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mmu_circInsig2_392
Basic information of circular RNA
PanCircBase ID:
mmu_circInsig2_392
mm39_Chr. Position ID:
chr1|121234592|121240054|-
Gene name:
Insig2
Transcript:
ENSMUST00000003818.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,276,5337
Genomic length:
5462
Exon/intron sizes in nt:
10,01,67,125
Flanking exon/introns:
chr1:121233676-121234592|chr1:121240054-121247325
Splice length:
392
Other database IDs:
circAtlas: mmu-Insig2_0004
Human Ortholog IDs:
Mature spliced Sequence:
CTGTGATTGGGCTGTTGTACCCCTGCATTGACAGGCATCTAGGAGAACCTCATAAATTTAAAAGAGAGTGGTCCAGTGTCATGCGCTGCGTGGCGGTGTTCGTGGGTATAAATCACGCCAGTGCTAAAGTAGACTTCGACAACAACTTCCAGTTTTCCCTCACACTGGCTGCACTGTCAGTAGGACTGTGGTGGACTTTTGACAGATCTAGAAGTGGTTTTGGCCTTGGTGTTGGAATTGCTTTCTTAGCAACCGTTGTCACCCAACTGTTAGTCTACAATGGTGTTTATCAATACACATCTCCAGATTTTCTCTATGTCCGTTCTTGGTTGCCATGTATATTTTTTGCTGGAGGCATAACGATGGGAAACATTGGCCGTCAACTGGCAATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCGTTCTTGGTTGCCATGT
60.18
GCATGACACTGGACCACTCT
59.68
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCCGTTCTTGGTTGCCAT
60.18
GCATGACACTGGACCACTCT
59.68
160
PCR template sequence:
ATACACATCTCCAGATTTTCTCTATGTCCGTTCTTGGTTGCCATGTATATTTTTTGCTGGAGGCATAACGATGGGAAACATTGGCCGTCAACTGGCAATGCTGTGATTGGGCTGTTGTACCCCTGCATTGACAGGCATCTAGGAGAACCTCATAAATTTAAAAGAGAGTGGTCCAGTGTCATGCGCTGCGTGGCGGTGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-34c-5p(1), mmu-miR-382-5p(1), mmu-miR-511-3p(1), mmu-miR-425-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAACTGGCAATGCTGTGATTGGGCT
siRNA sets
AGCCCAATCACAGCATTGC,GCCCAATCACAGCATTGCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCINSIG2_392_ORF_1
0.5625
-0.041498014
0.242735386
450
ORF sequence:
ATGTATATTTTTTGCTGGAGGCATAACGATGGGAAACATTGGCCGTCAACTGGCAATGCTGTGATTGGGCTGTTGTACCCCTGCATTGACAGGCATCTAGGAGAACCTCATAAATTTAAAAGAGAGTGGTCCAGTGTCATGCGCTGCGTGGCGGTGTTCGTGGGTATAAATCACGCCAGTGCTAAAGTAGACTTCGACAACAACTTCCAGTTTTCCCTCACACTGGCTGCACTGTCAGTAGGACTGTGGTGGACTTTTGACAGATCTAGAAGTGGTTTTGGCCTTGGTGTTGGAATTGCTTTCTTAGCAACCGTTGTCACCCAACTGTTAGTCTACAATGGTGTTTATCAATACACATCTCCAGATTTTCTCTATGTCCGTTCTTGGTTGCCATGTATATTTTTTGCTGGAGGCATAACGATGGGAAACATTGGCCGTCAACTGGCAATG
Peptide sequence:
MYIFCWRHNDGKHWPSTGNAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNFQFSLTLAALSVGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQLAM
IRESfinder result
IRESfinder score
0.708637674
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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