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mmu_circInts4_368
Basic information of circular RNA
PanCircBase ID:
mmu_circInts4_368
mm39_Chr. Position ID:
chr7|97150934|97157097|+
Gene name:
Ints4
Transcript:
ENSMUST00000026126.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
8,9,10
Exon/intron offsets:
0,4336,5988
Genomic length:
6163
Exon/intron sizes in nt:
1,21,72,175
Flanking exon/introns:
chr7:97150265-97150934|chr7:97157097-97158797
Splice length:
368
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TATAGTTCCAATACCGTCTTCTAATGAAGAAATCCGTCTAGTTGATGATGCATTTGGAAAAATTTGCCACATGGTCAGTGATGGCTCCTGGGTGGTTCGTGTTCAAGCTGCAAAGCTATTGGGCTCGATGGAGCAAGTTAGCTCTCATTTCTTGGAGCAGACTCTTGACAAGAAGCTGATGTCAGATCTGAGGAGGAAACGCACTGCACATGAGCGTGCCAAGGAACTTTACAGTTCGGGGGAGTTTTCCAGTGGCAGGAAATGGGGAGATGATGCTCCCAAGGAAGAAATAGACACGGGGGCCGTGAACCTGATAGAGTCAGGAGCGTGTGGAGCCTTTGTTCATGGGTTGGAAGATGAGATGTATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAGAGTCAGGAGCGTGTGGA
59.96
AGCCATCACTGACCATGTGG
60.03
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGCCGTGAACCTGATAGAG
59.89
AGCCATCACTGACCATGTGG
60.03
155
PCR template sequence:
GATGATGCTCCCAAGGAAGAAATAGACACGGGGGCCGTGAACCTGATAGAGTCAGGAGCGTGTGGAGCCTTTGTTCATGGGTTGGAAGATGAGATGTATGTATAGTTCCAATACCGTCTTCTAATGAAGAAATCCGTCTAGTTGATGATGCATTTGGAAAAATTTGCCACATGGTCAGTGATGGCTCCTGGGTGGTTCGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-96-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGAGATGTATGTATAGTTCCAATA
siRNA sets
TATTGGAACTATACATACA,GAACTATACATACATCTCA,AACTATACATACATCTCAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCINTS4_368_ORF_1
0.9764
0.176958141
0.36946417
303
ORF sequence:
ATGGTCAGTGATGGCTCCTGGGTGGTTCGTGTTCAAGCTGCAAAGCTATTGGGCTCGATGGAGCAAGTTAGCTCTCATTTCTTGGAGCAGACTCTTGACAAGAAGCTGATGTCAGATCTGAGGAGGAAACGCACTGCACATGAGCGTGCCAAGGAACTTTACAGTTCGGGGGAGTTTTCCAGTGGCAGGAAATGGGGAGATGATGCTCCCAAGGAAGAAATAGACACGGGGGCCGTGAACCTGATAGAGTCAGGAGCGTGTGGAGCCTTTGTTCATGGGTTGGAAGATGAGATGTATGTATAG
Peptide sequence:
MVSDGSWVVRVQAAKLLGSMEQVSSHFLEQTLDKKLMSDLRRKRTAHERAKELYSSGEFSSGRKWGDDAPKEEIDTGAVNLIESGACGAFVHGLEDEMYV
IRESfinder result
IRESfinder score
0.368851335
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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