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mmu_circInpp5e_729
Basic information of circular RNA
PanCircBase ID:
mmu_circInpp5e_729
mm39_Chr. Position ID:
chr2|26289244|26291710|-
Gene name:
Inpp5e
Transcript:
ENSMUST00000145701.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
8,7,6,5,4,3
Exon/intron offsets:
0,180,803,1140,1653,2368
Genomic length:
2466
Exon/intron sizes in nt:
116,162,108,120,125,98
Flanking exon/introns:
chr2:26288704-26289244|chr2:26291710-26292111
Splice length:
729
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAGCTCCCAGCGAGCCTGGATGAGTTTCTGCTCCCCACCGAGGCTGACTACACTCAGGACCTGTATGTCATTGGAATTCAGGAGGGCTGCTCTGACAGGCGGGAGTGGGAGACACGCCTGCAGGAGACACTGGGCCCTCAGTATGTACTGCTGTCATCAGCAGCACATGGGGTCCTGTACATGTCCCTGTTTATCCGTAGGGACCTCATCTGGTTCTGCTCAGAGGTCGAGTACTCCACAGTAACTACACGCATCGTGTCTCAGATCAAGACCAAGGGGGCCCTGGGCGTCAGCTTCACCTTTTTTGGCACCTCCTTCCTCTTCATCACATCTCACTTCACCTCTGGAGATGGGAAGGTAGCAGAGCGGCTACTGGACTACAGCAGAACCATCCAAGCCCTAGCCCTGCCCCGGAATGTGCCAGACACAAATCCCTACCGCTCTAGTGCAGGGGATGTCACTACCCGGTTTGATGAGGTCTTCTGGTTTGGGGACTTCAACTTCCGCCTGAGTGGTGGACGAGTGGCTGTGGAGGCCTTCCTGAAGCAGAAACCTGAGGTGGATGTGCTGGCTCTCCTCCAACACGACCAGCTCACCCGGGAGATGAAGAAAGGGTCCATCTTCAGGGGCTTTGAGGAGGCAGAGATTCACTTTCTTCCATCCTACAAGTTTGACATTGGGAAGGACACCTACGACAGCACCTCCAAGCAAAGGACACCCTCCTACACA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGGACACCTACGACAGC
60.11
CAGAGCAGCCCTCCTGAATT
59.74
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAAGGACACCTACGACAGC
60.11
GCAGCCCTCCTGAATTCCAA
60.32
140
PCR template sequence:
CTTTGAGGAGGCAGAGATTCACTTTCTTCCATCCTACAAGTTTGACATTGGGAAGGACACCTACGACAGCACCTCCAAGCAAAGGACACCCTCCTACACAGAGCTCCCAGCGAGCCTGGATGAGTTTCTGCTCCCCACCGAGGCTGACTACACTCAGGACCTGTATGTCATTGGAATTCAGGAGGGCTGCTCTGACAGGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-127-5p(1), mmu-miR-541-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
ACCCTCCTACACAGAGCTCCCAGCGA
siRNA sets
GCTGGGAGCTCTGTGTAGG,CTGGGAGCTCTGTGTAGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCINPP5E_729_ORF_1
1.225
0.45074772
0.999686143
1278
ORF sequence:
ATGTCCCTGTTTATCCGTAGGGACCTCATCTGGTTCTGCTCAGAGGTCGAGTACTCCACAGTAACTACACGCATCGTGTCTCAGATCAAGACCAAGGGGGCCCTGGGCGTCAGCTTCACCTTTTTTGGCACCTCCTTCCTCTTCATCACATCTCACTTCACCTCTGGAGATGGGAAGGTAGCAGAGCGGCTACTGGACTACAGCAGAACCATCCAAGCCCTAGCCCTGCCCCGGAATGTGCCAGACACAAATCCCTACCGCTCTAGTGCAGGGGATGTCACTACCCGGTTTGATGAGGTCTTCTGGTTTGGGGACTTCAACTTCCGCCTGAGTGGTGGACGAGTGGCTGTGGAGGCCTTCCTGAAGCAGAAACCTGAGGTGGATGTGCTGGCTCTCCTCCAACACGACCAGCTCACCCGGGAGATGAAGAAAGGGTCCATCTTCAGGGGCTTTGAGGAGGCAGAGATTCACTTTCTTCCATCCTACAAGTTTGACATTGGGAAGGACACCTACGACAGCACCTCCAAGCAAAGGACACCCTCCTACACAGAGCTCCCAGCGAGCCTGGATGAGTTTCTGCTCCCCACCGAGGCTGACTACACTCAGGACCTGTATGTCATTGGAATTCAGGAGGGCTGCTCTGACAGGCGGGAGTGGGAGACACGCCTGCAGGAGACACTGGGCCCTCAGTATGTACTGCTGTCATCAGCAGCACATGGGGTCCTGTACATGTCCCTGTTTATCCGTAGGGACCTCATCTGGTTCTGCTCAGAGGTCGAGTACTCCACAGTAACTACACGCATCGTGTCTCAGATCAAGACCAAGGGGGCCCTGGGCGTCAGCTTCACCTTTTTTGGCACCTCCTTCCTCTTCATCACATCTCACTTCACCTCTGGAGATGGGAAGGTAGCAGAGCGGCTACTGGACTACAGCAGAACCATCCAAGCCCTAGCCCTGCCCCGGAATGTGCCAGACACAAATCCCTACCGCTCTAGTGCAGGGGATGTCACTACCCGGTTTGATGAGGTCTTCTGGTTTGGGGACTTCAACTTCCGCCTGAGTGGTGGACGAGTGGCTGTGGAGGCCTTCCTGAAGCAGAAACCTGAGGTGGATGTGCTGGCTCTCCTCCAACACGACCAGCTCACCCGGGAGATGAAGAAAGGGTCCATCTTCAGGGGCTTTGAGGAGGCAGAGATTCACTTTCTTCCATCCTACAAGTTTGACATTGGGAAGGACACCTACGACAGCACCTCCAAGCAAAGGACACCCTCCTACACA
Peptide sequence:
MSLFIRRDLIWFCSEVEYSTVTTRIVSQIKTKGALGVSFTFFGTSFLFITSHFTSGDGKVAERLLDYSRTIQALALPRNVPDTNPYRSSAGDVTTRFDEVFWFGDFNFRLSGGRVAVEAFLKQKPEVDVLALLQHDQLTREMKKGSIFRGFEEAEIHFLPSYKFDIGKDTYDSTSKQRTPSYTELPASLDEFLLPTEADYTQDLYVIGIQEGCSDRREWETRLQETLGPQYVLLSSAAHGVLYMSLFIRRDLIWFCSEVEYSTVTTRIVSQIKTKGALGVSFTFFGTSFLFITSHFTSGDGKVAERLLDYSRTIQALALPRNVPDTNPYRSSAGDVTTRFDEVFWFGDFNFRLSGGRVAVEAFLKQKPEVDVLALLQHDQLTREMKKGSIFRGFEEAEIHFLPSYKFDIGKDTYDSTSKQRTPSYT
IRESfinder result
IRESfinder score
0.587216213
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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