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mmu_circInpp4a_366
Basic information of circular RNA
PanCircBase ID:
mmu_circInpp4a_366
mm39_Chr. Position ID:
chr1|37432023|37438001|+
Gene name:
Inpp4a
Transcript:
ENSMUST00000027287.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
23,24,25
Exon/intron offsets:
0,3127,5823
Genomic length:
5978
Exon/intron sizes in nt:
9,81,13,155
Flanking exon/introns:
chr1:37429270-37432023|chr1:37438001-37447207
Splice length:
366
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGGAGATACGTCCTTACAAGAAGTTATCAATGTGGAGAGCTTGGTGCGGTTAAATTCCTACTTTGAGCAGTTTAAGGAGGTTTTGCCGGAGGACTGTCTACCTCGATCTCGGAGTCAGACCTGCCTTCCAGAGCTGCTGCGGTTCCTGGGACAGAATGTCCATGCACGCAAGAATAAGAATGTGGACATCCTCTGGCAAGCTGCTGAGGTCTGTCGCCGCCTTAACGGGGTCCGATTCACCAGCTGCAAGAGTGCCAAGGACCGCACAGCCATGTCGGTGACCCTGGAGCAGTGTCTGATCCTGCAGCACGAGCACGGCATGGCCCCGCAGGTCTTCACACAAGCCCTGGAGTGCATGCGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGTCTGATCCTGCAGCAC
60.04
CCTCCGGCAAAACCTCCTTA
59.67
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGTGTCTGATCCTGCAGCA
60.04
CCTCCGGCAAAACCTCCTTA
59.67
169
PCR template sequence:
GCACAGCCATGTCGGTGACCCTGGAGCAGTGTCTGATCCTGCAGCACGAGCACGGCATGGCCCCGCAGGTCTTCACACAAGCCCTGGAGTGCATGCGCAGGTTTGGAGATACGTCCTTACAAGAAGTTATCAATGTGGAGAGCTTGGTGCGGTTAAATTCCTACTTTGAGCAGTTTAAGGAGGTTTTGCCGGAGGACTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-383-5p(1), mmu-miR-98-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTGCATGCGCAGGTTTGGAGATACG
siRNA sets
TCTCCAAACCTGCGCATGC, GTATCTCCAAACCTGCGCA, CTCCAAACCTGCGCATGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCINPP4A_366_ORF_1
1.2689
0.470914699
0.910151985
456
ORF sequence:
ATGTCGGTGACCCTGGAGCAGTGTCTGATCCTGCAGCACGAGCACGGCATGGCCCCGCAGGTCTTCACACAAGCCCTGGAGTGCATGCGCAGGTTTGGAGATACGTCCTTACAAGAAGTTATCAATGTGGAGAGCTTGGTGCGGTTAAATTCCTACTTTGAGCAGTTTAAGGAGGTTTTGCCGGAGGACTGTCTACCTCGATCTCGGAGTCAGACCTGCCTTCCAGAGCTGCTGCGGTTCCTGGGACAGAATGTCCATGCACGCAAGAATAAGAATGTGGACATCCTCTGGCAAGCTGCTGAGGTCTGTCGCCGCCTTAACGGGGTCCGATTCACCAGCTGCAAGAGTGCCAAGGACCGCACAGCCATGTCGGTGACCCTGGAGCAGTGTCTGATCCTGCAGCACGAGCACGGCATGGCCCCGCAGGTCTTCACACAAGCCCTGGAGTGCATGCGC
Peptide sequence:
MSVTLEQCLILQHEHGMAPQVFTQALECMRRFGDTSLQEVINVESLVRLNSYFEQFKEVLPEDCLPRSRSQTCLPELLRFLGQNVHARKNKNVDILWQAAEVCRRLNGVRFTSCKSAKDRTAMSVTLEQCLILQHEHGMAPQVFTQALECMR
IRESfinder result
IRESfinder score
0.319399724
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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