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mmu_circInpp5b_381
Basic information of circular RNA
PanCircBase ID:
mmu_circInpp5b_381
mm39_Chr. Position ID:
chr4|124645104|124645485|+
Gene name:
Inpp5b
Transcript:
ENSMUST00000094782.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
7
Exon/intron offsets:
0
Genomic length:
381
Exon/intron sizes in nt:
381
Flanking exon/introns:
chr4:124639975-124645104|chr4:124645485-124671133
Splice length:
381
Other database IDs:
circAtlas: mmu-Inpp5b_0011
Human Ortholog IDs:
Mature spliced Sequence:
GTTTCGACCCTGAGACGCGGGATCCCGAGTTCGAGTGGCTGTCAAGACACACATGCGCGGAGCCCGATGCCGAGTCGCCGAAACCGCGCGAGTGGAATTCGGACCCGGGTACCCGGAGTGGGTTCGCGCCCATTGGAGGAAGCAGGCATCAGTCTCGGAATGCACGGCGAGGATTGGAGGATGTCTTGCCTAGGGGCCCTGGCTACATTCTGCTTTGGGGCGGAGCCGCTGAGGAGCCGGAATTTCTATTGGCGGAGGAGATGCATGAGGGCGGGCCTGTGAGAGGACGGAGACCGCTCGCCGGGCGGCGGGATGAAGCGCTTGAGGAAGCAGACTGGGAAATGTCGGCAGGCGGCGGCTCTCGGGAGCGCGACTGTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGACTGGGAAATGTCGGCAG
60.04
AATTCCACTCGCGCGGTTTC
61.91
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGACTGGGAAATGTCGGCAG
60.04
GAATTCCACTCGCGCGGTTT
61.91
150
PCR template sequence:
AGAGGACGGAGACCGCTCGCCGGGCGGCGGGATGAAGCGCTTGAGGAAGCAGACTGGGAAATGTCGGCAGGCGGCGGCTCTCGGGAGCGCGACTGTGCAGGTTTCGACCCTGAGACGCGGGATCCCGAGTTCGAGTGGCTGTCAAGACACACATGCGCGGAGCCCGATGCCGAGTCGCCGAAACCGCGCGAGTGGAATTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-466a-5p(1), mmu-miR-466p-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CGCGACTGTGCAGGTTTCGACCCTGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCINPP5B_381_ORF_1
0.7218
0.301745916
0.717160022
501
ORF sequence:
ATGCATGAGGGCGGGCCTGTGAGAGGACGGAGACCGCTCGCCGGGCGGCGGGATGAAGCGCTTGAGGAAGCAGACTGGGAAATGTCGGCAGGCGGCGGCTCTCGGGAGCGCGACTGTGCAGGTTTCGACCCTGAGACGCGGGATCCCGAGTTCGAGTGGCTGTCAAGACACACATGCGCGGAGCCCGATGCCGAGTCGCCGAAACCGCGCGAGTGGAATTCGGACCCGGGTACCCGGAGTGGGTTCGCGCCCATTGGAGGAAGCAGGCATCAGTCTCGGAATGCACGGCGAGGATTGGAGGATGTCTTGCCTAGGGGCCCTGGCTACATTCTGCTTTGGGGCGGAGCCGCTGAGGAGCCGGAATTTCTATTGGCGGAGGAGATGCATGAGGGCGGGCCTGTGAGAGGACGGAGACCGCTCGCCGGGCGGCGGGATGAAGCGCTTGAGGAAGCAGACTGGGAAATGTCGGCAGGCGGCGGCTCTCGGGAGCGCGACTGTGCA
Peptide sequence:
MHEGGPVRGRRPLAGRRDEALEEADWEMSAGGGSRERDCAGFDPETRDPEFEWLSRHTCAEPDAESPKPREWNSDPGTRSGFAPIGGSRHQSRNARRGLEDVLPRGPGYILLWGGAAEEPEFLLAEEMHEGGPVRGRRPLAGRRDEALEEADWEMSAGGGSRERDCA
IRESfinder result
IRESfinder score
0.068201994
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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