top of page
mmu_circInsr_912
Basic information of circular RNA
PanCircBase ID:
mmu_circInsr_912
mm39_Chr. Position ID:
chr8|3239124|3253034|-
Gene name:
Insr
Transcript:
ENSMUST00000091291.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
9,8,7,6,5
Exon/intron offsets:
0,3421,5670,8936,13765
Genomic length:
13910
Exon/intron sizes in nt:
16,82,57,12,72,15,145
Flanking exon/introns:
chr8:3235152-3239124|chr8:3253034-3254629
Splice length:
912
Other database IDs:
CIRCpedia: MMU_CIRCpedia_218988, circAtlas: mmu-Insr_0008
circAtlas: hsa-INSR_0014
Human Ortholog IDs:
Mature spliced Sequence:
ACAACCTGGCAGCTGAGCTGGAGGCTAACCTTGGCCTCATTGAAGAAATTTCGGGATTTCTAAAGATCCGCCGCTCCTATGCTCTGGTATCACTTTCTTTCTTCAGGAAGCTACATCTGATTCGAGGAGAGACCTTGGAAATTGGGAACTATTCTTTTTATGCCTTGGACAACCAGAACCTGAGGCAACTCTGGGACTGGAGCAAACACAACCTCACCATCACTCAGGGCAAGCTCTTCTTCCATTACAACCCGAAACTCTGCTTGTCTGAAATTCACAAGATGGAAGAAGTCTCCGGAACTAAGGGCCGTCAGGAGAGGAACGACATTGCCCTGAAGACCAATGGGGACCAGGCATCGTGTGAAAATGAATTGCTTAAATTTTCTTTCATTCGGACATCTTTTGACAAGATCCTGTTGAGGTGGGAACCCTACTGGCCCCCCGACTTCCGAGATCTCCTGGGATTCATGTTGTTCTACAAAGAGGCCCCTTATCAGAATGTGACAGAGTTTGATGGGCAGGATGCTTGTGGCTCCAACAGCTGGACTGTGGTGGATATTGACCCGCCCCAGAGGTCCAACGACCCCAAGTCTCAGACCCCAAGCCACCCTGGGTGGCTGATGCGGGGCCTCAAACCCTGGACCCAATACGCCATCTTTGTGAAGACCTTGGTTACCTTCTCTGATGAACGGCGGACCTATGGAGCCAAAAGTGATATCATCTATGTGCAAACAGATGCCACTAATCCTTCTGTCCCCCTGGATCCCATATCAGTTTCTAATTCCTCATCTCAGATTATCTTAAAGTGGAAGCCCCCCTCTGACCCCAATGGCAACATCACACACTACCTGGTGTACTGGGAGAGGCAAGCAGAGGACAGCGAGCTGTTTGAGCTGGATTATTGTCTCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTACTGGGAGAGGCAAGCAG
60.11
CATAGGAGCGGCGGATCTTT
59.97
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGTACTGGGAGAGGCAAG
59.75
CATAGGAGCGGCGGATCTTT
59.97
143
PCR template sequence:
CCCCCCTCTGACCCCAATGGCAACATCACACACTACCTGGTGTACTGGGAGAGGCAAGCAGAGGACAGCGAGCTGTTTGAGCTGGATTATTGTCTCAAAGACAACCTGGCAGCTGAGCTGGAGGCTAACCTTGGCCTCATTGAAGAAATTTCGGGATTTCTAAAGATCCGCCGCTCCTATGCTCTGGTATCACTTTCTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-196a-5p(1), mmu-miR-143-3p(1), mmu-miR-22-3p(1), mmu-miR-680(1), mmu-miR-207(1), mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TATTGTCTCAAAGACAACCTGGCAGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCINSR_912_ORF_1
0.9434
0.3102584
0.999858734
1542
ORF sequence:
ATGGAAGAAGTCTCCGGAACTAAGGGCCGTCAGGAGAGGAACGACATTGCCCTGAAGACCAATGGGGACCAGGCATCGTGTGAAAATGAATTGCTTAAATTTTCTTTCATTCGGACATCTTTTGACAAGATCCTGTTGAGGTGGGAACCCTACTGGCCCCCCGACTTCCGAGATCTCCTGGGATTCATGTTGTTCTACAAAGAGGCCCCTTATCAGAATGTGACAGAGTTTGATGGGCAGGATGCTTGTGGCTCCAACAGCTGGACTGTGGTGGATATTGACCCGCCCCAGAGGTCCAACGACCCCAAGTCTCAGACCCCAAGCCACCCTGGGTGGCTGATGCGGGGCCTCAAACCCTGGACCCAATACGCCATCTTTGTGAAGACCTTGGTTACCTTCTCTGATGAACGGCGGACCTATGGAGCCAAAAGTGATATCATCTATGTGCAAACAGATGCCACTAATCCTTCTGTCCCCCTGGATCCCATATCAGTTTCTAATTCCTCATCTCAGATTATCTTAAAGTGGAAGCCCCCCTCTGACCCCAATGGCAACATCACACACTACCTGGTGTACTGGGAGAGGCAAGCAGAGGACAGCGAGCTGTTTGAGCTGGATTATTGTCTCAAAGACAACCTGGCAGCTGAGCTGGAGGCTAACCTTGGCCTCATTGAAGAAATTTCGGGATTTCTAAAGATCCGCCGCTCCTATGCTCTGGTATCACTTTCTTTCTTCAGGAAGCTACATCTGATTCGAGGAGAGACCTTGGAAATTGGGAACTATTCTTTTTATGCCTTGGACAACCAGAACCTGAGGCAACTCTGGGACTGGAGCAAACACAACCTCACCATCACTCAGGGCAAGCTCTTCTTCCATTACAACCCGAAACTCTGCTTGTCTGAAATTCACAAGATGGAAGAAGTCTCCGGAACTAAGGGCCGTCAGGAGAGGAACGACATTGCCCTGAAGACCAATGGGGACCAGGCATCGTGTGAAAATGAATTGCTTAAATTTTCTTTCATTCGGACATCTTTTGACAAGATCCTGTTGAGGTGGGAACCCTACTGGCCCCCCGACTTCCGAGATCTCCTGGGATTCATGTTGTTCTACAAAGAGGCCCCTTATCAGAATGTGACAGAGTTTGATGGGCAGGATGCTTGTGGCTCCAACAGCTGGACTGTGGTGGATATTGACCCGCCCCAGAGGTCCAACGACCCCAAGTCTCAGACCCCAAGCCACCCTGGGTGGCTGATGCGGGGCCTCAAACCCTGGACCCAATACGCCATCTTTGTGAAGACCTTGGTTACCTTCTCTGATGAACGGCGGACCTATGGAGCCAAAAGTGATATCATCTATGTGCAAACAGATGCCACTAATCCTTCTGTCCCCCTGGATCCCATATCAGTTTCTAATTCCTCATCTCAGATTATCTTAAAGTGGAAGCCCCCCTCTGACCCCAATGGCAACATCACACACTACCTGGTGTACTGGGAGAGGCAAGCAGAGGACAGCGAGCTGTTTGAGCTGGATTATTGTCTCAAA
Peptide sequence:
MEEVSGTKGRQERNDIALKTNGDQASCENELLKFSFIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPQRSNDPKSQTPSHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVYWERQAEDSELFELDYCLKDNLAAELEANLGLIEEISGFLKIRRSYALVSLSFFRKLHLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSFIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPQRSNDPKSQTPSHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVYWERQAEDSELFELDYCLK
IRESfinder result
IRESfinder score
0.651315834
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page