Exon/intron offsets:
0,741,3079,3737
Genomic length:
3842
Exon/intron sizes in nt:
2,41,71,91,105
Flanking exon/introns:
chr5:140657681-140660091|chr5:140663933-140666470
Splice length:
391
Other database IDs:
circAtlas: mmu-Iqce_0014
Human Ortholog IDs:
Mature spliced Sequence:
CCTGGAGATGAAGCAGCTGCTACAGTCCAAGATCGACCTGGAGAAGGAGTTGGAGACAGCCAGGGAAGGCGAGAAGGGGAGGCAGGAGCAGGAGCAGGCTTTGAGGGAGGAAGTTGAAGCACTCACCAAAAAATGTCAAGAACTGGAAGAAGCTAAGAGAGAAGAGAAAAACTCCTTCGTAGCAGTGACTCATGAGGCTCATCCAGAGCTCCATGCCCCCTCGCCCTGCAGCAGGCACTCCGAGCCAGACTCTGACAATAGTGCTGGTGAGGAGGGTAGCTCCCAACCCCCTGCCCCATGCTCTGAAGAGAGGAGAGAGGCTGCCATTAGGACCCTGCAGGCGCAGTGGAAGGCCCACAGGCGCAAGAAAAGAGAAGCAGCTCTGGATGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGAGGCTGCCATTAGGACC
59.89
CTTCCCTGGCTGTCTCCAAC
60.32
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGGAGAGAGGCTGCCATT
60.33
CTTCCCTGGCTGTCTCCAAC
60.32
151
PCR template sequence:
TGCCCCATGCTCTGAAGAGAGGAGAGAGGCTGCCATTAGGACCCTGCAGGCGCAGTGGAAGGCCCACAGGCGCAAGAAAAGAGAAGCAGCTCTGGATGAGCCTGGAGATGAAGCAGCTGCTACAGTCCAAGATCGACCTGGAGAAGGAGTTGGAGACAGCCAGGGAAGGCGAGAAGGGGAGGCAGGAGCAGGAGCAGGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(1), mmu-miR-129-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCTCTGGATGAGCCTGGAGATGAAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCIQCE_391_1_ORF_1
1.0056
0.28527653
0.884920769
591
ORF sequence:
ATGAGGCTCATCCAGAGCTCCATGCCCCCTCGCCCTGCAGCAGGCACTCCGAGCCAGACTCTGACAATAGTGCTGGTGAGGAGGGTAGCTCCCAACCCCCTGCCCCATGCTCTGAAGAGAGGAGAGAGGCTGCCATTAGGACCCTGCAGGCGCAGTGGAAGGCCCACAGGCGCAAGAAAAGAGAAGCAGCTCTGGATGAGCCTGGAGATGAAGCAGCTGCTACAGTCCAAGATCGACCTGGAGAAGGAGTTGGAGACAGCCAGGGAAGGCGAGAAGGGGAGGCAGGAGCAGGAGCAGGCTTTGAGGGAGGAAGTTGAAGCACTCACCAAAAAATGTCAAGAACTGGAAGAAGCTAAGAGAGAAGAGAAAAACTCCTTCGTAGCAGTGACTCATGAGGCTCATCCAGAGCTCCATGCCCCCTCGCCCTGCAGCAGGCACTCCGAGCCAGACTCTGACAATAGTGCTGGTGAGGAGGGTAGCTCCCAACCCCCTGCCCCATGCTCTGAAGAGAGGAGAGAGGCTGCCATTAGGACCCTGCAGGCGCAGTGGAAGGCCCACAGGCGCAAGAAAAGAGAAGCAGCTCTGGATGAG
Peptide sequence:
MRLIQSSMPPRPAAGTPSQTLTIVLVRRVAPNPLPHALKRGERLPLGPCRRSGRPTGARKEKQLWMSLEMKQLLQSKIDLEKELETAREGEKGRQEQEQALREEVEALTKKCQELEEAKREEKNSFVAVTHEAHPELHAPSPCSRHSEPDSDNSAGEEGSSQPPAPCSEERREAAIRTLQAQWKAHRRKKREAALDE
IRESfinder result
IRESfinder score
0.269697033
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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